Nanosized CaP-silk fibroin-PCL-PEG-PCL/PCL based bilayer membranes for guided bone regeneration
Open accessAbstract
Guided bone regeneration (GBR) concept has been developed to prevent the formation of non-functional scar tissue layer on defect site by undertaking barrier role. In this study, a new bilayer membrane which consisted of one layer of electrospun silk fibroin/PCL-PEG-PCL incorporating nanocalcium phosphate (SPCA)1 and one layer of PCL membrane was developed for GBR. To improve the osteoconductivity of membranes, nanosized calcium phosphate particles synthesized by Flame Spray Pyrolysis method were incorporated into membranes at 10% (wt) (SPCA10) and 20% (wt) (SPCA20) of the polymer content. The structural and chemical analyses revealed the well-integrated two layers of membranes with a total thickness of ca 100μm. In the regenerative layer, the highly porous mesh structure had a thickness of 12.6μm with randomly oriented fibers having diameters around 760nm, and nanoparticles dispersed homogenously. The mechanical test results showed remarkable improvement on the tensile strength of membranes with incorporation of nanoparticles. Higher water affinity of nanoCaP included membranes was proved by lower contact angle values and higher percent water uptake capacity. Biomineralization assay revealed that nucleation and growth of apatites around fibers of SPCA10 and SPCA20 were apparent while on SPCA0 apatite minerals were barely detected after 10days. Human dental pulp stem cells (DPSC) were seeded on electrospun layer of the bilayer membranes for biocompatibility and osteo-compatibility study. Increasing nanoCaP amount resulted in higher cell adhesion, proliferation, ALP activity and calcium deposition on membranes. These overall results confirmed the biocompatibility and potential applicability of proposed membranes for GBR treatments.
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When collagen scaffolds are seeded with cells, the rate of cell proliferation is improved.
“When collagen scaffolds are seeded with cells, the rate of cell proliferation is improved [51].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [51]
Cites
1 retracted
Selvakumar, M., Srivastava, P., Pawar, H. S., Francis, N. K., Das, B., Sathishkumar, G., Subramanian, B., Jaganathan, S. K., George, G., Anandhan, S., et al. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b11723 · Cited by 39 · see citing works
RetractedHow useful is SBF in predicting in vivo bone bioactivity?
Kokubo, T., & Takadama, H. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.01.017 · Cited by 9,578 · see citing works
Materials Fabrication from Bombyx mori Silk Fibroin
Rockwood, D., Preda, R. C., Yucel, T., Wang, X.-Q., Lovett, M. L., & Kaplan, D. (2011) · Nature Protocols · doi:10.1038/nprot.2011.379 · Cited by 2,856 · see citing works
Guided Bone Regeneration: biological principle and therapeutic applications
Retzepi, M., & Donos, N. (2010) · Clinical Oral Implants Research · doi:10.1111/j.1600-0501.2010.01922.x · Cited by 660 · see citing works
Guided bone regeneration membrane made of polycaprolactone/calcium carbonate composite nano-fibers
Fujihara, K., Kotaki, M., & Ramakrishna, S. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.014 · Cited by 606 · see citing works
Mechanisms of Guided Bone Regeneration: A Review
Liu, J., & Kerns, D. G. (2014) · The Open Dentistry Journal · doi:10.2174/1874210601408010056 · Cited by 427 · see citing works
Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration
Kim, K.-H., Jeong, L., Park, H.-N., Shin, S., Park, W. H., Lee, S., Kim, T., Park, Y., Seol, Y., Lee, Y., et al. (2005) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2005.06.033 · Cited by 340 · see citing works
Dental Pulp Stem Cells: A Promising Tool for Bone Regeneration
d’Aquino, R., Papaccio, G., Laino, G., & Graziano, A. (2008) · Stem Cell Reviews and Reports · doi:10.1007/s12015-008-9013-5 · Cited by 310 · see citing works
