Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Abstract
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Herbicide-resistant barley lines can be developed through base editing.
“More importantly, herbicide-resistant barley lines can be developed through base editing (Shimatani et al., 2017;Tian et al., 2018).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Shimatani et al., 2017
Cites
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437 · see citing works
CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool
Stemmer, M., Thumberger, T., del Sol Keyer, M., Wittbrodt, J., & Mateo, J. L. (2015) · PLoS ONE · doi:10.1371/journal.pone.0124633 · Cited by 1,088 · see citing works
CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants
Yang, L., Lü, L., Liu, H., Li, S., Feng, X., & Chen, L. (2014) · Molecular Plant · doi:10.1093/mp/ssu044 · Cited by 796 · see citing works
Fauser, F., Schiml, S., & Puchta, H. (2014) · The Plant Journal · doi:10.1111/tpj.12554 · Cited by 704 · see citing works
Resistance to AHAS inhibitor herbicides: current understanding
Yu, Q. P., & Powles, S. B. (2013) · Pest Management Science · doi:10.1002/ps.3710 · Cited by 503 · see citing works
Ryan, M. D., King, A. M. Q., & Thomas, G. P. (1991) · Journal of General Virology · doi:10.1099/0022-1317-72-11-2727 · Cited by 496 · see citing works
Sun, H., Uchii, S., Watanabe, S., & Ezura, H. (2005) · Plant and Cell Physiology · doi:10.1093/pcp/pci251 · Cited by 476 · see citing works
Self-citationPrecise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System
Lu, Y., & Zhu, J. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.11.013 · Cited by 423 · see citing works
Rapid and efficientAgrobacterium-mediated transformation in rice
Toki, S. (1997) · Plant Molecular Biology Reporter · doi:10.1007/bf02772109 · Cited by 392 · see citing works
Efficient gene targeting by homologous recombination in rice
Terada, R., Urawa, H., Inagaki, Y., Tsugane, K., & Iida, S. (2002) · Nature Biotechnology · doi:10.1038/nbt737 · Cited by 327 · see citing works
Self-citationGeneration of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System
Li, J., Sun, Y., Du, J., Zhao, Y., & Xia, L. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.12.001 · Cited by 304 · see citing works
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
Mikami, M., Toki, S., & Endo, M. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0342-x · Cited by 290 · see citing works
Negative reciprocal interactions between gibberellin and cytokinin in tomato
Fleishon, S., Shani, E., Ori, N., & Weiss, D. W. (2011) · New Phytologist · doi:10.1111/j.1469-8137.2010.03616.x · Cited by 106 · see citing works
Genes that influence yield in tomato
Ariizumi, T., Shinozaki, Y., & Ezura, H. (2013) · Ikushugaku zasshi · doi:10.1270/jsbbs.63.3 · Cited by 103 · see citing works
Self-citationTerada, R., Asao, H., & Iida, S. (2004) · Plant Cell Reports · doi:10.1007/s00299-003-0752-0 · Cited by 64 · see citing works
Self-citationDang, T. T., Shimatani, Z., Kawano, Y., Terada, R., & Shimamoto, K. (2013) · Plant and Cell Physiology · doi:10.1093/pcp/pct147 · Cited by 25 · see citing works
Self-citationCharacterization of autonomous Dart1 transposons belonging to the hAT superfamily in rice
Shimatani, Z., Takagi, K., Eun, C., Maekawa, M., Takahara, H., Hoshino, A., Qian, Q., Terada, R., Johzuka‐Hisatomi, Y., Iida, S., et al. (2009) · Molecular Genetics and Genomics · doi:10.1007/s00438-008-0410-x · Cited by 15 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237 · see citing works
Impact of Climate Change on Crops Adaptation and Strategies to Tackle Its Outcome: A Review
Raza, A., Razzaq, A., Mehmood, S. S., Zou, X., Zhang, X., Lv, Y., & Xu, J. (2019) · Plants · doi:10.3390/plants8020034 · Cited by 1,762 · see citing works
“Without any transformation of a gene, wheat has been developed with a low gluten level by using CRISPR/Cas9 technique [246].”
BackgroundInfluentialBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675 · see citing works
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture
Chen, K., Wang, Y., Zhang, R., Zhang, H., & Gao, C. (2019) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050718-100049 · Cited by 1,643 · see citing works
Genome engineering for crop improvement and future agriculture
Gao, C. (2021) · Cell · doi:10.1016/j.cell.2021.01.005 · Cited by 1,037 · see citing works
Applications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870 · see citing works
Komor, A. C., Zhao, K. T., Packer, M. S., Gaudelli, N. M., Waterbury, A. L., Koblan, L. W., Kim, Y. B., Badran, A. H., & Liu, D. R. (2017) · Science Advances · doi:10.1126/sciadv.aao4774 · Cited by 766 · see citing works
CRISPR for Crop Improvement: An Update Review
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Shilpha, J., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 698 · see citing works
A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Čermák, T., Curtin, S. J., Gil‐Humanes, J., Čegan, R., Kono, T. J. Y., Konečná, E., Belanto, J. J., Starker, C. G., Mathre, J. W., Greenstein, R. L., et al. (2017) · The Plant Cell · doi:10.1105/tpc.16.00922 · Cited by 648 · see citing works
EditR: A Method to Quantify Base Editing from Sanger Sequencing
Kluesner, M. G., Nedveck, D. A., Lahr, W. S., Garbe, J. R., Abrahante, J. E., Webber, B. R., & Moriarity, B. S. (2018) · The CRISPR Journal · doi:10.1089/crispr.2018.0014 · Cited by 565 · see citing works
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Li, C., Zong, Y., Wang, Y., Jin, S., Zhang, D., Song, Q., Zhang, R., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1443-z · Cited by 499 · see citing works
Progress and prospects in plant genome editing
Yin, K., Gao, C., & Qiu, J. (2017) · Nature Plants · doi:10.1038/nplants.2017.107 · Cited by 436 · see citing works
An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities
Gehrke, J. M., Cervantes, O., Clement, K., Wu, Y., Zeng, J., Bauer, D. E., Pinello, L., & Joung, J. K. (2018) · Nature Biotechnology · doi:10.1038/nbt.4199 · Cited by 433 · see citing works
The emerging and uncultivated potential of CRISPR technology in plant science
Zhang, Y., Malzahn, A. A., Sretenovic, S., & Qi, Y. (2019) · Nature Plants · doi:10.1038/s41477-019-0461-5 · Cited by 404 · see citing works
Precise plant genome editing using base editors and prime editors
Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021) · Nature Plants · doi:10.1038/s41477-021-00991-1 · Cited by 387 · see citing works
Veillet, F., Perrot, L., Chauvin, L., Kermarrec, M.-P., Guyon‐Debast, A., Chauvin, J.-É., Nogué, F., & Mazier, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020402 · Cited by 370 · see citing works
Billon, P., Bryant, E. E., Joseph, S. A., Nambiar, T. S., Hayward, S. B., Rothstein, R., & Ciccia, A. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.08.008 · Cited by 363 · see citing works
Gene editing in plants: progress and challenges
Mao, Y., Botella, J. R., Liu, Y., & Zhu, J. (2019) · National Science Review · doi:10.1093/nsr/nwz005 · Cited by 343 · see citing works
Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing
Zhang, R., Liu, J., Chai, Z., Chen, S., Bai, Y., Zong, Y., Chen, K., Li, J., Jiang, L., & Gao, C. (2019) · Nature Plants · doi:10.1038/s41477-019-0405-0 · Cited by 340 · see citing works
ICE-CBF-COR Signaling Cascade and Its Regulation in Plants Responding to Cold Stress
Hwarari, D., Guan, Y., Ahmad, B., Movahedi, A., Min, T., Hao, Z., Lu, Y., Chen, J., & Yang, L. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23031549 · Cited by 339 · see citing works
CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications
Molla, K. A., & Yang, Y. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.03.008 · Cited by 336 · see citing works
Lin, C., Hsu, C., Yang, L., Lee, L., Fu, J., Cheng, Q., Wu, F., Hsiao, H. C. W., Zhang, Y., Zhang, R., et al. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12870 · Cited by 331 · see citing works
Engineering herbicide-resistant watermelon variety through CRISPR/Cas9-mediated base-editing
Tian, S., Jiang, L., Cui, X., Zhang, J., Guo, S., Li, M., Zhang, H., Ren, Y., Gong, G., Zong, M., et al. (2018) · Plant Cell Reports · doi:10.1007/s00299-018-2299-0 · Cited by 307 · see citing works
Precision genome engineering through adenine base editing in plants
Kang, B., Yun, J., Kim, S., Shin, Y., Ryu, J., Choi, M., Woo, J. W., & Kim, J. (2018) · Nature Plants · doi:10.1038/s41477-018-0178-x · Cited by 306 · see citing works
Nidhi, S., Anand, U., Olekšák, P., Tripathi, P., Lal, J. A., Thomas, G., Kuča, K., & Tripathi, V. S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073327 · Cited by 294 · see citing works
CRISPR Crops: Plant Genome Editing Toward Disease Resistance
Langner, T., Kamoun, S., & Belhaj, K. (2018) · Annual Review of Phytopathology · doi:10.1146/annurev-phyto-080417-050158 · Cited by 290 · see citing works
The delivery challenge: fulfilling the promise of therapeutic genome editing
van Haasteren, J., Li, J., Scheideler, O. J., Murthy, N., & Schaffer, D. V. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0565-5 · Cited by 285 · see citing works
Plant genome editing with TALEN and CRISPR
Malzahn, A. A., Lowder, L. G., & Qi, Y. (2017) · Cell & Bioscience · doi:10.1186/s13578-017-0148-4 · Cited by 280 · see citing works
Genome Edited Crops Touch the Market: A View on the Global Development and Regulatory Environment
Menz, J., Modrzejewski, D., Hartung, F., Wilhelm, R., & Sprink, T. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.586027 · Cited by 275 · see citing works
MicroTom Metabolic Network: Rewiring Tomato Metabolic Regulatory Network throughout the Growth Cycle
Li, Y., Chen, Y., Zhou, L., You, S., Deng, H., Chen, Y., Alseekh, S., Yuan, Y., Fu, R., Zhang, Z., et al. (2020) · Molecular Plant · doi:10.1016/j.molp.2020.06.005 · Cited by 270 · see citing works
Targeted genome-modification tools and their advanced applications in crop breeding
Li, B., Sun, C., Li, J., & Gao, C. (2024) · Nature Reviews Genetics · doi:10.1038/s41576-024-00720-2 · Cited by 259 · see citing works
Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture
Ricroch, A., Clairand, P., & Harwood, W. (2017) · Emerging Topics in Life Sciences · doi:10.1042/etls20170085 · Cited by 252 · see citing works
Rewritable multi-event analog recording in bacterial and mammalian cells
Tang, W., & Liu, D. R. (2018) · Science · doi:10.1126/science.aap8992 · Cited by 252 · see citing works
Genome editing with the CRISPR‐Cas system: an art, ethics and global regulatory perspective
Zhang, D., Hussain, A., Manghwar, H., Xie, K., Xie, S., Zhao, S., Larkin, R. M., Qing, P., Jin, S., & Ding, F. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13383 · Cited by 245 · see citing works
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
Kumar, M., Prusty, M. R., Pandey, M. K., Singh, P. K., Bohra, A., Guo, B. Z., & Varshney, R. K. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1157678 · Cited by 245 · see citing works
Inorganic arsenic toxicity and alleviation strategies in rice
Mawia, A. M., Hui, S., Zhou, L., Li, H., Tabassum, J., Lai, C., Wang, J., Shao, G., Wei, X., Tang, S., et al. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.124751 · Cited by 237 · see citing works
