1,2 propanediol utilization by Lactobacillus reuteri DSM 20016, role in bioconversion of glycerol to 1,3 propanediol, 3-hydroxypropionaldehyde and 3-hydroxypropionic acid
Open accessAbstract
The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose. The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72 h and the metabolites produced during the process were detected using HPLC. 1,2 Propanediol was completely converted to propionaldhyde in a time dependent fashion, this process had a higher rate in presence of glucose. Consequently the produced propionaldhyde was converted to propionic acid and propanol in a skewed equimolar manner. In presence of glucose: acetic acid, lactic acid, succinic acid and ethanol were detected while in absence of glucose only minute amounts of acetic acid and lactic acid were detected which indicates presence of different metabolic pathways for glucose and 1,2 propanediol metabolism. Resting cells of L. reuteri induced in presence of 1,2 propanediol have shown significant capabilities to convert aqueous glycerol to 1,3 propanediol, 3-hydroxypropionaldhyde and a compound proposed to be 3-hydroxypropionic acid as detected by gas chromatographic technique.
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Propionic acid synthesis follows a specific pathway putatively involving Lactobacillus diolivorans or Lactobacillus reuteri.
“The synthesis of propionic acid follows a specific pathway, putatively involving other members of the lactic acid bacterial population, i.e., Lactobacillus diolivorans [68] or Lactobacillus reuteri [69].”
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- Authors
- Amin, H. M., Hashem, A. M., Ashour, M. S., & Hatti-Kaul, R.
- Affiliations
- October University of Modern Sciences and Arts (EG)
- Lund University (SE)
- Corresponding author
- Amin, Heba M.
- Venue
- Journal of Genetic Engineering and Biotechnology
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 11
- Issue
- 1
- Pages
- 53-59
- ISSN
- 1687-157X (ISSN-L)
- OpenAlex
- W1967987898
- Cited by
- 40
- References
- 41
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 2
- FWCI
- 0.75
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Genetic Engineering and Biotechnology (publishedVersion, cc-by-nc-nd)
- Lund University Publications (Lund University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Catalysis for Biomass Conversion
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- Lactobacillus reuteri, glycerol bioconversion, 1,3-propanediol, 3-hydroxypropionaldehyde, 3-hydroxypropionic acid, propionaldehyde, propionic acid, glucose metabolism, metabolic pathways
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