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A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway
Open accessAbstract
The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.
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“For the remaining 260 genes, the stocks used the alternative integration site which is not problematic, with KK stocks having been used successfully in a range of different screens [29,34].”
From Functional unknomics: Systematic screening of conserved genes of unknown function (2023) · cites this paper as [34]
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M., & Salzberg, S. L. (2009) · Genome Biology · doi:10.1186/gb-2009-10-3-r25 · Cited by 21,849
Differential expression analysis for sequence count data
Anders, S., & Huber, W. (2010) · Genome Biology · doi:10.1186/gb-2010-11-10-r106 · Cited by 15,581
Trapnell, C., Williams, B. A., Pertea, G., Mortazavi, A., Kwan, G., van Baren, M. J., Salzberg, S. L., Wold, B. J., & Pachter, L. (2010) · Nature Biotechnology · doi:10.1038/nbt.1621 · Cited by 15,556
TopHat: discovering splice junctions with RNA-Seq
Trapnell, C., Pachter, L. S., & Salzberg, S. L. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp120 · Cited by 11,682
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Dietzl, G., Chen, D., Schnorrer, F., Su, K.-C., Barinova, Y., Fellner, M., Gasser, B., Kinsey, K., Oppel, S., Scheiblauer, S., et al. (2007) · Nature · doi:10.1038/nature05954 · Cited by 2,644
Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila
Brennecke, J., Aravin, A. A., Stark, A., Dus, M., Kellis, M., Sachidanandam, R., & Hannon, G. J. (2007) · Cell · doi:10.1016/j.cell.2007.01.043 · Cited by 2,524
Self-citationTransposable elements and the epigenetic regulation of the genome
Slotkin, R. K., & Martienssen, R. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2072 · Cited by 2,044
A germline-specific class of small RNAs binds mammalian Piwi proteins
Girard, A., Sachidanandam, R., Hannon, G. J., & Carmell, M. A. (2006) · Nature · doi:10.1038/nature04917 · Cited by 1,762
Self-citationA novel class of small RNAs bind to MILI protein in mouse testes
Aravin, A., Gaidatzis, D., Pfeffer, S., Lagos-Quintana, M., Landgraf, P., Iovino, N., Morris, P., Brownstein, M. J., Kuramochi-Miyagawa, S., Nakano, T., et al. (2006) · Nature · doi:10.1038/nature04916 · Cited by 1,516
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Items 201–270 of 270
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Shu, L., Ma, Y., Wang, Y., Chang, Y., & Du, Y.-Z. (2025) · Journal of Thermal Biology · doi:10.1016/j.jtherbio.2025.104341 · Cited by 2
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Hu, K., Sha, X., Zu, C., Yang, F., Wang, G., Cheng, H., Li, K., Gao, Y., Xu, C., Geng, H., et al. (2025) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2025.104974 · Cited by 2
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Monteiro, V., Yu, C. W., Roselli, C., Bakthavachalu, B., Ramaswami, M., & Hurd, T. R. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.13.659559 · Cited by 2
Maternal ovo represses the expression of transposable elements in adult ovaries
Hayashi, M., Koga, Y., Kozono, Y., & Kobayashi, S. (2025) · Developmental Biology · doi:10.1016/j.ydbio.2025.04.014 · Cited by 1
Noncoding RNAs in Genome Integrity
Kovalchuk, I. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-803309-8.00025-2 · Cited by 1
piRNA Biogenesis in Drosophila Ovary
XiaoNa, W. A. N. G., DaHua, C. H. E. N., & HaiLong, W. A. N. G. (2016) · Scientia Sinica Vitae · doi:10.1360/n052015-00224 · Cited by 1
Almeida, M. V. (2018) · Data Archiving and Networked Services (DANS) · doi:10.17632/xyxh6hv3mc.1 · Cited by 1
