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Cited by 33Vitamin D Research StudiesBone health and osteoporosis researchVitamin K Research Studies

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Comparison of free and total 25-hydroxyvitamin D in normal human pregnancy

Tsuprykov, Oleg, Buse, Claudia, Skoblo, Roman, Hocher, Berthold·2019·The Journal of Steroid Biochemistry and Molecular Biology
DOI 10.1016/j.jsbmb.2019.03.008
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Abstract

Vitamin D status correct monitoring during pregnancy is critically important for both maternal and fetal health. 25-Hydroxyvitamin D (25(OH)D) - a prohormone of a biologically active 1,25-dihydroxyvitamin D (1,25(OH) 2 D), despite the lack of biological activity, during the past decades has been routinely used as a main biomarker characterizing vitamin D status. About 85% of 25(OH)D in the bloodstream is bound to its specific carrier - vitamin D-binding protein (DBP), the remaining 15% are loosely bound to albumin, and only less than 0.1% are free in the circulation ("free 25(OH)D"). Total 25(OH)D is the sum of DBP-bound, albumin-bound and free 25(OH)D. According to a "free hormone hypothesis", only free 25(OH)D is able to induce a biological effect. Normal pregnancy is characterized by elevated serum DBP levels, and due to this fact the diagnostic strength of serum total 25(OH)D has been questioned. Free 25(OH)D might be a better characteristic of vitamin D status in this settings. We aimed to compare the diagnostic strength of a routine total 25(OH)D with directly measured free 25(OH)D in normal pregnancy by comparing the association strength between free and total 25(OH)D with biomarkers of bone health (PTH, calcium, bone-specific alkaline phosphatase (BSAP)), lipid metabolism (adiponectin, LDL, HDL), kidney function (urea), endocrine parameters (T4, T3, TSH), and group B water-soluble vitamins. The study was conducted in 368 healthy white pregnant women - residents of nor…

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Research works printed but not linked: 63