PAM-less plant genome editing using a CRISPR–SpRY toolbox
Ren, Q., Sretenovic, S., Liu, S., Tang, X. D., Huang, L., He, Y., Liu, L., Guo, Y., Zhong, Z., Liu, G., et al. (2021) · Nature Plants · doi:10.1038/s41477-020-00827-4 · Cited by 237 · see citing works
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes
Hess, G. T., Tycko, J., Yao, D., & Bassik, M. C. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.029 · Cited by 234 · see citing works
Base editing in crops: current advances, limitations and future implications
Mishra, R., Joshi, R. K., & Zhao, K. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13225 · Cited by 231 · see citing works
Ren, B., Yan, F., Kuang, Y., Li, N., Zhang, D., Zhou, X., Lin, H., & Zhou, H. (2018) · Molecular Plant · doi:10.1016/j.molp.2018.01.005 · Cited by 230 · see citing works
CRISPR base editors: genome editing without double-stranded breaks
Eid, A., Alshareef, S. A., & Mahfouz, M. M. (2018) · Biochemical Journal · doi:10.1042/bcj20170793 · Cited by 229 · see citing works
CRISPR technology is revolutionizing the improvement of tomato and other fruit crops
Wang, T., Zhang, H., & Zhu, H. (2019) · Horticulture Research · doi:10.1038/s41438-019-0159-x · Cited by 228 · see citing works
Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9
El‐Mounadi, K., Morales-Floriano, M. L., & García-Ruíz, H. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00056 · Cited by 227 · see citing works
Zaidi, S. S.-E.-A., Mahas, A., Vanderschuren, H., & Mahfouz, M. M. (2020) · Genome Biology · doi:10.1186/s13059-020-02204-y · Cited by 221 · see citing works
Deaminase-mediated multiplex genome editing in Escherichia coli
Banno, S., Nishida, K., Arazoe, T., Mitsunobu, H., & Kondo, A. (2018) · Nature Microbiology · doi:10.1038/s41564-017-0102-6 · Cited by 212 · see citing works
Self-citationZhao, H., Li, Y., He, L., Pu, W., Yu, W., Li, Y., Wu, Y., Xu, C., Wei, Y., Ding, Q., et al. (2019) · Circulation · doi:10.1161/circulationaha.119.042476 · Cited by 212 · see citing works
Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox
Razzaq, A., Saleem, F., Kanwal, M., Mustafa, G., Yousaf, S., Arshad, H. M. I., Hameed, M. K., Khan, M. S., & Joyia, F. A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20164045 · Cited by 210 · see citing works
Development of Plant Prime-Editing Systems for Precise Genome Editing
Xu, R., Li, J., Liu, X., Shan, T., Qin, R., & Wei, P. (2020) · Plant Communications · doi:10.1016/j.xplc.2020.100043 · Cited by 209 · see citing works
Genome Editing in Rice: Recent Advances, Challenges, and Future Implications
Mishra, R., Joshi, R. K., & Zhao, K. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.01361 · Cited by 204 · see citing works
CRISPR / dC as9 platforms in plants: strategies and applications beyond genome editing
Moradpour, M., & Abdulah, S. N. A. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13232 · Cited by 203 · see citing works
Madzak, C. (2021) · Journal of Fungi · doi:10.3390/jof7070548 · Cited by 203 · see citing works
Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG
Zhong, Z., Sretenovic, S., Ren, Q., Yang, L., Bao, Y., Qi, C., Yuan, M., He, Y., Liu, S., Liu, X., et al. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.011 · Cited by 198 · see citing works
Genome Editing in Plants: Exploration of Technological Advancements and Challenges
Vats, S., Kumawat, S., Kumar, V., Patil, G., Joshi, T., Sonah, H., Sharma, T. R., & Deshmukh, R. K. (2019) · Cells · doi:10.3390/cells8111386 · Cited by 191 · see citing works
