Cited by 1,679Plant Micronutrient Interactions and EffectsIron Metabolism and DisordersPlant nutrient uptake and metabolism

IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth

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Vert, Grégory, Grotz, Natasha, Dédaldéchamp, Fabienne, Gaymard, Frédéric, Guerinot, Mary Lou, Briat, Jean-François, Curie, Catherine·2002·The Plant Cell

Abstract

Plants are the principal source of iron in most diets, yet iron availability often limits plant growth. In response to iron deficiency, Arabidopsis roots induce the expression of the divalent cation transporter IRT1. Here, we present genetic evidence that IRT1 is essential for the uptake of iron from the soil. An Arabidopsis knockout mutant in IRT1 is chlorotic and has a severe growth defect in soil, leading to death. This defect is rescued by the exogenous application of iron. The mutant plants do not take up iron and fail to accumulate other divalent cations in low-iron conditions. IRT1-green fluorescent protein fusion, transiently expressed in culture cells, localized to the plasma membrane. We also show, through promoter::beta-glucuronidase analysis and in situ hybridization, that IRT1 is expressed in the external cell layers of the root, specifically in response to iron starvation. These results clearly demonstrate that IRT1 is the major transporter responsible for high-affinity metal uptake under iron deficiency.

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Crossref · 10.1105/tpc.001388

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  • Proteins such as ZRT- and IRT-like proteins, YSL transporters, NRAMP, channels, and other genes related to cadmium uptake/restriction play critical roles in metal cation influx to the symplasm.

    “For influx to the symplasm, a lot of proteins such as ZRT- and IRT-like proteins, stripe-Like (YSL) transporters, natural resistance-associated macrophage proteins (NRAMP), channels and other genes related to Cd-uptake/restriction play critical roles [32].”

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Authors
Vert, G., Grotz, N., Dédaldéchamp, F., Gaymard, F., Guerinot, M. L., Briat, J.-F., & Curie, C.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Dartmouth College (US)
Venue
The Plant Cell
Published
2002-06
Volume
14
Issue
6
Pages
1223-1233
ISSN
  • 1040-4651 (ISSN-L)
  • 1532-298X
OpenAlex
W2124946184
Cited by
1,679
References
42
Citations by year
2026: 89 · 2025: 80 · 2024: 81 · 2023: 100 · 2022: 121 · 2021: 113 · 2020: 98 · 2019: 92 · 2018: 82 · 2017: 78 · 2016: 75 · 2015: 73 · 2014: 77 · 2013: 66 · 2012: 73
FWCI
37.16
Type
journal article
Language
en
Open access
Locations
  • The Plant Cell (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Keywords
IRT1, iron absorption, iron deficiency, Arabidopsis thaliana, plasma membrane localization, chlorosis, knockout mutants, iron starvation response
MeSH terms
Base Sequence, Biological Transport, Cations, Divalent, Genes, Lethal, Homeostasis, Iron, Luminescent Proteins, NADH, NADPH Oxidoreductases, Plant Proteins, Plasmids, Recombinant Fusion Proteins, Soil, Genes, Plant, Gene Deletion, Arabidopsis, DNA Primers, Plant Roots, Cation Transport Proteins, Arabidopsis Proteins, Plants, Genetically Modified, FMN Reductase, Green Fluorescent Proteins

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