  1. Bikle, 2017, Vitamin D metabolites in captivity? Should we measure free or total 25(OH)D to assess vitamin D status?, J. Steroid Biochem. Mol. Biol., 173, 105, 10.1016/j.jsbmb.2017.01.007 · DOI
  2. Tsuprykov, 2018, Why should we measure free 25(OH) vitamin D?, J. Steroid Biochem. Mol. Biol., 180, 87, 10.1016/j.jsbmb.2017.11.014 · DOI
  3. Mendel, 1989, The free hormone hypothesis: a physiologically based mathematical model, Endocr. Rev., 10, 232, 10.1210/edrv-10-3-232 · DOI · Paper page
  4. Heijboer, 2012, Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration, Clin. Chem., 58, 543, 10.1373/clinchem.2011.176545 · DOI
  5. Sempos, 2018, Vitamin D assays and the definition of hypovitaminosis D: results from the First International Conference on Controversies in Vitamin D, Br. J. Clin. Pharmacol., 84, 2194, 10.1111/bcp.13652 · DOI · Paper page
  6. Bikle, 1984, Free 1,25-dihydroxyvitamin D levels in serum from normal subjects, pregnant subjects, and subjects with liver disease, J. Clin. Invest., 74, 1966, 10.1172/JCI111617 · DOI
  7. Heureux, 2017, A direct assay for measuring free 25-hydroxyvitamin D, J. AOAC Int., 100, 1318, 10.5740/jaoacint.17-0084 · DOI
  8. Tsuprykov, 2018, Reference intervals for measured and calculated free 25-hydroxyvitamin D in normal pregnancy, J. Steroid Biochem. Mol. Biol., 181, 80, 10.1016/j.jsbmb.2018.03.005 · DOI
  9. Zhang, 2014, Impact of pregnancy on vitamin D status: a longitudinal study, Br. J. Nutr., 112, 1081, 10.1017/S0007114514001883 · DOI
  10. Ardawi, 1997, Calcium-regulating hormones and parathyroid hormone-related peptide in normal human pregnancy and postpartum: a longitudinal study, Eur. J. Endocrinol., 137, 402, 10.1530/eje.0.1370402 · DOI
  11. Wilson, 1990, Serum free 1,25-dihydroxyvitamin D and the free 1,25-dihydroxyvitamin D index during a longitudinal study of human pregnancy and lactation, Clin. Endocrinol. (Oxf.), 32, 613, 10.1111/j.1365-2265.1990.tb00905.x · DOI
  12. van Hoof, 2001, Relationship between free and total 1,25-dihydroxyvitamin D in conditions of modified binding, Eur. J. Endocrinol., 144, 391, 10.1530/eje.0.1440391 · DOI
  13. Powe, 2010, First trimester vitamin D, vitamin D binding protein, and subsequent preeclampsia, Hypertens. Dallas Tex 1979, 56, 758
  14. Schwartz, 2014, A comparison of measured and calculated free 25(OH) vitamin D levels in clinical populations, J. Clin. Endocrinol. Metab., 99, 1631, 10.1210/jc.2013-3874 · DOI
  15. Schwartz, 2014, Variability in free 25(OH) vitamin D levels in clinical populations, J. Steroid Biochem. Mol. Biol., 144, 156, 10.1016/j.jsbmb.2013.11.006 · DOI
  16. Jones, 2016, Vitamin D expenditure is not altered in pregnancy and lactation despite changes in vitamin D metabolite concentrations, Sci. Rep., 6, 26795, 10.1038/srep26795 · DOI
  17. Kim, 2017, Clinical utility of measurement of vitamin D-binding protein and calculation of bioavailable vitamin D in assessment of vitamin D status, Ann. Lab. Med., 37, 34, 10.3343/alm.2017.37.1.34 · DOI
  18. Best, 2018, Longitudinal changes in serum vitamin D binding protein and free 25-hydroxyvitamin D in a multiracial cohort of pregnant adolescents, J. Steroid Biochem. Mol. Biol., 186, 79, 10.1016/j.jsbmb.2018.09.019 · DOI
  19. Gustafsson, 2018, Alterations in the vitamin D endocrine system during pregnancy: a longitudinal study of 855 healthy Norwegian women, PLoS One, 13, 10.1371/journal.pone.0195041 · DOI
  20. Alzaman, 2016, Vitamin D status of black and white Americans and changes in vitamin D metabolites after varied doses of vitamin D supplementation, Am. J. Clin. Nutr., 104, 205, 10.3945/ajcn.115.129478 · DOI
  21. Johnsen, 2014, Serum free and bio-available 25-hydroxyvitamin D correlate better with bone density than serum total 25-hydroxyvitamin D, Scand. J. Clin. Lab. Invest., 74, 177, 10.3109/00365513.2013.869701 · DOI · Paper page
  22. Pop, 2015, Vitamin D-binding protein in healthy pre- and postmenopausal women: relationship with estradiol concentrations, Endocr. Pract., 21, 936, 10.4158/EP15623.OR · DOI
  23. Carlsson, 2018, Directly measured free 25-hydroxy vitamin D levels show no evidence of vitamin D deficiency in young Swedish women with anorexia nervosa, Eat. Weight Disord. EWD, 23, 247, 10.1007/s40519-017-0392-y · DOI
  24. Toldy, 2018, Prognostic relevance of circulating 25OHD fractions for early recovery and survival in patients with hip fracture, J. Clin. Med., 7, 10.3390/jcm7080193 · DOI
  25. McKee, 2018, Screening for vitamin D deficiency in Black Americans: comparison of total, free, bioavailable 25 hydroxy vitamin D levels with parathyroid hormone levels and bone mineral density, J. Nutr. Health Aging, 22, 1045, 10.1007/s12603-018-1080-y · DOI
  26. Denburg, 2013, Vitamin D bioavailability and catabolism in pediatric chronic kidney disease, Pediatr. Nephrol. Berl. Ger., 28, 1843, 10.1007/s00467-013-2493-9 · DOI
  27. Oleröd, 2017, The variation in free 25-hydroxy vitamin D and vitamin D-binding protein with season and vitamin D status, Endocr. Connect., 6, 111, 10.1530/EC-16-0078 · DOI
  28. Goswami, 2017, Vitamin D-binding protein, vitamin D status and serum bioavailable 25(OH)D of young Asian Indian males working in outdoor and indoor environments, J. Bone Miner. Metab., 35, 177, 10.1007/s00774-016-0739-x · DOI
  29. Jemielita, 2016, Association of 25-hydroxyvitamin D with areal and volumetric measures of bone mineral density and parathyroid hormone: impact of vitamin D-binding protein and its assays, Osteoporos. Int., 27, 617, 10.1007/s00198-015-3296-6 · DOI
  30. Dastani, 2014, In healthy adults, biological activity of vitamin D, as assessed by serum PTH, is largely independent of DBP concentrations, J. Bone Miner. Res., 29, 494, 10.1002/jbmr.2042 · DOI
  31. Sollid, 2016, Effects of vitamin D binding protein phenotypes and vitamin D supplementation on serum total 25(OH)D and directly measured free 25(OH)D, Eur. J. Endocrinol., 174, 445, 10.1530/EJE-15-1089 · DOI