Kukushkina, I. V., Makhnovskii, P. A., Нефедова, Л. Н., Балакирева, Е. А., Romanova, N. I., Kuzmin, I. V., Lavrenov, A. R., & Kim, A. I. (2020) · Molecular Biology · doi:10.1134/s0026893320030097 · Cited by 1
Drosophila Genetic Resources for Elucidating piRNA Pathway
Saito, K. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2380-0_8 · Cited by 1
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Szabó, A., Borkúti, P., Kovács, Z., Kristó, I., Abonyi, C., & Vilmos, P. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-2970-3_16 · Cited by 1
Structural basis of thymidine-rich DNA recognition by Drosophila P75 PWWP domain
Jin, Z., Meng, Z., Liu, Y., Li, C., Zhang, X., Yin, Y., Gao, G., Dou, K., & Huang, Y. (2025) · Communications Biology · doi:10.1038/s42003-025-07895-2 · Cited by 1
Li, C., Meng, Z., Wen, Q., Yang, W., Xu, Y., Lyu, Y., Guo, Y., Lyu, T., Shen, D., & Dou, K. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf903 · Cited by 1
The Role of Noncoding RNAs in Genome Stability and Aging
Kovalchuk, I. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-811060-7.00008-5 · Cited by 1
Meseure, D., & Alsibai, K. D. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.79974 · Cited by 4
Zinshteyn, D., & Barbash, D. A. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.18.435951 · Cited by 1
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Signor, S. A., Vedanayagam, J., Kim, B. Y., Wierzbicki, F., Kofler, R., & Lai, E. C. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.09.29.510127 · Cited by 1
McQuarrie, D. W. J., Alizada, A., Czech, B., & Soller, M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.01.564449 · Cited by 1
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piRNA Biogenesis and Transposon Silencing in Drosophila: A Dissertation
Zhang, Z. (2013) · The Journal of the American Medical Association (JAMA) Network (American Medical Association) · doi:10.13028/m2888d
The Role of Mobile Elements in the Evolution and Function of HSPS Systems
Evgen’ev, M. B., Garbuz, D. G., & Zatsepina, O. G. (2014) · Springer eBooks · doi:10.1007/978-94-017-9235-6_6
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Strein, J. (2015) · Zenodo (CERN European Organization for Nuclear Research) · doi:10.5281/zenodo.48371
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Russell, S. J. (2016) · The Atrium (University of Guelph)
Ma, X. (2014) · KU ScholarWorks (The University of Kansas)
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Haac, M. E. (2013) · VTechWorks (Virginia Tech)
Erwin, A. A., & Blumenstiel, J. P. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/342105
Jankovics, F., Bence, M., Sinka, R., Faragó, A., Bodai, L., Pettkó‐Szandtner, A., Ibrahim, K., Takács, Z., Szarka-Kovács, A. B., & Erdélyi, M. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/383265
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Homolka, D., & Pillai, R. S. (2019) · Nature Structural & Molecular Biology · doi:10.1038/s41594-019-0294-y
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Dou, K., Liu, Y., Zhang, Y., Wang, C., Huang, Y., & Zhang, Z. Z. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/856021
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Andreev, V. I., Yu, C., Wang, J., Schnabl, J., Tirián, L., Gehre, M., Handler, D., Duchek, P., Novatchkova, M., Baumgartner, L., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.07.27.453956
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Szabó, A. (2021) · SZTE Repository of Dissertations (University of Szeged) · doi:10.14232/phd.10786
Citovsky, V., & Keren, I. (2020) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.737030657.793572884
Faculty Opinions recommendation of p53 genes function to restrain mobile elements.
