Effects of mixed cultures of Saccharomyces cerevisiae and Lactobacillus plantarum in alcoholic fermentation on the physicochemical and sensory properties of citrus vinegar
Abstract
Alcoholic fermentation is an essential step in producing high-quality vinegar and typically involves the use of pure yeast to initiate fermentation. In this study, two kinds of citrus vinegars were prepared with a pure culture of Saccharomyces cerevisiae and mixed cultures of Saccharomyces cerevisiae and Lactobacillus plantarum, respectively. During the alcoholic-fermentation stage, the contents of formic, lactic, and total organic acids were higher in the mixed cultures than in the pure culture. Mixed cultures citrus vinegar (MCCV) showed higher antioxidant activity than pure culture citrus vinegar (PCCV). The contents of sweet and umami free amino acids in MCCV were higher than those in PCCV. Meanwhile, flavor groups such as esters, alcohols, and aldehydes in MCCV also significantly improved. According to sensory analysis, MCCV showed higher intensity for sweet and umami attributes, as well as flowery and fruity attributes. Thus, mixed cultures of Saccharomyces cerevisiae and Lactobacillus plantarum in alcoholic fermentation can effectively improve the flavor and quality of citrus vinegar, thereby indicating additional economic benefits of fermentation.
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Benzyl alcohol possesses a sweet, rose-like, and honey-like aroma, produced through deamination, hydroxylation, and reduction of phenylalanine.
“It possesses a sweet, rose-like, and honey-like aroma, which is produced through the deamination, hydroxylation, and reduction of phenylalanine [36,94].”
From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [36]
Benzyl alcohol typically exhibits the highest odor activity value among alcohols in vinegar.
“Benzyl alcohol typically exhibits the highest odor activity value among the alcohols in vinegar.”
From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [36]
Phenylacetaldehyde is the most prominent aroma component in citrus vinegar.
“Phenylacetaldehyde, in particular, is the most prominent aroma component in citrus vinegar [36].”
From The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development (2025) · cites this paper as [36]
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Self-citation
- Authors
- Chen, Y., Huang, Y., Bai, Y., Fu, C., Zhou, M., Gao, B., Wang, C., Li, D., Hu, Y., & Xu, N.
- Affiliations
- Hubei University of Technology (CN)
- Corresponding author
- Hu, Yong
- Xu, Ning
- Venue
- LWT
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 84
- Pages
- 753-763
- ISSN
- 0023-6438 (ISSN-L)
- OpenAlex
- W2624945369
- Cited by
- 91
- References
- 59
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 15 · 2023: 10 · 2022: 12 · 2021: 12 · 2020: 7 · 2019: 5 · 2018: 6 · 2017: 1
- FWCI
- 5.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- LWT (publishedVersion)
- Funders
- Natural Science Foundation of Hubei Province (2015CFB678, 2015CFB679)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- Saccharomyces cerevisiae, Lactobacillus plantarum, mixed culture, alcoholic fermentation, vinegar flavor, free amino acids, organic acids, antioxidant activity, sensory properties, vinegar production
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