The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
eIF5A Functions Globally in Translation Elongation and Termination
Open accessAbstract
SUMMARY The eukaryotic translation factor eIF5A, originally identified as an initiation factor, was later shown to promote translation elongation of iterated proline sequences. Using a combination of ribosome profiling and in vitro biochemistry, we report a much broader role for eIF5A in elongation and uncover a critical function for eIF5A in termination. Ribosome profiling of an eIF5A-depleted strain reveals a global elongation defect, with abundant ribosomes stalling at many sequences, not limited to proline stretches. Our data also show ribosome accumulation at stop codons and in the 3′ UTR, suggesting a global defect in termination in the absence of eIF5A. Using an in vitro reconstituted translation system, we find that eIF5A strongly promotes the translation of the stalling sequences identified by profiling and increases the rate of peptidyl-tRNA hydrolysis more than 17-fold. We conclude that eIF5A functions broadly in elongation and termination, rationalizing its high cellular abundance and essential nature.
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Cites
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eIF5A Promotes Translation of Polyproline Motifs
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Self-citationThe Elongation, Termination, and Recycling Phases of Translation in Eukaryotes
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Self-citationEF-P Is Essential for Rapid Synthesis of Proteins Containing Consecutive Proline Residues
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Translation Elongation Factor EF-P Alleviates Ribosome Stalling at Polyproline Stretches
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Polyamines control eIF5A hypusination, TFEB translation, and autophagy to reverse B cell senescence
Zhang, H., Alsaleh, G., Feltham, J., Sun, Y., Napolitano, G., Riffelmacher, T., Charles, P., Frau, L., Hublitz, P., Yu, Z., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.08.005 · Cited by 341
Wu, C. C.-C., Zinshteyn, B., Wehner, K. A., & Green, R. (2019) · Molecular Cell · doi:10.1016/j.molcel.2018.12.009 · Cited by 294
Self-citationMachado, L., Geara, P., Camps, J., Dos Santos, M., Teixeira-Clerc, F., Van Herck, J., Varet, H., Legendre, R., Pawlotsky, J.-M., Sampaolesi, M., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.01.017 · Cited by 170
Liang, Y., Piao, C., Beuschel, C. B., Toppe, D., Kollipara, L., Bogdanow, B., Maglione, M., Lützkendorf, J., See, J. C. K., Huang, S., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.108941 · Cited by 130
Tan, T. C. J., Kelly, V., Zou, X., Wright, D., Ly, T., & Zamoyska, R. (2022) · Nature Communications · doi:10.1038/s41467-022-35252-y · Cited by 30
Metadata
- Authors
- Schuller, A. P., Wu, C. C.-C., Dever, T. E., Buskirk, A. R., & Green, R.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2017-04
- Volume
- 66
- Issue
- 2
- Pages
- 194-205.e5
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2604467178
- Cited by
- 477
- References
- 54
- FWCI
- 13.69
- Type
- journal article
- Language
- en
- Open access
- bronze
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