Cited by 221Nuclear Structure and FunctionMicrotubule and mitosis dynamicsRNA Research and Splicing

The Conserved Transmembrane Nucleoporin NDC1 Is Required for Nuclear Pore Complex Assembly in Vertebrate Cells

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Mansfeld, Jörg, Güttinger, Stephan, Hawryluk-Gara, Lisa A., Panté, Nelly, Mall, Moritz, Galy, Vincent, Haselmann, Uta, Mühlhäusser, Petra +4 more·2006·Molecular Cell

Abstract

Nuclear pore complexes (NPCs) are large proteinaceous channels embedded in the nuclear envelope (NE), through which exchange of molecules between the nucleus and cytosol occurs. Biogenesis of NPCs is complex and poorly understood. In particular, almost nothing is known about how NPCs are anchored in the NE. Here, we characterize vertebrate NDC1--a transmembrane nucleoporin conserved between yeast and metazoans. We show by RNA interference (RNAi) and biochemical depletion that NDC1 plays an important role in NPC and NE assembly in vivo and in vitro. RNAi experiments suggest a functional link between NDC1 and the soluble nucleoporins Nup93, Nup53, and Nup205. Importantly, NDC1 interacts with Nup53 in vitro. This suggests that NDC1 function involves forming a link between the NE membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane.

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Crossref · 10.1016/j.molcel.2006.02.015

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Authors
Mansfeld, J., Güttinger, S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., Kutay, U., & Antonin, W.
Affiliations
  • ETH Zurich (CH)
  • University of Alberta (CA)
  • University of British Columbia (CA)
  • European Molecular Biology Laboratory (DE)
Corresponding author
  • Mattaj, Iain W.
  • Kutay, Ulrike
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2006-04
Volume
22
Issue
1
Pages
93-103
ISSN
1097-2765 (ISSN-L)
OpenAlex
W2096814753
Cited by
221
References
35
Citations by year
2025: 5 · 2024: 7 · 2023: 15 · 2022: 9 · 2021: 7 · 2020: 10 · 2019: 9 · 2018: 8 · 2017: 8 · 2016: 7 · 2015: 14 · 2014: 11 · 2013: 13 · 2012: 12
FWCI
8.11
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
NDC1, nuclear pore complex assembly, nucleoporins, nuclear envelope, Nup93, NUP205, nuclear transport
MeSH terms
Amino Acid Sequence, Animals, Cell Membrane, Cloning, Molecular, HeLa Cells, Humans, Molecular Sequence Data, Nuclear Envelope, Peptide Fragments, Proteolipids, Rabbits, Osteosarcoma, Xenopus laevis, Conserved Sequence, Sequence Homology, Amino Acid, Nuclear Pore, Nuclear Pore Complex Proteins, Xenopus Proteins, RNA, Small Interfering

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