Functional characterization of LIT1, the Leishmania amazonensis ferrous iron transporter
Open accessAbstract
Leishmania amazonensis LIT1 was identified based on homology with IRT1, a ferrous iron transporter from Arabidopsis thaliana. Deltalit1L. amazonensis are defective in intracellular replication and lesion formation in vivo, a virulence phenotype attributed to defective intracellular iron acquisition. Here we functionally characterize LIT1, directly demonstrating that it functions as a ferrous iron membrane transporter from the ZIP family. Conserved residues in the predicted transmembrane domains II, IV, V and VII of LIT1 are essential for iron transport in yeast, including histidines that were proposed to function as metal ligands in ZIP transporters. LIT1 also contains two regions within the predicted intracellular loop that are not found in Arabidopsis IRT1. Deletion of region I inhibited LIT1 expression on the surface of Leishmania promastigotes. Deletion of region II did not interfere with LIT1 trafficking to the surface, but abolished its iron transport capacity when expressed in yeast. Mutagenesis revealed two motifs within region II, HGHQH and TPPRDM, that are independently required for iron transport by LIT1. D263 was identified as a key residue required for iron transport within the TPPRDM motif, while P260 and P261 were dispensable. Deletion of proline-rich regions within region I and between regions I and II did not affect iron transport in yeast, but in L. amazonensis were not able to rescue the intracellular growth of Deltalit1 parasites, or their ability to form lesions in mice. These results are consistent with a potential role of the unique intracellular loop of LIT1 in intracellular regulation by Leishmania-specific factors.
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A transferrin receptor-based mechanism for Fe uptake in Leishmania was not confirmed by subsequent studies.
“In Leishmania, a transferrin receptor-based mechanism for Fe uptake was also initially postulated, but this mechanism was not confirmed by subsequent studies [87].”
From Strategies of intracellular pathogens for obtaining iron from the environment (2015) · cites this paper as [87]
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Aghabi, D., Rubio, C. G., Martinez, M. C., Pouzache, A., Gibson, E. J., Pagura, L., & Harding, C. R. (2025) · eLife · doi:10.7554/elife.108666.1
ZFT is the major iron and zinc transporter in Toxoplasma gondiiPreprint
Aghabi, D., Rubio, C. G., Martinez, M. C., Pouzache, A., Gibson, E. J., Pagura, L., Fairweather, S. J., van Dooren, G. G., & Harding, C. R. (2026) · eLife · doi:10.7554/elife.108666.2
- Authors
- Jacques, I., Andrews, N. W., & Huynh, C.
- Affiliations
- Yale University (US)
- Corresponding author
- Andrews, Norma W.
- ORCID
- Andrews, Norma W.
- Venue
- Molecular and Biochemical Parasitology
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 170
- Issue
- 1
- Pages
- 28-36
- ISSN
- 0166-6851 (ISSN-L)
- PMC
- PMC2815141
- OpenAlex
- W2163103200
- Cited by
- 42
- References
- 23
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Biochemical Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (R37 AI034867S1, R01AI067979, R37AI034867)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- LIT1, Leishmania amazonensis, ZIP family, virulence, promastigotes, transmembrane domain mutations, intracellular loop, intracellular replication
- MeSH terms
- Iron, Leishmania mexicana, Protozoan Proteins, Protein Structure, Tertiary, Cation Transport Proteins
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