Xue, J., Niu, Y., Gong, M., Shi, R., Chen, D., Zhang, L., & LVOV, Y. M. (2015) · ACS Nano · doi:10.1021/nn506255e · Cited by 307 · see citing works
Calcium phosphate nanoparticles in biomineralization and biomaterials
Cai, Y., & Tang, R. (2008) · Journal of Materials Chemistry · doi:10.1039/b805407j · Cited by 299 · see citing works
Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application
Yang, F., Both, S. K., Yang, X., et al. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.05.023 · Cited by 294 · see citing works
Liao, S., Wang, W., Uo, M., Ohkawa, S., Akasaka, T., Tamura, K., Cui, F., & Watari, F. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.05.050 · Cited by 280 · see citing works
Molecular simulation of protein adsorption and desorption on hydroxyapatite surfaces
Shen, J., Wu, T., Wang, Q., & Pan, H. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.10.016 · Cited by 272 · see citing works
Kim, H., Himeno, T., Kawashita, M., et al. (2004) · Journal of The Royal Society Interface · doi:10.1098/rsif.2004.0003 · Cited by 267 · see citing works
Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., & Supaphol, P. (2005) · Macromolecular Bioscience · doi:10.1002/mabi.200500150 · Cited by 254 · see citing works
Surface properties and biocompatibility of solvent-cast poly[ε-caprolactone] films
Tang, Z. G., Black, R. A., Curran, J. M., Hunt, J. A., Rhodes, N. P., & Williams, D. F. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.12.003 · Cited by 214 · see citing works
Dental Pulp Stem Cells: A Promising Tool for Bone Regeneration
Graziano, A., d’Aquino, R., Laino, G., & Papaccio, G. (2008) · Stem Cell Reviews and Reports · doi:10.1007/s12015-008-9015-3 · Cited by 198 · see citing works
Kikuchi, M., Koyama, Y., Yamada, T., Imamura, Y., Okada, T., Shirahama, N., Akita, K., Takakuda, K., & Tanaka, J. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.02.001 · Cited by 195 · see citing works
Fluoro-apatite and Calcium Phosphate Nanoparticles by Flame Synthesis
Loher, S. F., Stark, W. J., Maciejewski, M., et al. (2004) · Chemistry of Materials · doi:10.1021/cm048776c · Cited by 193 · see citing works
Morphology-Based Prediction of Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells
Matsuoka, F., Takeuchi, I., Agata, H., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0055082 · Cited by 165 · see citing works
Liu, C. B., Gong, C. Y., Huang, M. J., Wang, J. W., Pan, Y. F., Zhang, Y. D., Li, G. Z., Gou, M. L., Wang, K., Tu, M. J., et al. (2007) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30858 · Cited by 162 · see citing works
Li, D., Sun, H., Jiang, L., Zhang, K., Liu, W., Zhu, Y., Fangteng, J., Shi, C., Zhao, L., Sun, H., et al. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am5017792 · Cited by 135 · see citing works
Freeze gelated porous membranes for periodontal tissue regeneration
Qasim, S. S. B., Delaine‐Smith, R. M., Fey, T., et al. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.05.001 · Cited by 123 · see citing works
Jones, D. S., Djokic, J., McCoy, C. P., et al. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00158-8 · Cited by 100 · see citing works
Aligned silk-based 3-D architectures for contact guidance in tissue engineering
Oliveira, A. L., Sun, L., Kim, H. J., et al. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.12.015 · Cited by 93 · see citing works
Phipps, M. C., Clem, W. C., Catledge, S. A., Xu, Y., Hennessy, K. M., Thomas, V., Jablonsky, M. J., Chowdhury, S., Stanishevsky, A. V., Vohra, Y. K., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016813 · Cited by 92 · see citing works
Shi, R., Xue, J., He, M., Chen, D., Zhang, L., & Tian, W. (2014) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2014.07.017 · Cited by 73 · see citing works
A biocomposite of collagen nanofibers and nanohydroxyapatite for bone regeneration