Attardi, L. D. (2017) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.726037451.793537675
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Lawlor, M. A., Cao, W., & Ellison, C. E. (2023) · doi:10.31219/osf.io/btvmh
Chavan, A., Skrutl, L., Uliana, F., Pfister, M., Brändle, F., Tirián, L., Baptista, D., Handler, D., Burke, D. F., Sintsova, A., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.07.11.548599
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Kukushkina, I. V., Lavrenov, A. R., Milyaeva, P. A., Lavrenova, A. I., Kuzmin, I. V., Нефедова, Л. Н., & Kim, A. I. (2024) · Molecular Biology · doi:10.1134/s0026893324700419
Kawaguchi, S., Xin, X., Takashi, S., Kenta, Y., Ryuuya, K., Ryota, S., Susumu, D., & Kai, T. (2024) · eLife · doi:10.7554/elife.101967.1
Kawaguchi, S., Xin, X., Takashi, S., Kenta, Y., Ryuuya, K., Ryota, S., Susumu, D., & Kai, T. (2025) · eLife · doi:10.7554/elife.101967.2
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Saito, R., Ishizu, H., Harigai, R., Murano, K., Namba, Y., & Siomi, M. C. (2025) · eLife · doi:10.7554/elife.107358.1
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Liu, D., Guo, Y., Shen, M., Lv, J. G., Wei, P., Jia, L., & Ma, S. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.10.09.681363
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Saito, R., Ishizu, H., Harigai, R., Murano, K., Namba, Y., & Siomi, M. C. (2025) · eLife · doi:10.7554/elife.107358.2
McQuarrie, D. W. J., Alizada, A., Czech, B., & Soller, M. (2024) · Figshare · doi:10.6084/m9.figshare.26211422
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Nallasivan, M. P., Haussmann, I. U., Civetta, A., & Soller, M. (2021) · Figshare · doi:10.6084/m9.figshare.16834910
McQuarrie, D. W. J., Alizada, A., Czech, B., & Soller, M. (2024) · Figshare · doi:10.6084/m9.figshare.26211636
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Hüttinger, L., Kober, L., Zimmermann, M., Schlensog, S., Engelhart, M., & Nagel, A. C. (2026) · Cellular and Molecular Life Sciences · doi:10.64898/2026.03.11.711025 · Cited by 1
Bellec, K., Carroll, L. R., Pennel, K. A. F., Tian, Y., Yu, Y., Akan, A. B., Billard, C. V., Doleschall, N. J., Cameron, A. R., Herédia, F., et al. (2026) · Cell Reports · doi:10.1016/j.celrep.2026.117186 · Cited by 1
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Matias, N. R. (2015) · theses.fr (ABES) · doi:10.70675/888fa02az6711z46d7zb36bza653735d27ef
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Karlikow, M. (2015) · theses.fr (ABES) · doi:10.70675/71a4585bz0f5bz47a2zb439z7d0cf66a0cb6
Théron, E. (2017) · theses.fr (ABES) · doi:10.70675/d76b18c1zc2c1z4748z9fc2zcc393afc2a86
Regulation of wing growth in vivo by the histone demethylase dLSD1
Texier, M. (2018) · theses.fr (ABES) · doi:10.70675/39466764za985z4be7z8494z3c0281e77b30
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Griffin, J. S., Harney, E., Capes, C., Connell, R., Betancourt, A. J., & Romero‐Soriano, V. (2026) · Genetics · doi:10.1093/genetics/iyag219
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Chen, P., Xu, P., Waseem, H., McCormick, J., Pan, K. C., Aravin, A. A., Clark, A. G., & Feschotte, C. (2026) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2623866123
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Metadata
- Authors
- Czech, B., Preall, J. B., McGinn, J., & Hannon, G. J.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Czech, Benjamin
- Hannon, Gregory J.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 50
- Issue
- 5
- Pages
- 749-761
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC3724427
- OpenAlex
- W1997570561
- Cited by
- 269
- References
- 62
- Citations by year
- 2026: 8 · 2025: 24 · 2024: 16 · 2023: 20 · 2022: 11 · 2021: 26 · 2020: 23 · 2019: 21 · 2018: 24 · 2017: 21 · 2016: 16 · 2015: 25 · 2014: 26 · 2013: 8
- FWCI
- 30.19
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- American Cancer Society
- Boehringer Ingelheim Fonds
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM062534, R37GM062534)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- piRNA pathway, germline, transposon silencing, RNAi screen, Drosophila ovary, Piwi, eggplant, deadlock, selfish genetic elements
- MeSH terms
- Animals, DNA Transposable Elements, Drosophila melanogaster, Female, Microtubule-Associated Proteins, Ovary, Peptide Initiation Factors, Reproducibility of Results, Gene Silencing, Gene Expression Profiling, Drosophila Proteins, RNA Interference, RNA, Small Interfering, Argonaute Proteins
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