  32. Aloia, 2015, Free 25(OH)D and calcium absorption, PTH, and markers of bone turnover, J. Clin. Endocrinol. Metab., 100, 4140, 10.1210/jc.2015-2548 · DOI
  33. Wang, 2017, Free and bioavailable 25-hydroxyvitamin D levels in patients with primary hyperparathyroidism, Endocr. Pract., 23, 66, 10.4158/EP161434.OR · DOI
  34. Mantecón, 2018, Marker of vitamin D status in healthy children: free or total 25-hydroxyvitamin D?, PLoS One, 13, e0202237, 10.1371/journal.pone.0202237 · DOI
  35. Gopal-Kothandapani, 2019, Effect of vitamin D supplementation on free and total vitamin D: a comparison of Asians and Caucasians, Clin. Endocrinol. (Oxf.), 90, 222, 10.1111/cen.13825 · DOI
  36. Leaf, 2013, Dysregulated mineral metabolism in patients with acute kidney injury and risk of adverse outcomes, Clin. Endocrinol. (Oxf.), 79, 491, 10.1111/cen.12172 · DOI
  37. Aloia, 2015, Free 25(OH)D and the vitamin D paradox in african americans, J. Clin. Endocrinol. Metab., 100, 3356, 10.1210/JC.2015-2066 · DOI
  38. Ashraf, 2014, Associations between vascular health indices and serum total, free and bioavailable 25-hydroxyvitamin D in adolescents, PLoS One, 9, e114689, 10.1371/journal.pone.0114689 · DOI
  39. Aggarwal, 2016, Bioavailable vitamin D levels are reduced and correlate with bone mineral density and markers of mineral metabolism in adults with nephrotic syndrome, Nephrol. Carlton Vic., 21, 483, 10.1111/nep.12638 · DOI
  40. Lopez-Molina, 2018, Measured free 25-hydroxyvitamin D in healthy children and relationship to total 25-hydroxyvitamin D, calculated free 25-hydroxyvitamin D and vitamin D binding protein, Clin. Biochem., 61, 23, 10.1016/j.clinbiochem.2018.08.007 · DOI
  41. Bhan, 2012, Bioavailable vitamin D is more tightly linked to mineral metabolism than total vitamin D in incident hemodialysis patients, Kidney Int., 82, 84, 10.1038/ki.2012.19 · DOI
  42. Schwartz, 2016, Response of vitamin D concentration to vitamin D3 administration in older adults without sun exposure: a randomized double-blind trial, J. Am. Geriatr. Soc., 64, 65, 10.1111/jgs.13774 · DOI
  43. Shieh, 2016, Effects of high-dose vitamin D2 versus D3 on total and free 25-hydroxyvitamin D and markers of calcium balance, J. Clin. Endocrinol. Metab., 101, 3070, 10.1210/jc.2016-1871 · DOI
  44. Lundgren, 1997, Tissue distribution of human gp330/megalin, a putative Ca(2+)-sensing protein, J. Histochem. Cytochem., 45, 383, 10.1177/002215549704500306 · DOI
  45. Geraghty, 2017, Maternal and fetal blood lipid concentrations during pregnancy differ by maternal body mass index: findings from the ROLO study, BMC Pregnancy Childbirth, 17, 360, 10.1186/s12884-017-1543-x · DOI
  46. Bartels, 2011, Cholesterol in pregnancy: a review of knowns and unknowns, Obstet. Med., 4, 147, 10.1258/om.2011.110003 · DOI
  47. Jorde, 2018, Exploring the association between serum 25-hydroxyvitamin D and serum lipids-more than confounding?, Eur. J. Clin. Nutr., 72, 526, 10.1038/s41430-018-0088-z · DOI
  48. Glueck, 2016, Associations between serum 25-hydroxyvitamin D and lipids, lipoprotein cholesterols, and homocysteine, N. Am. J. Med. Sci., 8, 284, 10.4103/1947-2714.187137 · DOI
  49. Makgoba, 2011, First-trimester circulating 25-hydroxyvitamin D levels and development of gestational diabetes mellitus, Diabetes Care, 34, 1091, 10.2337/dc10-2264 · DOI
  50. Al-Musharaf, 2018, Vitamin D deficiency prevalence and predictors in early pregnancy among arab women, Nutrients, 10, 10.3390/nu10040489 · DOI
  51. Kane, 2013, Vitamin D3 effects on lipids differ in statin and non-statin-treated humans: superiority of free 25-OH D levels in detecting relationships, J. Clin. Endocrinol. Metab., 98, 4400, 10.1210/jc.2013-1922 · DOI
  52. Catalano, 2006, Adiponectin in human pregnancy: implications for regulation of glucose and lipid metabolism, Diabetologia, 49, 1677, 10.1007/s00125-006-0264-x · DOI
  53. Achari, 2017, Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction, Int. J. Mol. Sci., 18, 10.3390/ijms18061321 · DOI
  54. Gannagé-Yared, 2009, Vitamin D in relation to metabolic risk factors, insulin sensitivity and adiponectin in a young Middle-Eastern population, Eur. J. Endocrinol., 160, 965, 10.1530/EJE-08-0952 · DOI
  55. Nimitphong, 2009, The association between vitamin D status and circulating adiponectin independent of adiposity in subjects with abnormal glucose tolerance, Endocrine, 36, 205, 10.1007/s12020-009-9216-9 · DOI
  56. Bidulescu, 2014, Association between vitamin D and adiponectin and its relationship with body mass index: the META-health study, Front. Publ. Health, 2, 193, 10.3389/fpubh.2014.00193 · DOI
  57. Vaidya, 2012, The independent association between 25-hydroxyvitamin D and adiponectin and its relation with BMI in two large cohorts: the NHS and the HPFS, Obes. Silver Spring Md., 20, 186, 10.1038/oby.2011.210 · DOI
  58. Mazaki-Tovi, 2007, Maternal serum adiponectin levels during human pregnancy, J. Perinatol., 27, 77, 10.1038/sj.jp.7211639 · DOI
  59. Mousa, 2017, Relationship between vitamin D and gestational diabetes in overweight or obese pregnant women may be mediated by adiponectin, Mol. Nutr. Food Res., 61, 10.1002/mnfr.201700488 · DOI · Paper page
  60. Sande, 2013, Vitamin B12 in pregnancy: maternal and fetal/neonatal effects—a review, Open J. Obstet. Gynecol., 3, 599, 10.4236/ojog.2013.37107 · DOI
  61. Percudani, 2009, The B6 database: a tool for the description and classification of vitamin B6-dependent enzymatic activities and of the corresponding protein families, BMC Bioinformatics, 10, 273, 10.1186/1471-2105-10-273 · DOI
  62. Dror, 2012, Interventions with vitamins B6, B12 and C in pregnancy, Paediatr. Perinat. Epidemiol., 26, 55, 10.1111/j.1365-3016.2012.01277.x · DOI
  63. Cheung, 2013, Renal physiology of pregnancy, Adv. Chronic Kidney Dis., 20, 209, 10.1053/j.ackd.2013.01.012 · DOI

