Cold stress induced morphological microglial activation and increased IL-1β expression in astroglial cells in rat brain
Abstract
The present study investigated the possible impact of cold stress on the immune functions of the brain. Wistar rats were exposed to 4°C for 2h prior to analysis of immunohistochemical analysis of OX-42 and IL-1β, which are markers of microglia and inflammation, respectively. Exposure to cold stress induced morphological microglial activation in as early as 30 min, and the activation lasted up to 2h following the stress. In addition, increased IL-1β-immunoreactivity was detected in the hippocampus and hypothalamus. However, IL-1β was not co-localized with microglia, and was predominantly expressed in astroglia. The present study provides the first evidence that cold stress contributes to neuro-immunomodulation in the brain through microglial activation and expression of IL-1β in astroglia.
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Following incubation at 4 °C for longer than 30 min, microglia soma enlarged, but recovered normal morphology within 2 h.
“it has been reported that following incubation at 4 °C for longer periods of time (> 30 min) microglia soma enlarged, but recovered normal morphology within 2 h64.”
From Microglia remodel synapses by presynaptic trogocytosis and spine head filopodia induction (2018) · cites this paper as [64]
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- Authors
- Sugama, S., Takenouchi, T., Fujita, M., Kitani, H., & Hashimoto, M.
- Affiliations
- Nippon Medical School (JP)
- Institute of Agrobiological Sciences (JP)
- Corresponding author
- Sugama, Shuei
- Venue
- Journal of Neuroimmunology
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 233
- Issue
- 1-2
- Pages
- 29-36
- ISSN
- 0165-5728 (ISSN-L)
- OpenAlex
- W2121667809
- Cited by
- 55
- References
- 39
- Citations by year
- 2026: 3 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 1 · 2019: 3 · 2018: 7 · 2017: 8 · 2016: 5 · 2015: 2 · 2014: 5 · 2013: 6 · 2012: 4
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroimmunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Thermal Regulation in Medicine
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Keywords
- cold stress, microglial activation, IL-1β, astroglial cells, neuroinflammation, hippocampus, hypothalamus, OX-42, cold exposure
- MeSH terms
- Animals, Brain, Cold Temperature, Disease Models, Animal, Gliosis, Male, Stress, Physiological, Neuroimmunomodulation, Up-Regulation, Rats, Wistar, Microglia, Rats, Interleukin-1beta, Cold-Shock Response
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