Expression Profiling of Major Heat Shock Protein Genes during Different Seasons in Cattle (Bos Indicus) and Buffalo (Bubalus Bubalis) under Tropical Climatic Condition
Abstract
Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expression pattern in Sahiwal and Tharparkar breeds of zebu cattle (Bos indicus) and Murrah buffalo (Bubalus bubalis) with respect to different seasons. Quantitative real time polymerase chain reaction was performed to analyze the transcript variants of three HSP70 family genes (HSPA1A, HSPA1B, and HSPA8) and HSP10, HSP60, HSP90 and HSF1 in each breed. The major finding of this study was the higher abundance of all the studied HSP genes during summer and winter compared to spring season, but the magnitude of increase was higher during summer as compared to winter. HSPA1A and HSPA1B genes showed maximal induction (P<0.001) during summer and winter while HSP60 and HSP10 were found to be the second most abundantly expressed HSPs. The relative mRNA abundance of HSF1 significantly increased (P<0.001) in Murrah buffalo compared to Tharparkar and Sahiwal cattle during summer and winter. Expression pattern of heat shock protein genes indicated that amongst the breeds, the expression was higher in Murrah buffalo compared to Sahiwal and Tharparkar cattle, thereby indicating the more adaptive capacity of later during periods of stress. Hence, this study suggests that heat shock protein genes may be conveniently used as biomarkers for assessing stress response in cattle and buffalo and the expression is species and breed-specific. Furthermore, the variation in expression is associated with heat tolerance and adaptation to different climatic conditions.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HSPA1A and HSPA1B expression vary seasonally in zebu cattle, being higher in summer than in spring.
“HSPA1A and HSPA1B expression vary seasonally in zebu cattle, being higher in summer than in spring [114].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [114]
Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · see citing works
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP
Felsenstein, J. (1985) · Evolution · doi:10.1111/j.1558-5646.1985.tb00420.x · Cited by 44,735 · see citing works
MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013) · Molecular biology and evolution · doi:10.1093/molbev/mst197 · Cited by 41,174 · see citing works
Confidence Limits on Phylogenies: An Approach Using the Bootstrap
Felsenstein, J. (1985) · Evolution · doi:10.2307/2408678 · Cited by 19,371 · see citing works
Lindquist, S. L. (1986) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.55.070186.005443 · Cited by 4,611 · see citing works
The evolutionary and ecological role of heat shock proteins
Sørensen, J. G., Kristensen, T. N., & Loeschcke, V. (2003) · Ecology Letters · doi:10.1046/j.1461-0248.2003.00528.x · Cited by 1,398 · see citing works
Effects of climate changes on animal production and sustainability of livestock systems
Nardone, A., Ronchi, B., Lacetera, N., Ranieri, M., & Bernabucci, U. (2010) · Livestock Science · doi:10.1016/j.livsci.2010.02.011 · Cited by 1,274 · see citing works
Major Advances Associated with Environmental Effects on Dairy Cattle
Collier, R. J., Dahl, G. E., & VanBaale, M. J. (2006) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(06)72193-2 · Cited by 681 · see citing works
Physiological and cellular adaptations of zebu cattle to thermal stress
Hansen, P. J. (2004) · Animal Reproduction Science · doi:10.1016/j.anireprosci.2004.04.011 · Cited by 642 · see citing works
Invited Review: Genes Involved in the Bovine Heat Stress Response
Collier, R. J., Collier, J. L., Rhoads, R. P., & Baumgard, L. H. (2008) · Journal of Dairy Science · doi:10.3168/jds.2007-0540 · Cited by 454 · see citing works
Nutritional strategies for managing the heat-stressed dairy cow
West, J. W. (1997) · Journal of Animal Science · doi:10.2527/1997.77suppl_221x · Cited by 285 · see citing works
Lin, K. M.-C., Lin, B., Lian, I., Mestril, R., Scheffler, I. E., & DILLMANN, W. H. (2001) · Circulation · doi:10.1161/01.cir.103.13.1787 · Cited by 282 · see citing works
Fargnoli, J., Kunisada, T., Fornace, A. J., Schneider, E. L., & Holbrook, N. J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.2.846 · Cited by 223 · see citing works
Lacetera, N., Bernabucci, U., Scalia, D., Basiricò, L., Morera, P., & Nardone, A. (2006) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(06)72510-3 · Cited by 193 · see citing works
Pfister, G., Stroh, C. M., Perschinka, H., Kind, M., Knoflach, M., Hinterdorfer, P., & Wick, G. (2005) · Journal of Cell Science · doi:10.1242/jcs.02292 · Cited by 187 · see citing works
Heads, R. J., Yellon, D. M., & Latchman, D. S. (1995) · Journal of Molecular and Cellular Cardiology · doi:10.1016/s0022-2828(95)90722-x · Cited by 147 · see citing works
Expression profile of HSP genes during different seasons in goats (Capra hircus)
Dangi, S. S., Gupta, M. K., Maurya, D., Yadav, V. P., Panda, R. P., Singh, G., Mohan, N. H., Bhure, S. K., Das, B. C., Bag, S., et al. (2012) · Tropical Animal Health and Production · doi:10.1007/s11250-012-0155-8 · Cited by 131 · see citing works
Down-regulation of IL-1 beta biosynthesis by inducers of the heat-shock response.
