Cited by 117Neuroendocrine regulation and behaviorHuman-Animal Interaction StudiesAnimal Behavior and Welfare Studies

The DOI matches its Crossref record; 55 of the 56 references it cites were found in a registry or an index.

Validation of salivary oxytocin and vasopressin as biomarkers in domestic dogs

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Abstract

BACKGROUND: Oxytocin (OT) and Vasopressin (AVP) are phylogenetically conserved neuropeptides with effects on social behavior, cognition and stress responses. Although OT and AVP are most commonly measured in blood, urine and cerebrospinal fluid (CSF), these approaches present an array of challenges including concerns related to the invasiveness of sample collection, the potential for matrix interference in immunoassays, and whether samples can be collected at precise time points to assess event-linked endocrine responses. NEW METHOD: We validated enzyme-linked immunosorbent assays (ELISAs) for the measurement of salivary OT and AVP in domestic dogs. RESULTS: Both OT and AVP were present in dog saliva and detectable by ELISA and high performance liquid chromatography - mass spectrometry (HPLC-MS). OT concentrations in dog saliva were much higher than those typically detected in humans. OT concentrations in the same samples analyzed with and without sample extraction were highly correlated, but this was not true for AVP. ELISA validation studies revealed good accuracy and parallelism, both with and without solid phase extraction. Collection of salivary samples with different synthetic swabs, or following salivary stimulation or the consumption of food led to variance in results. However, samples collected from the same dogs using different techniques tended to be positively correlated. We detected concurrent elevations in salivary and plasma OT during nursing. COMPARISON WITH EXISTING METHODS: There are currently no other validated methods for measuring OT/AVP in dog saliva. CONCLUSIONS: OT and AVP are present in dog saliva, and ELISAs for their detection are methodologically valid.

Overview

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Crossref · 10.1016/j.jneumeth.2017.08.033

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55 of 55 references verified0 citation errors · 0 not checkable

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Metadata

Authors
MacLean, E. L., Gesquiere, L. R., Gee, N., Levy, K., Martin, W. L., & Carter, C. S.
Affiliations
  • University of Arizona (US)
  • Duke University (US)
  • State University of New York (US)
  • Canine Companions for Independence (US)
  • Indiana University Bloomington (US)
Corresponding author
MacLean, Evan L.
Venue
Journal of Neuroscience Methods
Publisher
Elsevier BV
Published
2018-01
Volume
293
Pages
67-76
ISSN
0165-0270 (ISSN-L)
OpenAlex
W2951357445
Cited by
117
References
56
Citations by year
2026: 7 · 2025: 13 · 2024: 17 · 2023: 6 · 2022: 13 · 2021: 19 · 2020: 14 · 2019: 14 · 2018: 11
FWCI
16.48
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience Methods (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • UA Campus Repository (The University of Arizona) (acceptedVersion)
Funders
Waltham Centre for Pet Nutrition
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Human-Animal Interaction Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Behavior and Welfare Studies
Keywords
salivary oxytocin, dogs, biomarkers, ELISA validation, neuropeptides, stress response, saliva collection methods, solid-phase extraction, plasma oxytocin
MeSH terms
Animals, Chromatography, High Pressure Liquid, Dogs, Enzyme-Linked Immunosorbent Assay, Female, Lactation, Male, Oxytocin, Saliva, Mass Spectrometry, Vasopressins, Biomarkers

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