The Conserved Transmembrane Nucleoporin NDC1 Is Required for Nuclear Pore Complex Assembly in Vertebrate Cells
Open accessAbstract
Nuclear pore complexes (NPCs) are large proteinaceous channels embedded in the nuclear envelope (NE), through which exchange of molecules between the nucleus and cytosol occurs. Biogenesis of NPCs is complex and poorly understood. In particular, almost nothing is known about how NPCs are anchored in the NE. Here, we characterize vertebrate NDC1--a transmembrane nucleoporin conserved between yeast and metazoans. We show by RNA interference (RNAi) and biochemical depletion that NDC1 plays an important role in NPC and NE assembly in vivo and in vitro. RNAi experiments suggest a functional link between NDC1 and the soluble nucleoporins Nup93, Nup53, and Nup205. Importantly, NDC1 interacts with Nup53 in vitro. This suggests that NDC1 function involves forming a link between the NE membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
NDC1 is the only human transmembrane Nup conserved across eukaryotes.
“Among the three human transmembrane proteins, NDC1 is the only one that is conserved across eukaryotes (40).”
From AI-based structure prediction empowers integrative structural analysis of human nuclear pores (2022) · cites this paper as [40]
Cites
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Uetz, P., Giot, L., Cagney, G., Mansfield, T. A., Judson, R. S., Knight, J. R., Lockshon, D., Narayan, V., Srinivasan, M., Pochart, P., et al. (2000) · Nature · doi:10.1038/35001009 · Cited by 4,706 · see citing works
A comprehensive two-hybrid analysis to explore the yeast protein interactome
Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., & Sakaki, Y. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.061034498 · Cited by 3,566 · see citing works
Phase separation of integral membrane proteins in Triton X-114 solution.
Bordier, C. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69848-0 · Cited by 3,324 · see citing works
The Yeast Nuclear Pore Complex
Rout, M. P., Aitchison, J. D., Suprapto, A., Hjertaas, K., Zhao, Y., & Chait, B. T. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.148.4.635 · Cited by 1,403 · see citing works
Nuclear Membrane Proteins with Potential Disease Links Found by Subtractive Proteomics
Schirmer, E. C., Florens, L. A., Guan, T., Yates, J. R., & Gerace, L. (2003) · Science · doi:10.1126/science.1088176 · Cited by 654 · see citing works
Identification and characterization of a nuclear pore complex protein
Davis, L. I., & Blobel, G. (1986) · Cell · doi:10.1016/0092-8674(86)90784-1 · Cited by 620 · see citing works
The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly
Walther, T. C., Alves, A., Pickersgill, H., Loïodice, I., Hetzer, M. W., Galy, V., Hülsmann, B. B., Köcher, T., Wilm, M. S., Allen, T., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00235-6 · Cited by 401 · see citing works
Self-citationUfd2, a Novel Autoantigen in Scleroderma, Regulates Sister Chromatid Separation
Spinette, S., Christoph Lengauer, C., Mahoney, J. A., Jallepalli, P. V., Wang, Z., Casciola-Rosen, L., & Rosen, A. (2004) · Cell Cycle · doi:10.4161/cc.3.12.1345 · Cited by 309 · see citing works
Comparative Genomics, Evolution and Origins of the Nuclear Envelope and Nuclear Pore Complex
Mans, B., Anantharaman, V., Aravind, L., & Koonin, E. V. (2004) · Cell Cycle · doi:10.4161/cc.3.12.1316 · Cited by 304 · see citing works
PUSHING THE ENVELOPE: Structure, Function, and Dynamics of the Nuclear Periphery
Hetzer, M. W., Walther, T. C., & Mattaj, I. W. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.090704.151152 · Cited by 298 · see citing works
Self-citationRemoval of a Single Pore Subcomplex Results in Vertebrate Nuclei Devoid of Nuclear Pores
Harel, A., Orjalo, A. V., Vincent, T. M., et al. (2003) · Molecular Cell · doi:10.1016/s1097-2765(03)00116-3 · Cited by 273 · see citing works
Krull, S., Thyberg, J., Björkroth, B., et al. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-03-0165 · Cited by 237 · see citing works
Galy, V., Mattaj, I. W., & Askjaer, P. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-04-0237 · Cited by 186 · see citing works
Self-citationAitchison, J. D., Rout, M. P., Marelli, M., et al. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.131.5.1133 · Cited by 185 · see citing works
Modularity within the architecture of the nuclear pore complex
Schwartz, T. (2005) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2005.03.003 · Cited by 163 · see citing works
Macaulay, C., & Forbes, D. J. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.132.1.5 · Cited by 162 · see citing works
Grandi, P., Dang, T., Pané, N., Shevchenko, A., Mann, M., Forbes, D. J., & Hurt, E. (1997) · Molecular Biology of the Cell · doi:10.1091/mbc.8.10.2017 · Cited by 155 · see citing works
West, R. R., Vaisberg, E., Ding, R., Nurse, P., & McIntosh, J. R. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.10.2839 · Cited by 154 · see citing works
Antonin, W., Franz, C., Haselmann, U., et al. (2005) · Molecular Cell · doi:10.1016/j.molcel.2004.12.010 · Cited by 150 · see citing works
Self-citationNDC1: a nuclear periphery component required for yeast spindle pole body duplication
Winey, M., Hoyt, M. A., Chan, C., et al. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.122.4.743 · Cited by 144 · see citing works
POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope.