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
Zhang, Y., Qin, W., Lu, X., Xu, J., Huang, H., Bai, H., Li, S., & Lin, S. (2017) · Nature Communications · doi:10.1038/s41467-017-00175-6 · Cited by 188 · see citing works
Trait discovery and editing in tomato
Rothan, C., Diouf, I., & Causse, M. (2018) · The Plant Journal · doi:10.1111/tpj.14152 · Cited by 188 · see citing works
Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM
Endo, M., Mikami, M., Endo, A., Kaya, H., Itoh, T., Nishimasu, H., Nureki, O., & Toki, S. (2018) · Nature Plants · doi:10.1038/s41477-018-0321-8 · Cited by 185 · see citing works
Development of CRISPR-Cas systems for genome editing and beyond
Zhang, F. (2019) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583519000052 · Cited by 185 · see citing works
Wang, T., Xun, H., Wang, W., Ding, X., Tian, H., Hussain, S., Dong, Q., Li, Y., Cheng, Y., Wang, C., et al. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.779598 · Cited by 185 · see citing works
Bastet, A., Zafirov, D., Giovinazzo, N., Guyon‐Debast, A., Nogué, F., Robaglia, C., & Gallois, J. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13096 · Cited by 182 · see citing works
Chloroplast and mitochondrial DNA editing in plants
Kang, B., Bae, S., Lee, S., Lee, J. S., Kim, A., Lee, H., Baek, G., Seo, H., Kim, J., & Kim, J. (2021) · Nature Plants · doi:10.1038/s41477-021-00943-9 · Cited by 181 · see citing works
Qin, L., Li, J., Wang, Q., Xu, Z., Sun, L., Alariqi, M., Manghwar, H., Wang, G., Li, B., Ding, X. L., et al. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13168 · Cited by 179 · see citing works
MACBETH: Multiplex automated Corynebacterium glutamicum base editing method
Wang, Y., Liu, Y., Liu, J., Guo, Y., Fan, L., Ni, X., Zheng, X., Wang, M., Zheng, P., Sun, J., et al. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.02.016 · Cited by 178 · see citing works
Precision genome editing using cytosine and adenine base editors in mammalian cells
Huang, T. P., Newby, G. A., & Liu, D. R. (2021) · Nature Protocols · doi:10.1038/s41596-020-00450-9 · Cited by 175 · see citing works
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement
Zhang, H., Zhang, J., Lang, Z., Botella, J. R., & Zhu, J. (2017) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2017.1402989 · Cited by 173 · see citing works
CRISPR-Cas9/Cas12a biotechnology and application in bacteria
Yao, R., Liu, D., Jia, X., Zheng, Y., Liu, W. Y., & Xiao, Y. (2018) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2018.09.004 · Cited by 159 · see citing works
Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing
Tang, X. D., Ren, Q., Yang, L., Bao, Y., Zhong, Z., He, Y., Liu, S., Qi, C., Liu, B., Wang, Y., et al. (2018) · Plant Biotechnology Journal · doi:10.1111/pbi.13068 · Cited by 156 · see citing works
Ren, B., Liu, L., Li, S., Kuang, Y., Wang, J., Zhang, D., Zhou, X., Lin, H., & Zhou, H. (2019) · Molecular Plant · doi:10.1016/j.molp.2019.03.010 · Cited by 146 · see citing works
Rasheed, A., Gill, R. A., Hassan, M., Mahmood, A., Qari, S. H., Zaman, Q. U., Ilyas, M., Aamer, M., Batool, M., Li, H., et al. (2021) · Current Issues in Molecular Biology · doi:10.3390/cimb43030135 · Cited by 146 · see citing works
Characterisation of CRISPR mutants targeting genes modulating pectin degradation in ripening tomato
Wang, D., Samsulrizal, N. H., Yan, C., Allcock, N., Craigon, J., Blanco‐Ulate, B., Ortega‐Salazar, I., Marcus, S. E., Bagheri, H., Pérez-Fons, L., et al. (2018) · PLANT PHYSIOLOGY · doi:10.1104/pp.18.01187 · Cited by 144 · see citing works
Can gene editing reduce postharvest waste and loss of fruit, vegetables, and ornamentals?