Schmidt, J. A., & Abdulla, E. M. (1988) · The Journal of Immunology · doi:10.4049/jimmunol.141.6.2027 · Cited by 125 · see citing works
Seasonal variation in expression pattern of genes under HSP70
Banerjee, D. K., Upadhyay, R. C., Chaudhary, U. B., Kumar, R., Singh, S., Ashutosh, A., Jagan Mohanarao, G., Polley, S., Mukherjee, A., Das, T. K., et al. (2013) · Cell Stress and Chaperones · doi:10.1007/s12192-013-0469-0 · Cited by 121 · see citing works
Self-citationResponses of bovine lymphocytes to heat shock as modified by breed and antioxidant status
Kamwanja, L. A., Chase, C. C., Gutierrez, J. A., et al. (1994) · Journal of Animal Science · doi:10.2527/1994.722438x · Cited by 121 · see citing works
HALL, D. M., Oberley, T. D., Moseley, P. L., Buettner, G. R., Oberley, L. W., Weindruch, R. H., & Kregel, K. C. (2000) · The FASEB Journal · doi:10.1096/fasebj.14.1.78 · Cited by 107 · see citing works
Dangi, S. S., Gupta, M. K., Nagar, V., Yadav, V. P., Dangi, S. K., Shankar, O., Chouhan, V. S., Kumar, P., Singh, G., & Sarkar, M. (2014) · International Journal of Biometeorology · doi:10.1007/s00484-014-0809-5 · Cited by 92 · see citing works
King, Y.-T., Lin, C., Lin, J., & Lee, W.-C. (2002) · Journal of Experimental Biology · doi:10.1242/jeb.205.2.273 · Cited by 91 · see citing works
Heat stress in Murrah buffalo calves
Das, S., Upadhyay, R. C., & Madan, M. L. (1999) · Livestock Production Science · doi:10.1016/s0301-6226(99)00040-8 · Cited by 85 · see citing works
Self-citationInfluence of induced heat stress on HSP70 in buffalo lymphocytes
Mishra, A., Hooda, O. K., Singh, G., et al. (2010) · Journal of Animal Physiology and Animal Nutrition · doi:10.1111/j.1439-0396.2010.01082.x · Cited by 72 · see citing works
Synthesis of heat stress proteins in lymphocytes from livestock.
