Cited by 80Algal biology and biofuel productionLipid metabolism and biosynthesisAquaculture Nutrition and Growth

Characterization of lipid and fatty acids composition of Chlorella zofingiensis in response to nitrogen starvation

Zhu, Shunni, Wang, Yajie, Shang, Changhua, Wang, Zhongming, Xu, Jingliang, Yuan, Zhenhong·2015·Journal of Bioscience and Bioengineering

Abstract

Cellular biochemical composition of the microalga Chlorella zofingiensis was studied under favorable and nitrogen starvation conditions, with special emphasis on lipid classes and fatty acids distribution. When algal cells were grown in nitrogen-free medium (N stress), the increase in the contents of lipid and carbohydrate while a decrease in protein content was detected. Glycolipids were the major lipid fraction (50.7% of total lipids) under control condition, while neutral lipids increased to be predominant (86.7% of total lipids) under N stress condition. Triacylglycerol (TAG) content in N stressed cells was 27.3% dw, which was over three times higher than that obtained under control condition. Within neutral lipids fraction, monounsaturated fatty acids (MUFA) were the main group (40.6%) upon N stress, in which oleic acid was the most representative fatty acids (34.5%). Contrarily, glycolipids and phospholipids showed a higher percentage of polyunsaturated fatty acids (PUFA). Lipid quality assessment indicated the potential of this alga as a biodiesel feedstock when its neutral lipids were a principal lipid fraction. The results demonstrate that the neutral lipids content is key to determine the suitability of the microalga for biodiesel, and the stress cultivation is essential for lipid quality.

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Crossref · 10.1016/j.jbiosc.2014.12.018

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Authors
Zhu, S., Wang, Y., Shang, C., Wang, Z., Xu, J., & Yuan, Z.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
Yuan, Zhenhong
Venue
Journal of Bioscience and Bioengineering
Publisher
Elsevier BV
Published
2015-08
Volume
120
Issue
2
Pages
205-209
ISSN
  • 1347-4421 (ISSN-L)
  • 1389-1723
OpenAlex
W2021707313
Cited by
80
References
35
Citations by year
2026: 4 · 2025: 3 · 2024: 10 · 2023: 6 · 2022: 8 · 2021: 12 · 2020: 6 · 2019: 13 · 2018: 8 · 2017: 5 · 2016: 3 · 2015: 2
FWCI
1.78
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Bioscience and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repository of Guangzhou Institute of Energy Research, Chinese Academy of Sciences (submittedVersion)
Funders
  • National Natural Science Foundation of China (31100189)
  • National Basic Research Program of China (2011CB200905)
  • National Key Technology R&D Program of China (2011BAD14B03)
  • National High-tech R&D Program (2013AA065803)
  • Scientific Research Foundation
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
Chlorella zofingiensis, nitrogen starvation, fatty acid composition, triglycerides, neutral lipids, glycolipids, phospholipids, oleic acid, monounsaturated fatty acids, polyunsaturated fatty acids, lipid quality, biodiesel feedstock
MeSH terms
Carbohydrates, Chlorella, Fatty Acids, Glycolipids, Lipids, Nitrogen, Phospholipids, Proteins, Triglycerides, Oleic Acid, Biofuels

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