Ribeiro, N., Sousa, S. R., van Blitterswijk, C. A., Moroni, L., & Monteiro, F. J. (2014) · Biofabrication · doi:10.1088/1758-5082/6/3/035015 · Cited by 66 · see citing works
Hild, N., Schneider, O., Mohn, D., Luechinger, N. A., Koehler, F. M., Hofmann, S., Vetsch, J. R., Thimm, B. W., Müller, R., & Stark, W. J. (2010) · Nanoscale · doi:10.1039/c0nr00615g · Cited by 66 · see citing works
Membranes for guided tissue and bone regeneration
Zhang, Y., Zhang, X., Shi, B., et al. (2013) · Annals of Oral and Maxillofacial Surgery · doi:10.13172/2052-7837-1-1-451 · Cited by 64 · see citing works
Li, J., Zuo, Y., Xianmiao, C., et al. (2008) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-008-3664-2 · Cited by 62 · see citing works
Kim, J.-Y., Yang, B., Ahn, J.-H., Park, S. O., & Shim, H.-W. (2014) · The Journal of Advanced Prosthodontics · doi:10.4047/jap.2014.6.6.539 · Cited by 62 · see citing works
Intracellular precipitation of hydroxyapatite mineral and implications for pathologic calcification
Azari, F., Vali, H., Guerquin‐Kern, J., Wu, T., Croisy, A., Sears, S. K., Tabrizian, M., & McKee, M. D. (2008) · Journal of Structural Biology · doi:10.1016/j.jsb.2008.03.003 · Cited by 57 · see citing works
Fu, S., Wang, X. H., Guo, G. F., Shi, S., Fan, M., Liang, H., Luo, F., & Qian, Z. Y. (2011) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31788 · Cited by 51 · see citing works
Ataol, S., Tezcaner, A., Duygulu, Ö., Keskin, D. S., & Machin, N. E. (2015) · Journal of Nanoparticle Research · doi:10.1007/s11051-015-2901-0 · Cited by 39 · see citing works
Self-citationHu, H., Lee, S.-Y., Chen, C., Yang, Y., & Yang, J. (2012) · Polymer Engineering and Science · doi:10.1002/pen.23329 · Cited by 24 · see citing works
Lee, M., Ko, Y., Lee, J. B., et al. (2014) · Macromolecular Research · doi:10.1007/s13233-014-2096-8 · Cited by 22 · see citing works
Allaf, R. M., Rivero, I. V., Abidi, N., et al. (2013) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.32915 · Cited by 21 · see citing works
Son, J., Kim, S.-G., Jin, S.-C., et al. (2011) · Biotechnology Letters · doi:10.1007/s10529-011-0819-x · Cited by 13 · see citing works
Hild, N., Fuhrer, R., Mohn, D., et al. (2012) · Biomedical Materials · doi:10.1088/1748-6041/7/5/054103 · Cited by 7 · see citing works
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Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
de Souza, J. R., Cardoso, L. M., de Toledo, P. T. A., Rahimnejad, M., Kito, L. T., Thim, G. P., Campos, T. M. B., Borges, A. L. S., & Bottino, M. C. (2024) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.35406 · Cited by 30 · see citing works
- Authors
- Türkkan, S., Pazarçeviren, A. E., Keskin, D., Machin, N. E., Duygulu, Ö., & Tezcaner, A.
- Affiliations
- Middle East Technical University (TR)
- Atilim University (TR)
- TÜBİTAK Marmara Research Center (TR)
- Corresponding author
- Tezcaner, Ayşen
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2017-11
- Volume
- 80
- Pages
- 484-493
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2721730800
- Cited by
- 75
- References
- 40
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 7 · 2023: 5 · 2022: 12 · 2021: 11 · 2020: 15 · 2019: 8 · 2018: 6
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- OpenMETU (Middle East Technical University) (submittedVersion, cc-by-nc-nd)
- Funders
- METU (BAP-07-02-2013-00)
- Scientific and Technological Research Council of Turkey (106M232)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- guided bone regeneration, bilayer membranes, silk fibroin, polycaprolactone, PEG, calcium phosphate nanoparticles, flame spray pyrolysis, electrospun membranes, osteoconductivity, biomineralization, dental pulp stem cells, osteogenic differentiation, tensile strength, water absorption, biocompatibility
- MeSH terms
- Biocompatible Materials, Bone Regeneration, Calcium Phosphates, Fibroins, Humans, Polyesters, Polyethylene Glycols, Nanostructures
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