Guerriero, V., & Raynes, D. A. (1990) · Journal of Animal Science · doi:10.2527/1990.6892779x · Cited by 71 · see citing works
Gali, J. M., Mukherjee, A., Banerjee, D. K., Gohain, M., Dass, G., Brahma, B., Datta, T. K., Upadhyay, R. C., & De, S. (2013) · Small Ruminant Research · doi:10.1016/j.smallrumres.2013.10.014 · Cited by 64 · see citing works
Self-citationSingh, A. K., Upadhyay, R. C., Malakar, D., Kumar, S., & Singh, S. (2014) · Journal of Thermal Biology · doi:10.1016/j.jtherbio.2014.04.006 · Cited by 57 · see citing works
Self-citationZULKIFLI, I., Norbaiyah, B., Cheah, Y. W., et al. (2010) · animal · doi:10.1017/s1751731110000285 · Cited by 50 · see citing works
Purification, characterization and expression kinetics of heat shock protein 70 from Bubalus bubalis
Patir, H., & Upadhyay, R. C. (2009) · Research in Veterinary Science · doi:10.1016/j.rvsc.2009.09.004 · Cited by 47 · see citing works
Self-citationMorange, M. (2005) · Springer eBooks · doi:10.1007/3-540-29717-0_7 · Cited by 44 · see citing works
XiangHong, J., Yong, Y., Han-jin, X., et al. (2011) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2011.07.004 · Cited by 43 · see citing works
Kishore, A., Sodhi, M., Khate, K., et al. (2013) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2013.02.003 · Cited by 36 · see citing works
Yadav, A. K., Singh, K., Singh, M. K., Saini, N., Palta, P., Manik, R., Singla, S., Upadhyay, R. C., & Chauhan, M. (2013) · Reproduction in Domestic Animals · doi:10.1111/rda.12175 · Cited by 35 · see citing works
Self-citationCiavarra, R. P., & Simeone, A. (1990) · Cellular Immunology · doi:10.1016/0008-8749(90)90212-a · Cited by 30 · see citing works
Functional Organization of hsp70 Cluster in Camel (Camelus dromedarius) and Other Mammals
Garbuz, D. G., Астахова, Л. Н., Zatsepina, O. G., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0027205 · Cited by 27 · see citing works
Age dependence of heat stress mediated cardioprotection
Gray, C. C., Amrani, M., Smolenski, R. T., et al. (2000) · The Annals of Thoracic Surgery · doi:10.1016/s0003-4975(00)01445-4 · Cited by 25 · see citing works
Ashraf, S., Shah, S. M. A., Saini, N., et al. (2014) · Journal of Assisted Reproduction and Genetics · doi:10.1007/s10815-014-0275-3 · Cited by 22 · see citing works
Self-citationYan-Xin, L. I. U., Li, D., Cui, Q.-W., et al. (2011) · Hereditas (Beijing) · doi:10.3724/sp.j.1005.2010.00935 · Cited by 12 · see citing works
The gene encoding PBP74/CSA/motalin-1, a novel mouse hsp70, maps to mouse chromosome 18.
Ohashi, M., Oyanagi, M., Hatakeyama, K., et al. (1995) · PubMed · Cited by 9 · see citing works
Rada, A., Merentes, E., Rodríguez, M., et al. (2010) · PubMed · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Saravanan, K. A., Panigrahi, M., Kumar, H., Parida, S., Bhushan, B., Gaur, G. K., Dutt, T., Mishra, B. P., & Singh, R. K. (2021) · Genomics · doi:10.1016/j.ygeno.2021.02.009 · Cited by 98 · see citing works
Igoshin, A. V., Yurchenko, A. A., Belonogova, N. M., Petrovsky, D. V., Aitnazarov, R. B., Soloshenko, V. A., Yudin, N. S., & Larkin, D. M. (2019) · BMC Genetics · doi:10.1186/s12863-019-0725-0 · Cited by 42 · see citing works
The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution
Liu, Y., Dao, W., Gao, R., Fan, X., Yang, R., & Miao, Y. (2026) · Animals · doi:10.3390/ani16050776 · Cited by 1 · see citing works
- Authors
- Kumar, A., Ashraf, S., Goud, T. S., Grewal, A., Singh, S. V., Yadav, B. R., & Upadhyay, R. C.
- Affiliations
- Indian Council of Agricultural Research (IN)
- National Dairy Research Institute (IN)
- Kurukshetra University (IN)
- Corresponding author
- Kumar, Anil
- Venue
- Journal of Thermal Biology
- Publisher
- Elsevier BV
- Published
- 2015-07
- Volume
- 51
- Pages
- 55-64
- ISSN
- 0306-4565 (ISSN-L)
- OpenAlex
- W2010093175
- Cited by
- 104
- References
- 41
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 9 · 2023: 14 · 2022: 10 · 2021: 16 · 2020: 13 · 2019: 11 · 2018: 7 · 2017: 5 · 2016: 6 · 2015: 2
- FWCI
- 9.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Thermal Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Initiative on Climate Resilient Agriculture (2049/3033)
- Indian Council of Agricultural Research
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Effects of Environmental Stressors on Livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
- Keywords
- heat shock proteins, seasonal gene expression, HSP70 family, HSPA1A, HSPA1B, Hsc70, HSP60, Hsp10, HSP90, HSF1, Bos indicus, Murrah buffalo, Sahiwal, Tharparkar, breed differences, heat tolerance, stress biomarkers, tropical adaptation
- MeSH terms
- Acclimatization, Animals, Buffaloes, Cattle, Heat-Shock Proteins, RNA, Messenger, Seasons, Tropical Climate, Heat-Shock Response, Transcriptome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review