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Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

Open access
Li, Jingying, Sun, Yongwei, Du, Jinlu, Zhao, Yunde, Xia, Lanqin·2017·Molecular Plant

Abstract

CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated Cas9 endonuclease)-mediated genome editing has revolutionized biological research and crop improvement because of its specificity, simplicity, and versatility (reviewed in Komor et al., 2016a). Editing a gene by CRISPR/Cas9 only has three requirements: (1) expression of the nuclear localized Cas9 protein; (2) production of a guide RNA (gRNA) molecule, whose first 20 nucleotides are complementary to the target gene; (3) the NGG PAM site that is located immediately adjacent to the 3′ end of the target sequence (Figure 1A).

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Metadata

Authors
Li, J., Sun, Y., Du, J., Zhao, Y., & Xia, L.
Venue
Molecular Plant
Publisher
Elsevier BV
Published
2017-03
Volume
10
Issue
3
Pages
526-529
ISSN
1674-2052 (ISSN-L)
OpenAlex
W2560407750
Cited by
302
References
1
FWCI
17.15
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System. https://app.cochat.ai/papers/10.1016/j.molp.2016.12.001
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Li, J., Sun, Y., Du, J., Zhao, Y., & Xia, L. (2017). Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System. Molecular Plant. https://doi.org/10.1016/j.molp.2016.12.001

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