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Cited by 109

The DOI matches its Crossref record; 148 of the 148 references it cites were found in a registry or an index.

Transient and stable vector transfection: Pitfalls, off-target effects, artifacts

Stepanenko, Aleksei A., Heng, Henry H.·2017·Mutation Research/Reviews in Mutation Research
DOI 10.1016/j.mrrev.2017.05.002
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Abstract

Transient and stable vector transfections have played important roles in illustrating the function of specific genes/proteins. The general assumption is that such a platform could effectively link a given gene/protein to gained phenotypes, revealing the mechanism of how a gene works. However, in reality, increased studies have surprisingly noticed some unexpected results. In this review, we demonstrate that an assumption that empty vector-transfected cells preserve the cytogenetic and phenotypic characteristics, and represent the adequate control in transfection experiments is not universally valid. A DNA vector, a transfection reagent, expression of an antibiotic resistance (trans)gene, expression of a reporter (trans)gene, and selection by acute/chronic antibiotic treatment may evoke cellular responses that affect the biochemical processes under investigation. We exemplify a number of studies, which reported obvious genomic, transcriptomic and phenotypic changes of tumor cells after transient/stable transfection of an empty vector. To further address the common mechanisms of these unexpected findings, we will apply the genome theory of somatic evolution to explain stress-mediated system dynamics and the limitations of predicting the system behavior solely based on targeted genes. We conceptualize that the diverse experimental manipulations (e.g., transgene overexpression, gene knock out/down, chemical treatments, acute changes in culture conditions, etc.) may act as a syste…

Overview

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Crossref · 10.1016/j.mrrev.2017.05.002

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148 of 148 references verified0 citation errors · 0 not checkable

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Citations

Cites

Research works printed but not linked: 1

  1. Horne, 2015, Genome chaos, chromothripsis and cancer evolution, J Cancer Stud Ther., 1, 1

Cited by

See all 109

Metadata

Authors
Stepanenko, A. A., & Heng, H. H.
Venue
Mutation Research/Reviews in Mutation Research
Publisher
Elsevier BV
Published
2017-07
Volume
773
Pages
91-103
ISSN
1383-5742
DOI
10.1016/j.mrrev.2017.05.002
Cited by
109
References
148
Language
en
Licence
  • www.elsevier.com/tdm/userlicense/1.0/ (tdm)
  • www.elsevier.com/legal/tdmrep-license (tdm)

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.