Super-Highly Hydroxylated Fullerene Derivative Protects Human Keratinocytes from UV-Induced Cell Injuries Together with the Decreases in Intracellular ROS Generation and DNA Damages
Abstract
Polyhydroxylated fullerenes (fullerenols: C(60)(OH)(n)) are known as the major water-soluble fullerene derivatives which possess particular significance as free radical scavengers or antioxidants in biological systems. Recently, the novel polyhydroxylated fullerene (C(60) (OH)(44)·8H(2)O: SHH-F) was successfully synthesized. In the present study, we investigated the radical-scavenging effects and cytoprotective effects of three types of fullerenols (C(60)(OH)(6-12): LH-F, C(60) (OH)(32-34)·7H(2)O: HH-F, and C(60) (OH)(44)·8H(2)O: SHH-F) on UV-irradiation-induced cell injuries. HH-F and SHH-F exerted hydroxyl-radical scavenging activities as shown by DMPO-spin trap/ESR method, more markedly than LH-F. UVA or UVB irradiation-induced injuries in human skin keratinocytes HaCaT were significantly suppressed by HH-F and SHH-F, but scarcely by LF-H. The cytoprotective effects of SHH-F had a tendency to be superior to that of HH-F. And the cytoprotective effects of SHH-F against UVB-induced injuries were more effective than those of UVA. Irradiation with UVB to HaCaT cells was shown to cause rapid increases in cell-injury-associated symptoms such as intracellular oxidative stress levels, the formation of cyclobutane pyrimidine dimers and chromatin condensation, all of which were repressed by SHH-F. Thus, UVB-induced diverse harmful effects could be prevented by SHH-F, which was suggested to exert the cytoprotective effects through intracellular reactive oxygen species-scavenging in the keratinocytes.
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Hydroxylated fullerene and trehalose sugar are free radical scavenging antioxidants with protective effects against UV-induced cellular damage.
“The synthetic carbon nanoparticle hydroxylated fullerene and trehalose sugar are both free radical scavenging antioxidants with protective effects against UV-induced cellular damage [34,35].”
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- Authors
- Saitoh, Y., Miyanishi, A., Mizuno, H., Kato, S., Aoshima, H., Kokubo, K., & Miwa, N.
- Affiliations
- Prefectural University of Hiroshima (JP)
- The University of Osaka (JP)
- Corresponding author
- Miwa, Nobuhiko
- Venue
- Journal of Photochemistry and Photobiology B Biology
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 102
- Issue
- 1
- Pages
- 69-76
- ISSN
- 1011-1344 (ISSN-L)
- OpenAlex
- W1981863487
- Cited by
- 86
- References
- 68
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 4 · 2014: 9 · 2013: 14 · 2012: 7
- FWCI
- 3.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Photochemistry and Photobiology B Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Fullerene Chemistry and Applications
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- polyhydroxylated fullerenes, fullerenol, keratinocytes, HaCaT keratinocytes, UV-B radiation, UVA, hydroxyl radical scavenging, reactive oxygen species, oxidative stress, DNA damage, cyclobutane pyrimidine dimers, cytoprotection, antioxidants
- MeSH terms
- Chromatin, DNA Damage, Humans, Hydroxylation, Pyrimidine Dimers, Ultraviolet Rays, Keratinocytes, Free Radical Scavengers, Reactive Oxygen Species, Hydroxyl Radical, Cytoprotection, Fullerenes, Intracellular Space
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