Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera
Abstract
Western corn rootworm (WCR), Diabrotica virgifera virgifera, is one of the most significant pests of corn in the United States. Although transgenic solutions exist, increasing resistance concerns make the discovery of novel solutions essential. In order to find a novel protein with high activity and a new mode of action, a large microbial collection was surveyed for toxicity to WCR using in vitro bioassays. Cultures of strain ATX2024, identified as Chromobacterium piscinae, had very high activity against WCR larvae. The biological activity from the strain was purified using chromatographic techniques and fractions were tested against WCR larvae. Proteins in the final active fraction were identified by mass spectrometry and N-terminal sequencing and matched to the genome of ATX2024. A novel 58.9kDa protein, identified by this approach, was expressed in a recombinant expression system and found to have specific activity against WCR. Transgenic corn events containing this gene showed good protection against root damage by WCR, with average scores ranging between 0.01 and 0.04 on the Iowa State node injury scale. Sequence analysis did not reveal homology to any known insecticidal toxin, suggesting that this protein may act in a novel way to control WCR. The new WCR active protein is named GNIP1Aa, for Gram Negative Insecticidal Protein.
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Effective toxins have been isolated from other bacteria including species of Pseudomonas and Chromobacter that might be expressed in future insect-resistant GE plants.
“In addition to Bt, effective toxins have also been isolated from other bacteria including species of Pseudomonas (Anderson et al., 2018) and Chromobacter (Sampson et al. 2017) that might be expressed in future insect-resistant GE plants.”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Sampson et al. 2017
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- Authors
- Sampson, K., Zaitseva, J., Stauffer, M., Vande Berg, B., Guo, R., Tomso, D., McNulty, B., Desai, N., & Balasubramanian, D.
- Affiliations
- Bayer (United States) (US)
- Corresponding author
- Sampson, Kimberly
- ORCID
- Zaitseva, Jelena
- Venue
- Journal of Invertebrate Pathology
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 142
- Pages
- 34-43
- ISSN
- 0022-2011 (ISSN-L)
- OpenAlex
- W2542368933
- Cited by
- 30
- References
- 87
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 2
- FWCI
- 1.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Invertebrate Pathology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Entomopathogenic Microorganisms in Pest Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- western corn rootworm, insecticidal proteins, microbial insecticides, transgenic maize, insecticidal protein expression
- MeSH terms
- Animals, Coleoptera, Chromobacterium, Zea mays, Endotoxins, Insecticides, Pest Control, Biological, Mass Spectrometry, Polymerase Chain Reaction, Plants, Genetically Modified
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