Cited by 270Chromosomal and Genetic VariationsRNA Interference and Gene DeliveryEpigenetics and DNA Methylation

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A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway

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Czech, Benjamin, Preall, Jonathan B., McGinn, Jon, Hannon, Gregory J.·2013·Molecular Cell

DOI 10.1016/j.molcel.2013.04.007PMC PMC3724427

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Abstract

The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.

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Self-citation

Authors: Czech, B., Preall, J. B., McGinn, J., & Hannon, G. J.
Affiliations: Howard Hughes Medical Institute (US)
Corresponding author: Czech, Benjamin
Hannon, Gregory J.
ORCID: Preall, Jonathan B.
McGinn, Jon
Hannon, Gregory J.
Venue: Molecular Cell
Publisher: Elsevier BV
Published: 2013-06
Volume: 50
Issue: 5
Pages: 749-761
ISSN: 1097-2765 (ISSN-L)
DOI: 10.1016/j.molcel.2013.04.007
PMC: PMC3724427
OpenAlex: W1997570561
Cited by: 269
References: 62
Citations by year: 2026: 8 · 2025: 24 · 2024: 16 · 2023: 20 · 2022: 11 · 2021: 26 · 2020: 23 · 2019: 21 · 2018: 24 · 2017: 21 · 2016: 16 · 2015: 25 · 2014: 26 · 2013: 8
FWCI: 30.19
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 370 d)
Locations: Molecular Cell (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
Funders: Howard Hughes Medical Institute
American Cancer Society
Boehringer Ingelheim Fonds
National Institutes of Health
National Institute of General Medical Sciences (R01GM062534, R37GM062534)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords: piRNA pathway, germline, transposon silencing, RNAi screen, Drosophila ovary, Piwi, eggplant, deadlock, selfish genetic elements
MeSH terms: Animals, DNA Transposable Elements, Drosophila melanogaster, Female, Microtubule-Associated Proteins, Ovary, Peptide Initiation Factors, Reproducibility of Results, Gene Silencing, Gene Expression Profiling, Drosophila Proteins, RNA Interference, RNA, Small Interfering, Argonaute Proteins

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A Transcriptome-wide RNAi Screen in the Drosophila Ovary Reveals Factors of the Germline piRNA Pathway

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