Wozniak, R. W., Blobel, G., & Rout, M. P. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.1.31 · Cited by 139 · see citing works
Self-citationChial, H. J., Rout, M. P., Giddings, T. H., et al. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.143.7.1789 · Cited by 133 · see citing works
Hawryluk-Gara, L. A., Shibuya, E. K., & Wozniak, R. W. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-10-0857 · Cited by 131 · see citing works
Self-citationA Link between the Synthesis of Nucleoporins and the Biogenesis of the Nuclear Envelope
Marelli, M., Lusk, C. P., Chan, H., Aitchison, J. D., & Wozniak, R. W. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.4.709 · Cited by 130 · see citing works
Self-citationA gene required for the separation of chromosomes on the spindle apparatus in yeast
Thomas, J. H., & Botstein, D. (1986) · Cell · doi:10.1016/0092-8674(86)90485-x · Cited by 129 · see citing works
Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates
Franz, C., Askjaer, P., Antonin, W., et al. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600825 · Cited by 116 · see citing works
Self-citationDirect membrane protein–DNA interactions required early in nuclear envelope assembly
Ulbert, S., Platani, M., Boué, S., et al. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200512078 · Cited by 111 · see citing works
Nehrbass, U., Rout, M. P., Maguire, S., et al. (1996) · The Journal of Cell Biology · doi:10.1083/jcb.133.6.1153 · Cited by 91 · see citing works
Karyopherins in nuclear pore biogenesis
Lusk, C. P., Makhnevych, T., Marelli, M., et al. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200203079 · Cited by 83 · see citing works
Importin α associates with membranes and participates in nuclear envelope assembly in vitro
Hachet, V., Köcher, T., Wilm, M. S., et al. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600154 · Cited by 83 · see citing works
Nuclear Pore Protein gp210 Is Essential for Viability in HeLa Cells andCaenorhabditis elegans
Cohen, M., Feinstein, N., Wilson, K. L., et al. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-04-0260 · Cited by 60 · see citing works
Identification of a New Vertebrate Nucleoporin, Nup188, with the Use of a Novel Organelle Trap Assay
Miller, B., Powers, M. A., Park, M., et al. (2000) · Molecular Biology of the Cell · doi:10.1091/mbc.11.10.3381 · Cited by 57 · see citing works
Lau, C. K., Giddings, T. H., & Winey, M. (2004) · Eukaryotic Cell · doi:10.1128/ec.3.2.447-458.2004 · Cited by 57 · see citing works
The Integral Membrane Protein Pom34p Functionally Links Nucleoporin Subcomplexes
Miao, M., Ryan, K. J., & Wente, S. R. (2005) · Genetics · doi:10.1534/genetics.105.052068 · Cited by 53 · see citing works
Nuclear Import of the Stem–Loop Binding Protein and Localization during the Cell Cycle
Erkmann, J. A., Wagner, E. J., Dong, J., et al. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-11-1023 · Cited by 42 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
D'Angelo, M. A., Raices, M., Panowski, S. H., & Hetzer, M. W. (2009) · Cell · doi:10.1016/j.cell.2008.11.037 · Cited by 565 · see citing works
Probing nuclear pore complex architecture with proximity-dependent biotinylation
Kim, D. I., KC, B., Zhu, W., Motamedchaboki, K., Doye, V., & Roux, K. J. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1406459111 · Cited by 560 · see citing works
AI-based structure prediction empowers integrative structural analysis of human nuclear pores
Mosalaganti, S., Obarska-Kosinska, A., Siggel, M., Taniguchi, R., Turoňová, B., Zimmerli, C. E., Buczak, K., Schmidt, F. H., Margiotta, E., Mackmull, M.-T., et al. (2022) · Science · doi:10.1126/science.abm9506 · Cited by 355 · see citing works
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody
Daniel, K., Icha, J., Horenburg, C., Müller, D., Norden, C., & Mansfeld, J. (2018) · Nature Communications · doi:10.1038/s41467-018-05855-5 · Cited by 113 · see citing works
Self-citationDimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly
Vollmer, B., Schooley, A., Sachdev, R., Eisenhardt, N., Schneider, A. M., Sieverding, C., Madlung, J., Gerken, U., Macek, B., & Antonin, W. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.256 · Cited by 104 · see citing works
Self-citationKind, B., Koehler, K., Lorenz, M., & Huebner, A. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.09.080 · Cited by 48 · see citing works
The transmembrane nucleoporin NDC1 is required for targeting of ALADIN to nuclear pore complexes
Yamazumi, Y., Kamiya, A., Nishida, A., Nishihara, A., Iemura, S.-I., Natsume, T., & Akiyama, T. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.08.096 · Cited by 34 · see citing works
- Authors
- Mansfeld, J., Güttinger, S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., Kutay, U., & Antonin, W.
- Affiliations
- ETH Zurich (CH)
- University of Alberta (CA)
- University of British Columbia (CA)
- European Molecular Biology Laboratory (DE)
- Corresponding author
- Mattaj, Iain W.
- Kutay, Ulrike
- ORCID
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2006-04
- Volume
- 22
- Issue
- 1
- Pages
- 93-103
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2096814753
- Cited by
- 221
- References
- 35
- Citations by year
- 2025: 5 · 2024: 7 · 2023: 15 · 2022: 9 · 2021: 7 · 2020: 10 · 2019: 9 · 2018: 8 · 2017: 8 · 2016: 7 · 2015: 14 · 2014: 11 · 2013: 13 · 2012: 12
- FWCI
- 8.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- NDC1, nuclear pore complex assembly, nucleoporins, nuclear envelope, Nup93, NUP205, nuclear transport
- MeSH terms
- Amino Acid Sequence, Animals, Cell Membrane, Cloning, Molecular, HeLa Cells, Humans, Molecular Sequence Data, Nuclear Envelope, Peptide Fragments, Proteolipids, Rabbits, Osteosarcoma, Xenopus laevis, Conserved Sequence, Sequence Homology, Amino Acid, Nuclear Pore, Nuclear Pore Complex Proteins, Xenopus Proteins, RNA, Small Interfering
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review