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Cited by 2Bacterial Genetics and BiotechnologyMicrobial metabolism and enzyme functionMicrobial Metabolic Engineering and Bioproduction

The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.

The interplay between glucose and aromatic compound regulation by two IclR-type transcription factors, LigR1 and LigR2, in Pseudomonas putida KT2440

Open access
Kadriu, Elina, Qin, Sophie, Prezioso, Stephanie M., Christendat, Dinesh·2026·Microbiological Research
DOI 10.1016/j.micres.2025.128382

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Abstract

Carbon utilization strategies are fundamental to microbial proliferation within complex ecosystems like the soil microbiome. These strategies dictate how microbes prioritize, and metabolize available carbon compounds, shaping community dynamics and ecological outcomes. Pseudomonas putida KT2440, a soil bacterium renowned for its metabolic versatility, exemplifies this adaptive capacity. However, the regulatory mechanism it employs to prioritize sugars vs aromatic compounds for their energy requirement remains poorly understood. Here, we investigated two IclR-type transcriptional regulators, LigR1 and LigR2, which control expression of the lig1 and lig2 operons. Functional analyses reveal that LigR1 and LigR2 activate lig1 but repress the lig2 operon. 4-hydroxybenzoate binding to LigR1 represses gene expression, whereas quinate, protocatechuate, and 4-hydroxybenzoate bind to LigR2 to induce lig2 operon expression. Additionally, ligR1 deletion causes growth defects on glucose and 4-hydroxybenzoate, accompanied by cell elongation and aggregation. We propose that the lig1 operon mediates dual influx of glucose and aromatics via its major facilitator superfamily transporter, while the lig2 operon catalyzes aromatic breakdown through a protocatechuate intermediate and meta-cleavage pathway, supplying oxaloacetate to the TCA cycle. Importantly, P. putida prioritizes shikimate pathway intermediates as energy sources under specific metabolic conditions, such as their accumulation. Overall, these findings redefine the metabolic flexibility of soil pseudomonads and reveal a novel mechanism for thriving in chemically diverse environments. By illuminating a dual regulatory system, our study offers new insight into microbial carbon flux and on the traditional biosynthetic paradigm of the shikimate pathway, revealing its unexpected role in supplying the organism with energy generating compounds.

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Metadata

Authors
Kadriu, E., Qin, S., Prezioso, S. M., & Christendat, D.
Affiliations
University of Toronto (CA)
Corresponding author
Christendat, Dinesh
ORCID
Venue
Microbiological Research
Publisher
Elsevier BV
Published
2026-02
Volume
303
Pages
128382
ISSN
0944-5013 (ISSN-L)
DOI
10.1016/j.micres.2025.128382
OpenAlex
W4415641608
Cited by
2
References
82
Citations by year
2026: 2
FWCI
1.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiological Research (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
Funders
Natural Sciences and Engineering Research Council of Canada (RGPIN-2020-06052)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Pseudomonas putida KT2440, aromatic compound metabolism, glucose metabolism, p-hydroxybenzoic acid, quinic acid, protocatechuic acid, shikimate pathway, major facilitator superfamily transporter, protocatechuate pathway, meta-cleavage pathway, TCA cycle, oxaloacetate, soil bacteria, metabolic flexibility, gene expression regulation
MeSH terms
Bacterial Proteins, Glucose, Operon, Parabens, Transcription Factors, Gene Expression Regulation, Bacterial, Pseudomonas putida, Hydroxybenzoates

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