Shipman, E. N., Yu, J., Zhou, J., Albornoz, K., & Beckles, D. M. (2020) · Horticulture Research · doi:10.1038/s41438-020-00428-4 · Cited by 141 · see citing works
High-efficiency and multiplex adenine base editing in plants using new TadA variants
Yan, D., Ren, B., Liu, L., Yan, F., Li, S., Wang, G., Sun, W., Zhou, X., & Zhou, H. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.02.007 · Cited by 140 · see citing works
MADS-box genes and crop domestication: the jack of all traits
Schilling, S., Pan, S., Kennedy, A., & Melzer, R. (2018) · Journal of Experimental Botany · doi:10.1093/jxb/erx479 · Cited by 139 · see citing works
Precise gene replacement in rice by RNA transcript-templated homologous recombination
Li, S., Li, J., He, Y., Xu, M., Zhang, J., Du, W., Zhao, Y., & Xia, L. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0065-7 · Cited by 137 · see citing works
Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor
Wang, L., Xue, W., Yan, L., Li, X., Wei, J., Chen, M., Wu, J., Yang, B., Yang, L. C., & Chen, J. (2017) · Cell Research · doi:10.1038/cr.2017.111 · Cited by 136 · see citing works
Construct design for CRISPR/Cas-based genome editing in plants
Hassan, M. M., Zhang, Y., Yuan, G., De, K., Chen, J., Muchero, W., Tuskan, G. A., Qi, Y., & Yang, X. (2021) · Trends in Plant Science · doi:10.1016/j.tplants.2021.06.015 · Cited by 136 · see citing works
Genome Editing in Cereals: Approaches, Applications and Challenges
Ansari, W. A., Chandanshive, S. U., Bhatt, V. D., Nadaf, A. B., Vats, S., Katara, J. L., Sonah, H., & Deshmukh, R. K. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21114040 · Cited by 135 · see citing works
Wang, Y., Zafar, N., Ali, Q., Manghwar, H., Wang, G., Yu, L., Ding, X. L., Ding, F., Hong, N., Wang, G., et al. (2022) · Cells · doi:10.3390/cells11233928 · Cited by 135 · see citing works
Mutation Breeding in Tomato: Advances, Applicability and Challenges
Chaudhary, J., Alisha, A., Bhatt, V. D., Chandanshive, S., Kumar, N., Mir, Z. A., Kumar, A., Yadav, S. K., Shivaraj, S. M., Sonah, H., et al. (2019) · Plants · doi:10.3390/plants8050128 · Cited by 134 · see citing works
Kumlehn, J., Pietralla, J., Hensel, G., Pacher, M., & Puchta, H. (2018) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12734 · Cited by 134 · see citing works
Evolution of Alternative Adaptive Immune Systems in Vertebrates
Boehm, T. L. J., Hirano, M., Holland, S. J., Das, S., Schorpp, M., & Cooper, M. D. (2017) · Annual Review of Immunology · doi:10.1146/annurev-immunol-042617-053028 · Cited by 132 · see citing works
Improved plant cytosine base editors with high editing activity, purity, and specificity
Ren, Q., Sretenovic, S., Liu, G., Zhong, Z., Wang, J., Huang, L., Tang, X., Guo, Y., Liu, L., Wu, Y., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13635 · Cited by 131 · see citing works
Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops
Villalobos-López, M. Á., Arroyo-Becerra, A., Quintero-Jiménez, A., & Iturriaga, G. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms231912053 · Cited by 128 · see citing works
CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences
Karlson, C. K. S., Noor, S. N. M., Nolte, N., & Tan, B. C. (2021) · Plants · doi:10.3390/plants10102055 · Cited by 126 · see citing works
Multiplex genome-editing technologies for revolutionizing plant biology and crop improvement
Abdelrahman, M., Wei, Z., Rohila, J. S., & Zhao, K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.721203 · Cited by 125 · see citing works
The Solanum tuberosum GBSSI gene: a target for assessing gene and base editing in tetraploid potato
Veillet, F., Chauvin, L., Kermarrec, M.-P., Sevestre, F., Merrer, M., Terret, Z., Szydlowski, N., Devaux, P., Gallois, J.-L., & Chauvin, J.-E. (2019) · Plant Cell Reports · doi:10.1007/s00299-019-02426-w · Cited by 124 · see citing works
Modrzejewski, D., Hartung, F., Sprink, T., Krause, D., Kohl, C., & Wilhelm, R. (2019) · Environmental Evidence · doi:10.1186/s13750-019-0171-5 · Cited by 124 · see citing works
