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Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
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Later base editors' generations built upon this system to increase efficiency and reduce off-target indel formation.
“Later base editors’ generations built upon this system to increase efficiency and reduce off-target indel formation [83,84,85].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [84]
The paper’s introductory passage (indexed under Abstract; no page given) says the third-generation editor BE3 achieves higher efficiency and lower indel frequency than other genome-editing methods. Its account of the newer variants instead emphasizes expanded targeting and improved selectivity, including reduced off-target base editing—not reduced off-target indel formation. It also reports decreased editing efficiency for some variants. (LLM, conf=0.87)
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“In addition, base editors that use natural or engineered Cas variants with alternative PAM preferences have been reported (Kim et al., 2017; Kleinstiver et al., 2019; Miller et al., 2020; Nishimasu et al., 2018; Walton et al., 2020), increasing the density of editable target sites, although how…”
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Metadata
- Authors
- Kim, Y. B., Komor, A. C., Levy, J. M., Packer, M. S., Zhao, K. T., & Liu, D. R.
- ORCID
- Levy, Jonathan M
- Venue
- Nature Biotechnology
- Published
- 2017-02-13
- Volume
- 35
- Issue
- 4
- Pages
- 371-376
- ISSN
- 1087-0156
- 1546-1696
- PMC
- PMC5388574
- Cited by
- 758
- References
- 7
- Language
- en
- Licence
- www.springer.com/tdm (tdm)
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