Cited by 88Fullerene Chemistry and ApplicationsSkin Protection and AgingEffects of Radiation Exposure

Super-Highly Hydroxylated Fullerene Derivative Protects Human Keratinocytes from UV-Induced Cell Injuries Together with the Decreases in Intracellular ROS Generation and DNA Damages

Saitoh, Yasukazu, Miyanishi, Akifumi, Mizuno, Hiromi, Kato, Shinya, Aoshima, Hisae, Kokubo, Ken, Miwa, Nobuhiko·2011·Journal of Photochemistry and Photobiology B Biology

Abstract

Polyhydroxylated fullerenes (fullerenols: C(60)(OH)(n)) are known as the major water-soluble fullerene derivatives which possess particular significance as free radical scavengers or antioxidants in biological systems. Recently, the novel polyhydroxylated fullerene (C(60) (OH)(44)·8H(2)O: SHH-F) was successfully synthesized. In the present study, we investigated the radical-scavenging effects and cytoprotective effects of three types of fullerenols (C(60)(OH)(6-12): LH-F, C(60) (OH)(32-34)·7H(2)O: HH-F, and C(60) (OH)(44)·8H(2)O: SHH-F) on UV-irradiation-induced cell injuries. HH-F and SHH-F exerted hydroxyl-radical scavenging activities as shown by DMPO-spin trap/ESR method, more markedly than LH-F. UVA or UVB irradiation-induced injuries in human skin keratinocytes HaCaT were significantly suppressed by HH-F and SHH-F, but scarcely by LF-H. The cytoprotective effects of SHH-F had a tendency to be superior to that of HH-F. And the cytoprotective effects of SHH-F against UVB-induced injuries were more effective than those of UVA. Irradiation with UVB to HaCaT cells was shown to cause rapid increases in cell-injury-associated symptoms such as intracellular oxidative stress levels, the formation of cyclobutane pyrimidine dimers and chromatin condensation, all of which were repressed by SHH-F. Thus, UVB-induced diverse harmful effects could be prevented by SHH-F, which was suggested to exert the cytoprotective effects through intracellular reactive oxygen species-scavenging in the keratinocytes.

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Crossref · 10.1016/j.jphotobiol.2010.09.006

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Authors
Saitoh, Y., Miyanishi, A., Mizuno, H., Kato, S., Aoshima, H., Kokubo, K., & Miwa, N.
Affiliations
  • Prefectural University of Hiroshima (JP)
  • The University of Osaka (JP)
Corresponding author
Miwa, Nobuhiko
Venue
Journal of Photochemistry and Photobiology B Biology
Publisher
Elsevier BV
Published
2011-01
Volume
102
Issue
1
Pages
69-76
ISSN
1011-1344 (ISSN-L)
OpenAlex
W1981863487
Cited by
86
References
68
Citations by year
2026: 3 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 14 · 2012: 7
FWCI
3.96
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Photochemistry and Photobiology B Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Organic Chemistry › Fullerene Chemistry and Applications
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
Keywords
polyhydroxylated fullerenes, fullerenol, keratinocytes, HaCaT keratinocytes, UV-B radiation, UVA, hydroxyl radical scavenging, reactive oxygen species, oxidative stress, DNA damage, cyclobutane pyrimidine dimers, cytoprotection, antioxidants
MeSH terms
Chromatin, DNA Damage, Humans, Hydroxylation, Pyrimidine Dimers, Ultraviolet Rays, Keratinocytes, Free Radical Scavengers, Reactive Oxygen Species, Hydroxyl Radical, Cytoprotection, Fullerenes, Intracellular Space

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