Bhat, M. A., Mir, R. A., Kumar, V., Shah, A. A., Zargar, S. M., Rahman, S., & Jan, A. T. (2021) · Physiologia Plantarum · doi:10.1111/ppl.13359 · Cited by 124 · see citing works
Base editing in plants: Current status and challenges
Bharat, S. S., Li, S., Li, J., Yan, L., & Xia, L. (2019) · The Crop Journal · doi:10.1016/j.cj.2019.10.002 · Cited by 123 · see citing works
Harnessing CRISPR/Cas systems for programmable transcriptional and post-transcriptional regulation
Mahas, A., Stewart, C. N., & Mahfouz, M. M. (2017) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.11.008 · Cited by 121 · see citing works
Genome editing for plant research and crop improvement
Zhan, X., Lu, Y., Zhu, J., & Botella, J. R. (2020) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13063 · Cited by 121 · see citing works
Advances in Crop Breeding Through Precision Genome Editing
Nerkar, G., Devarumath, S., Purankar, M. V., Kumar, A., Valarmathi, R., Devarumath, R. M., & Appunu, C. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.880195 · Cited by 121 · see citing works
CRISPR/Cas9-mediated noncoding RNA editing in human cancers
Yang, J., Meng, X., Pan, J., Jiang, N., Zhou, C., Wu, Z., & Gong, Z. (2017) · RNA Biology · doi:10.1080/15476286.2017.1391443 · Cited by 119 · see citing works
Present and future prospects for wheat improvement through genome editing and advanced technologies
Li, S., Zhang, C., Li, J., Yan, L., Wang, N., & Xia, L. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100211 · Cited by 118 · see citing works
Liu, X., Qin, R., Li, J., Liao, S., Shan, T., Xu, R., Wu, D., & Wei, P. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13348 · Cited by 116 · see citing works
CRISPR/Cas genome editing in tomato improvement: Advances and applications
Tiwari, J. K., Singh, A. K., & Behera, T. K. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1121209 · Cited by 116 · see citing works
CRISPR/Cas9: Development and Application in Rice Breeding
Romero, F. M., & Gatica-Arias, A. (2019) · Rice Science · doi:10.1016/j.rsci.2019.08.001 · Cited by 114 · see citing works
Genome editing for horticultural crop improvement
Xu, J., Hua, K., & Lang, Z. (2019) · Horticulture Research · doi:10.1038/s41438-019-0196-5 · Cited by 114 · see citing works
Carotenoid biofortification in crop plants: citius, altius, fortius
Zheng, X., Giuliano, G., & Al‐Babili, S. (2020) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2020.158664 · Cited by 113 · see citing works
Genome Editing Technologies for Rice Improvement: Progress, Prospects, and Safety Concerns
Zafar, K., Sedeek, K. E. M., Rao, G. S., Khan, M. Z., Amin, I. U., Kamel, R., Mukhtar, Z., Zafar, M., Mansoor, S., & Mahfouz, M. M. (2020) · Frontiers in Genome Editing · doi:10.3389/fgeed.2020.00005 · Cited by 113 · see citing works
Editing the Genome Without Double-Stranded DNA Breaks
Komor, A. C., Badran, A. H., & Liu, D. R. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00710 · Cited by 112 · see citing works
- Authors
- Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T., & Kondo, A.
- Affiliations
- Kobe University (JP)
- University of Tsukuba (JP)
- Meijo University (JP)
- Corresponding author
- Ezura, Hiroshi
- Nishida, Keiji
- Ariizumi, Tohru
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2017-03-27
- Volume
- 35
- Issue
- 5
- Pages
- 441-443
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2598674316
- Cited by
- 812
- References
- 17
- Citations by year
- 2026: 43 · 2025: 71 · 2024: 82 · 2023: 83 · 2022: 117 · 2021: 113 · 2020: 107 · 2019: 97 · 2018: 62 · 2017: 37
- FWCI
- 33.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (15K18647, 16K14654, 16K07560)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- base editing, CRISPR/Cas9, rice, tomato, point mutagenesis, herbicide resistance, single guide RNA, crop improvement
- MeSH terms
- Gene Editing, Cytidine Deaminase, Recombinant Fusion Proteins, Oryza, Mutagenesis, Site-Directed, Genes, Plant, Point Mutation, Solanum lycopersicum, DNA, Plant, Base Pairing, Plants, Genetically Modified, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Associated Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review