Cited by 30Insect Resistance and GeneticsEntomopathogenic Microorganisms in Pest ControlInsect Pest Control Strategies

Discovery of a novel insecticidal protein from Chromobacterium piscinae, with activity against Western Corn Rootworm, Diabrotica virgifera virgifera

Sampson, Kimberly, Zaitseva, Jelena, Stauffer, Maria, Vande Berg, Brian, Guo, Rong, Tomso, Daniel, McNulty, Brian, Desai, Nalini +1 more·2017·Journal of Invertebrate Pathology

Abstract

Western corn rootworm (WCR), Diabrotica virgifera virgifera, is one of the most significant pests of corn in the United States. Although transgenic solutions exist, increasing resistance concerns make the discovery of novel solutions essential. In order to find a novel protein with high activity and a new mode of action, a large microbial collection was surveyed for toxicity to WCR using in vitro bioassays. Cultures of strain ATX2024, identified as Chromobacterium piscinae, had very high activity against WCR larvae. The biological activity from the strain was purified using chromatographic techniques and fractions were tested against WCR larvae. Proteins in the final active fraction were identified by mass spectrometry and N-terminal sequencing and matched to the genome of ATX2024. A novel 58.9kDa protein, identified by this approach, was expressed in a recombinant expression system and found to have specific activity against WCR. Transgenic corn events containing this gene showed good protection against root damage by WCR, with average scores ranging between 0.01 and 0.04 on the Iowa State node injury scale. Sequence analysis did not reveal homology to any known insecticidal toxin, suggesting that this protein may act in a novel way to control WCR. The new WCR active protein is named GNIP1Aa, for Gram Negative Insecticidal Protein.

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Crossref · 10.1016/j.jip.2016.10.004

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  • Effective toxins have been isolated from other bacteria including species of Pseudomonas and Chromobacter that might be expressed in future insect-resistant GE plants.

    “In addition to Bt, effective toxins have also been isolated from other bacteria including species of Pseudomonas (Anderson et al., 2018) and Chromobacter (Sampson et al. 2017) that might be expressed in future insect-resistant GE plants.”

    From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Sampson et al. 2017

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Authors
Sampson, K., Zaitseva, J., Stauffer, M., Vande Berg, B., Guo, R., Tomso, D., McNulty, B., Desai, N., & Balasubramanian, D.
Affiliations
Bayer (United States) (US)
Corresponding author
Sampson, Kimberly
Venue
Journal of Invertebrate Pathology
Publisher
Elsevier BV
Published
2017-01
Volume
142
Pages
34-43
ISSN
0022-2011 (ISSN-L)
OpenAlex
W2542368933
Cited by
30
References
87
Citations by year
2025: 4 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 2
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Invertebrate Pathology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Entomopathogenic Microorganisms in Pest Control
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords
western corn rootworm, insecticidal proteins, microbial insecticides, transgenic maize, insecticidal protein expression
MeSH terms
Animals, Coleoptera, Chromobacterium, Zea mays, Endotoxins, Insecticides, Pest Control, Biological, Mass Spectrometry, Polymerase Chain Reaction, Plants, Genetically Modified

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