Investigating the effects of eleven key physicochemical factors on growth and lipid accumulation of Chlorella sp. as a feedstock for biodiesel production
Abstract
Biodiesel, as a renewable and eco-friendly energy source that can be produced through algae oil esterification, has recently received much attention. Maximization of algal biomass and lipid content is crucial for commercial biodiesel production. In this study, Chlorella sp. PG96, a microalgal strain isolated from urban wastewater, was identified considering its morphological and molecular characteristics. Fractional factorial design (2 11-7) was employed to screen medium and environmental factors for achieving high lipid productivity. The effects of eleven factors including light intensity, light spectrum, aeration rate, temperature, salinity, NaHCO 3, CO 2, NaNO 3, NH 4 Cl, MgSO 4.7H 2 O, and K 2 HPO 4 and their interactions on growth characteristics of Chlorella sp. PG96 (biomass and lipid production) were statistically assessed. Based on the experimental results, lipid productivity was at its maximum (54.19 ± 8.40 mg lipid L-1 day-1) under a combination of high levels of all factors. The analysis also showed that physical parameters of light intensity and temperature were more effective on algal growth compared to nutritional parameters. Furthermore, nitrogen source of ammonium and carbon source of bicarbonate played more significant roles in biomass and lipid production, compared with nitrate and CO 2, respectively. Although the effect of sulfur limitation on cellular growth was similar to phosphorus deficiency, S-limitation had a greater impact on lipid accumulation. The interaction between NaHCO 3 and NH 4 Cl was the most prominent interaction affecting all responses. It is concluded that Chlorella sp. PG96 at a high level of light intensity and temperature (22500 Lux and 32 °C, respectively) can be a prospective candidate for biodiesel production.
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NaHCO3 increases lipid content while concomitantly enhancing the rate of CO2 fixation in Chlorella sp.
“Furthermore, Roya et al. has also suggested that NaHCO3 increases lipid content while concomitantly enhancing the rate of CO2 fixation in Chlorella sp. [100].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [100]
Some microalgae even prefer NaHCO3 to CO2 for lipid biosynthesis.
“Besides, some microalgae even prefer NaHCO3 to CO2 for lipid biosynthesis [100].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [100]
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“Furthermore, Roya et al . has also suggested that NaHCO 3 increases lipid content while concomitantly enhancing the rate of CO 2 fixation in Chlorella sp. [ 100].”
Background
- Authors
- Parichehreh, R., Gheshlaghi, R., Mahdavi, M. A., & Kamyab, H.
- Affiliations
- Ferdowsi University of Mashhad (IR)
- University of Technology Malaysia (MY)
- Corresponding author
- Gheshlaghi, Reza
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 2021-11
- Volume
- 340
- Pages
- 64-74
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W3196880417
- Cited by
- 47
- References
- 81
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 11 · 2023: 7 · 2022: 14 · 2021: 3
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- microalgae, biodiesel feedstock, lipid accumulation, algal growth, culture medium optimization, light intensity, light spectrum, temperature, salinity, nitrogen source, ammonium, bicarbonate, sulfur limitation, phosphorus deficiency, fractional factorial design
- MeSH terms
- Chlorella, Lipids, Prospective Studies, Biomass, Biofuels, Microalgae
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