Culture conditions affect eicosapentaenoic acid content of Chlorella minutissima
Abstract
Abstract Marine chlorella known to contain high amount of eicosapentaenoic acid (20:5ω3) are a potential natural source of this fatty acid for health foods and pharmaceuticals. The effect of culture conditions on lipid content and fatty acid composition ofChlorella minutissima, therefore, was investigated. Lipid content of cells grown at 25 C was 60% higher than that of the cells grown at 20 C. Fatty acid composition was affected by culture temperature and supplementation of NaCl. Eicosapentaenoic acid 20:5ω3 content was 45% (w/w) in the cells grown at 20 C, whereas that of the cells grown at 25 C was only 20% (w/w). Supplementation with NaCl also increased the percentage of 20:5ω3 acid to the same extent.
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EPA is produced in high amounts in Chlorella minutissima, and its production can be increased by reducing temperature and increasing salinity.
“EPA is produced in high amounts in the marine green algae Chlorella minutissima, and the production of this fatty acid can be increased by reducing temperature and increasing salinity (Seto et al., 1984)”
From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Seto et al., 1984
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Bimbo, A. P. (2013) · Elsevier eBooks · doi:10.1533/9780857098863.1.27 · Cited by 33 · see citing works
Shim, S. J., Hong, M. E., Chang, W. S., & Sim, S. J. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.122944 · Cited by 33 · see citing works
Zittelli, G. C., Lavista, F., Bastianini, A., Rodolfi, L., VINCENZINI, M. T., & Tredici, M. R. (1999) · Progress in industrial microbiology · doi:10.1016/s0079-6352(99)80122-2 · Cited by 33 · see citing works
- Authors
- Seto, A., Wang, H. L., & Hesseltine, C. W.
- Affiliations
- Agricultural Research Service (US)
- Venue
- Journal of the American Oil Chemists Society
- Publisher
- Wiley
- Published
- 1984-05
- Volume
- 61
- Issue
- 5
- Pages
- 892-894
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W1972543099
- Cited by
- 177
- References
- 17
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 8 · 2019: 4 · 2018: 3 · 2017: 6 · 2016: 3 · 2015: 6 · 2014: 4 · 2013: 7 · 2012: 6
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Oil Chemists Society (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Chlorella minutissima, eicosapentaenoic acid, omega-3 fatty acids, culture temperature, salinity, sodium chloride supplementation, lipid content, fatty acid composition, microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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