Cyasterone accelerates fracture healing by promoting MSCs migration and osteogenesis
Open accessAbstract
BACKGROUND: Mesenchymal Stem Cells (MSCs) therapy has become a new coming focus of clinical research in regenerative medicine. However, only a small number of implanted MSCs could successfully reach the injured areas. The previous studies have shown that fracture healing time is inversely proportional to concentration of MSCs in injured tissue. METHODS: The migration and osteogenesis of MSCs were assessed by transwell assay and Alizarin Red S staining. Levels of gene and protein expression were checked by qPCR and Western Blot. On the other hand, the enhanced migration ability of MSCs induced by Cyasterone was retarded by CXCR4 siRNA. In addition, the rat model of femoral fracture was established to evaluate the effect of Cyasterone on fracture healing. What's more, we also checked the effect of Cyasterone on mobilisation of MSCs in vivo. RESULTS: The results showed that Cyasteron increased the number of MSCs in peripheral blood. The concentrations of SDF-1α in serum at different time points were determined by ELISA assay. Micro-CT and histological analysis were used to evaluate the fractured femurs.Our results showed that Cyasterone could promote the migration and osteogenesis capacities of MSCs. The fractured femurs healed faster with treatment of Cyasterone. Meanwhile, Cyasterone could significantly increase the level of SDF-1α in rats with femur fracture. CONCLUSION: Cyasterone could promote migration and osteogenesis of MSCs, and most importantly, it could accelerate bone fracture healing.Translational Potential statement: These findings provide evidence that Cyasterone could be used as a therapeutic reagent for MSCs mobilisation and osteogenesis. What's more, it could acclerate fracture healing.
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There is a lack of evidence from clinical studies in patients for selective prostaglandin agonists, cyasterone, statins, and anti-sclerostin antibodies as fracture-healing accelerators.
“Other drugs that have been considered to accelerate fracture healing include selective prostaglandin agonists (111), cyasterone (112), statins, and anti-sclerostin antibodies (113), but there is a lack of evidence from clinical studies in patients.”
From Methods to accelerate fracture healing – a narrative review from a clinical perspective (2024) · cites this paper as [112]
The Discussion identifies cyasterone as a proposed way to promote fracture healing, and the Abstract Methods describe cell experiments and a rat fracture model, not a study in patients. The paper therefore supports cyasterone’s inclusion among potential accelerators, but it does not establish the broader claim that clinical evidence for cyasterone is lacking. (LLM, conf=0.84)
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“Other drugs that have been considered to accelerate fracture healing include selective prostaglandin agonists (111), cyasterone (112), statins, and anti-sclerostin antibodies (113), but there is a lack of evidence from clinical studies in patients.”
Background
- Authors
- Zhu, J., Liu, Y., Chen, C., Chen, H., Huang, J., Luo, Y., Zhao, K., Chen, D., Xu, Z., Li, W., Zhang, X., Xiong, Y., Xu, L., & Wang, B.
- Affiliations
- Guangzhou University of Chinese Medicine (CN)
- University of Hong Kong (HK)
- Jiangmen Wuyi Traditional Chinese Medicine Hospital (CN)
- Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine (CN)
- Corresponding author
- Xu, Liangliang
- Wang, Bin
- ORCID
- Venue
- Journal of Orthopaedic Translation
- Publisher
- Elsevier BV
- Published
- 2021-05
- Volume
- 28
- Pages
- 28-38
- ISSN
- 2214-031X (ISSN-L)
- PMC
- PMC7905397
- OpenAlex
- W3131272701
- Cited by
- 33
- References
- 35
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 7 · 2023: 4 · 2022: 9 · 2021: 4
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Orthopaedic Translation (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (81873326)
- Natural Science Foundation of Guangdong Province (2017A030313729)
- Science and Technology Planning Project of Guangdong Province (2017A050506046)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Epidemiology › Bone fractures and treatments
- Keywords
- cyasterone, fracture healing, mesenchymal stem cells, cell migration, bone formation, SDF-1α, CXCR4, stem cell mobilization, femoral fracture, bone regeneration
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