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Metadata

Authors
Tsuprykov, O., Buse, C., Skoblo, R., & Hocher, B.
Affiliations
  • NanoTecCenter Weiz Forschungsgesellschaft (Austria) (AT)
  • Hunan Normal University (CN)
Corresponding author
Hocher, Berthold
ORCID
Venue
The Journal of Steroid Biochemistry and Molecular Biology
Publisher
Elsevier BV
Published
2019-06
Volume
190
Pages
29-36
ISSN
0960-0760 (ISSN-L)
DOI
10.1016/j.jsbmb.2019.03.008
OpenAlex
W2922539926
Cited by
33
References
0
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 6 · 2020: 6 · 2019: 3
FWCI
3.52
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Vitamin D Research Studies
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin K Research Studies
Keywords
free 25-hydroxyvitamin D, vitamin D status, pregnancy, vitamin D binding protein, free hormone hypothesis, bone metabolism, bone-specific alkaline phosphatase, parathyroid hormone, adiponectin, lipid metabolism, thyroid hormones, water-soluble vitamins, gestational age
MeSH terms
Adult, Female, Gestational Age, Humans, Pregnancy, Protein Binding, Vitamin D, Vitamin D-Binding Protein, Vitamins, Biomarkers, Young Adult

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Tsuprykov, O., Buse, C., Skoblo, R., & Hocher, B. (2019). Comparison of free and total 25-hydroxyvitamin D in normal human pregnancy. The Journal of Steroid Biochemistry and Molecular Biology. https://doi.org/10.1016/j.jsbmb.2019.03.008