The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer
Open accessAbstract
BACKGROUND & AIMS: The successful culture of intestinal organoids has greatly enhanced our understanding of intestinal stem cell physiology and enabled the generation of novel intestinal disease models. Although of tremendous value, intestinal organoid culture systems have not yet fully recapitulated the anatomy or physiology of the in vivo intestinal epithelium. The aim of this work was to re-create an intestinal epithelium with a high density of polarized crypts that respond in a physiologic manner to addition of growth factors, metabolites, or cytokines to the basal or luminal tissue surface as occurs in vivo. METHODS: A self-renewing monolayer of human intestinal epithelium was cultured on a collagen scaffold microfabricated with an array of crypt-like invaginations. Placement of chemical factors in either the fluid reservoir below or above the cell-covered scaffolding created a gradient of that chemical across the growing epithelial tissue possessing the in vitro crypt structures. Crypt polarization (size of the stem/proliferative and differentiated cell zones) was assessed in response to gradients of growth factors, cytokines, and bacterial metabolites. RESULTS: Chemical gradients applied to the shaped human epithelium re-created the stem/proliferative and differentiated cell zones of the in vivo intestine. Short-chain fatty acids applied as a gradient from the luminal side confirmed long-standing hypotheses that butyrate diminished stem/progenitor cell proliferation and promoted differentiation into absorptive colonocytes. A gradient of interferon-γ and tumor necrosis factor-α significantly suppressed the stem/progenitor cell proliferation, altering crypt formation. CONCLUSIONS: The in vitro human colon crypt array accurately mimicked the architecture, luminal accessibility, tissue polarity, cell migration, and cellular responses of in vivo intestinal crypts.
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Cites
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
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“and is thought to act as a Notch activator.(43,44) In contrast, DAPT, a Notch inhibitor, increased Muc2 expression, suggesting that Notch inhibition increased Goblet cell production under these conditions.”
BackgroundResultThe hope and the hype of organoid research
Huch, M., Knoblich, J. A., Lutolf, M. P., & Martinez-Arias, A. (2017) · Development · doi:10.1242/dev.150201 · Cited by 409 · cited 1×
“These characteristics preclude the use of the organoid system in numerous applications and a true living construct suitable for assay of dietary metabolites, cytokines, microorganisms, and drug interactions has not been available.(23,24)”
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Cited by
Progress and potential in organoid research
Rossi, G., Manfrin, A., & Lutolf, M. P. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0051-9 · Cited by 1,182
Park, S. E., Georgescu, A., & Huh, D. (2019) · Science · doi:10.1126/science.aaw7894 · Cited by 847
Homeostatic mini-intestines through scaffold-guided organoid morphogenesis
Nikolaev, M., Mitrofanova, O., Broguiere, N., Geraldo, S., Dutta, D., Tabata, Y., Elci, B., Brandenberg, N., Kolotuev, I., Gjorevski, N., et al. (2020) · Nature · doi:10.1038/s41586-020-2724-8 · Cited by 755
Tissue geometry drives deterministic organoid patterning
Gjorevski, N., Nikolaev, M., Brown, T. E., Mitrofanova, O., Brandenberg, N., DelRio, F. W., Yavitt, F. M., Liberali, P., Anseth, K. S., & Lutolf, M. P. (2022) · Science · doi:10.1126/science.aaw9021 · Cited by 505
Engineering Stem Cell Self-organization to Build Better Organoids
Brassard, J. A., & Lutolf, M. P. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.05.005 · Cited by 373
Metadata
- Authors
- Wang, Y., Kim, R., Gunasekara, D. B., Reed, M. I., DiSalvo, M., Nguyen, D. L., Bultman, S. J., Sims, C. E., Magness, S. T., & Allbritton, N. L.
- Venue
- Cellular and Molecular Gastroenterology and Hepatology
- Publisher
- Elsevier BV
- Published
- 2018
- Volume
- 5
- Issue
- 2
- Pages
- 113-130
- ISSN
- 2352-345X (ISSN-L)
- OpenAlex
- W2766670989
- Cited by
- 185
- References
- 14
- FWCI
- 15.92
- Type
- journal article
- Language
- en
- Open access
- gold
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- Wang, Y., Kim, R., Gunasekara, D. B., Reed, M. I., DiSalvo, M., Nguyen, D. L., Bultman, S. J., Sims, C. E., Magness, S. T., & Allbritton, N. L. (2018). Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer. Cellular and Molecular Gastroenterology and Hepatology. https://doi.org/10.1016/j.jcmgh.2017.10.007
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
