The DOI matches its Crossref record; 137 of the 139 references it cites were found in a registry or an index.
Journey to the Center of the Cell: Tracing the Path of AAV Transduction
Open accessAbstract
As adeno-associated virus (AAV)-based gene therapies are being increasingly approved for use in humans, it is important that we understand vector-host interactions in detail. With the advances in genome-wide genetic screening tools, a clear picture of AAV-host interactions is beginning to emerge. Understanding these interactions can provide insights into the viral life cycle. Accordingly, novel strategies to circumvent the current limitations of AAV-based vectors may be explored. Here, we summarize our current understanding of the various stages in the journey of the vector from the cell surface to the nucleus and contextualize the roles of recently identified host factors.
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“After endosomal escape, rAAV particles either enter the nucleus for transgene expression, or become ubiquitylated then degraded by proteasome (84).”
From Immunomodulation in Administration of RAAV: Preclinical and Clinical Adjuvant Pharmacotherapies (2021) · cites this paper as [84]
In the citing paper
“However, endosomal acidification is essential for rAAV escape (84), and hydroxychloroquine increases endosomal and lysosomal pH (60), this effect may not be replicated or consistent with systemic application.”
From Immunomodulation in Administration of RAAV: Preclinical and Clinical Adjuvant Pharmacotherapies (2021) · cites this paper as [84]
Citations
Cites
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Adeno-Associated Virus (AAV) as a Vector for Gene Therapy
Naso, M. F., Tomkowicz, B., Perry, W. L., & Strohl, W. R. (2017) · BioDrugs · doi:10.1007/s40259-017-0234-5 · Cited by 1,362
Engineering adeno-associated virus vectors for gene therapy
Li, C., & Samulski, R. J. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0205-4 · Cited by 1,175
Adenovirus-Associated Defective Virus Particles
Atchison, R. W., Casto, B. C., & Hammon, W. M. (1965) · Science · doi:10.1126/science.149.3685.754 · Cited by 1,004
Emerging issues in AAV-mediated in vivo gene therapy
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Real-Time Single-Molecule Imaging of the Infection Pathway of an Adeno-Associated Virus
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Cited by
Sorted by citations
Adeno-associated virus as a delivery vector for gene therapy of human diseases
Wang, J.-H., Gessler, D. J., Zhan, W., Gallagher, T. L., & Gao, G. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01780-w · Cited by 904
“40 For example, rAAV2 binds to heparan sulphate proteoglycan (HSPG), rAAV1, rAAV4, and rAAV5 primarily interact with sialic acid, and rAAV9 interacts with N-linked galactose.”
BackgroundAdeno-associated virus (AAV) cell entry: structural insights
Meyer, N., & Chapman, M. S. (2021) · Trends in Microbiology · doi:10.1016/j.tim.2021.09.005 · Cited by 144
Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase
Witte, I. P., Lampe, G. D., Eitzinger, S., Miller, S. M., Berríos, K. N., McElroy, A. N., King, R. T., Stringham, O. G., Gelsinger, D. R., Vo, P. L. H., et al. (2025) · Science · doi:10.1126/science.adt5199 · Cited by 121
Li, X., Salvia, S. L., Liang, Y., Adamiak, M., Kohlbrenner, E., Jeong, D., Chepurko, E., Ceholski, D. K., López-Gordo, E., Yoon, S., et al. (2023) · Circulation · doi:10.1161/circulationaha.122.063759 · Cited by 92
Understanding AAV vector immunogenicity: from particle to patient
Dhungel, B. P., Winburn, I. C., Pereira, C. D. F., Huang, K., Chhabra, A., & Rasko, J. E. J. (2024) · Theranostics · doi:10.7150/thno.89380 · Cited by 97
“…most serotypes can be found in the majority of subjects [14, 31] AAV, adeno-associated virus; NAb, neutralizing antibody; rAAV, recombinant adeno-associated virus AAVs consist of a single-stranded DNA (ssDNA) genome of ~4.7 Kb enclosed by a capsid ~26 nm in diameter ( Figure 1A ) [10, 11].”
BackgroundInfluentialSelf-citation
Metadata
- Authors
- Dhungel, B. P., Bailey, C. G., & Rasko, J. E. J.
- Affiliations
- The University of Sydney (AU)
- Corresponding author
- Rasko, John E.J.
- Venue
- Trends in Molecular Medicine
- Publisher
- Elsevier BV
- Published
- 2021-02
- Volume
- 27
- Issue
- 2
- Pages
- 172-184
- ISSN
- 1471-4914 (ISSN-L)
- OpenAlex
- W3092811898
- Cited by
- 122
- References
- 139
- Citations by year
- 2026: 32 · 2025: 29 · 2024: 21 · 2023: 17 · 2022: 9 · 2021: 14
- FWCI
- 1.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Trends in Molecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NSW Health
- National Health and Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- adeno-associated virus, adeno-associated virus vectors, gene therapy, vector-host interactions, viral life cycle, host factors, nuclear translocation, viral transduction, genome-wide screen
- MeSH terms
- Host Microbial Interactions, Dependovirus, Animals, Biological Transport, Genetic Vectors, Humans, Transduction, Genetic, Genetic Therapy, Gene Transfer Techniques, Cell Tracking
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