Cited by 812CRISPR and Genetic EngineeringRNA regulation and diseasePlant Genetic and Mutation Studies

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Shimatani, Zenpei, Kashojiya, Sachiko, Takayama, Mariko, Terada, Rie, Arazoe, Takayuki, Ishii, Hisaki, Teramura, Hiroshi, Yamamoto, Tsuyoshi +6 more·2017·Nature Biotechnology

Abstract

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

Integrity signals

Registry

Crossref · 10.1038/nbt.3833

Cites

17 of 17 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T., & Kondo, A.
Affiliations
  • Kobe University (JP)
  • University of Tsukuba (JP)
  • Meijo University (JP)
Corresponding author
  • Ezura, Hiroshi
  • Nishida, Keiji
  • Ariizumi, Tohru
Venue
Nature Biotechnology
Published
2017-03-27
Volume
35
Issue
5
Pages
441-443
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
OpenAlex
W2598674316
Cited by
812
References
17
Citations by year
2026: 43 · 2025: 71 · 2024: 82 · 2023: 83 · 2022: 117 · 2021: 113 · 2020: 107 · 2019: 97 · 2018: 62 · 2017: 37
FWCI
33.83
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science (15K18647, 16K14654, 16K07560)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
Keywords
base editing, CRISPR/Cas9, rice, tomato, point mutagenesis, herbicide resistance, single guide RNA, crop improvement
MeSH terms
Gene Editing, Cytidine Deaminase, Recombinant Fusion Proteins, Oryza, Mutagenesis, Site-Directed, Genes, Plant, Point Mutation, Solanum lycopersicum, DNA, Plant, Base Pairing, Plants, Genetically Modified, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Associated Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review