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Cited by 80Lymphatic System and DiseasesBarrier Structure and Function StudiesLymphatic Disorders and Treatments

Effect of cyclic AMP on barrier function of human lymphatic microvascular tubes

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Price, Gavrielle M., Chrobak, Kenneth M., Tien, Joe·2008·Microvascular Research
DOI 10.1016/j.mvr.2008.02.003PMC PMC2574545
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Abstract

This work examines the effect of cyclic AMP (cAMP) on the in vitro barrier function of tubes of human dermal lymphatic microvascular endothelial cells (LECs). Under baseline conditions, the barrier function of LEC tubes was weak, with diffusional permeability coefficients to bovine serum albumin and 10 kDa dextran of 1.4(-0.6)(+0.9)x10(-6) cm/s and 1.7(-0.5)(+0.8)x10(-6) cm/s (geometric mean+/-95% CI), respectively, and 1.2+/-0.5 (mean+/-95% CI) focal leaks per mm. Exposure to low concentrations (3 microM) of a cell-permeant analog of cAMP did not alter the barrier function. Exposure to higher concentrations (80 and 400 microM) and/or the phosphodiesterase inhibitor Ro-20-1724 (20 microM) lowered permeabilities and the number of focal leaks, and increased the selectivity of the barrier. Decreased permeabilities were accompanied by an increase in continuous VE-cadherin staining at cell-cell borders. Exposure to 1 mM 2',5'-dideoxyadenosine, an inhibitor of adenylate cyclase, did not increase permeabilities. LECs expressed the lymphatic-specific master transcription factor Prox-1, regardless of whether barrier function was weak or strong. Our results indicate that the permeability of LEC tubes in vitro responds to cAMP in a manner similar to that well-described for the permeability of blood microvessels.

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  • The permeability of initial lymphatics has not been systematically measured but is estimated to be three- to tenfold higher than that of collecting LVs.

    “The permeability of initial lymphatics has not been systematically measured but is estimated to be three- to tenfold higher than that of collecting LVs (31, 35), reflecting the functional differences between button and zipper junctions.”

    From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [35]

    The Introduction says quantitative permeability of lymphatic microvessels is poorly known because initial lymphatic capillaries are difficult to cannulate. It does not give a three- to tenfold estimate or compare initial with collecting lymphatic vessels; the Results instead measure cultured LEC tubes. (LLM, conf=0.84)

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Research works printed but not linked: 1

  1. Taylor AE, Granger DN. Exchange of macromolecules across the microcirculation. In: Renkin EM, Michel CC, editors. Handbook of Physiology; Section 2: The Cardiovascular System. American Physiological Society; Bethesda, MD: 1984. pp. 467–520. [Google Scholar]

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Authors
Price, G. M., Chrobak, K. M., & Tien, J.
Affiliations
Boston University (US)
Corresponding author
Tien, Joe
ORCID
Tien, Joe
Venue
Microvascular Research
Publisher
Elsevier BV
Published
2008-05
Volume
76
Issue
1
Pages
46-51
ISSN
0026-2862 (ISSN-L)
DOI
10.1016/j.mvr.2008.02.003
PMC
PMC2574545
OpenAlex
W2135431568
Cited by
80
References
53
Citations by year
2026: 1 · 2025: 2 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 2 · 2020: 6 · 2019: 6 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 5 · 2014: 4 · 2013: 2 · 2012: 3
FWCI
3.88
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Microvascular Research (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Biomedical Imaging and Bioengineering (EB002228, EB005792, R21EB002228, R01EB005792)
Topics
  • Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Surgery › Lymphatic Disorders and Treatments
Keywords
cyclic AMP, lymphatic endothelial cells, endothelial permeability, phosphodiesterase inhibition, Ro 20-1724, VE-cadherin, Prox1, adenylate cyclase inhibition, dextran permeability
MeSH terms
Adenylyl Cyclase Inhibitors, Prospero-Related Homeobox 1 Protein, Podoplanin, Cadherin 5, Cyclic AMP, Capillary Permeability, Cell Nucleus, Cells, Cultured, Dextrans, Bucladesine, Endothelium, Lymphatic, Enzyme Inhibitors, Humans, Membrane Glycoproteins, Membrane Proteins, Phosphoproteins, 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone, Serum Albumin, Bovine, Antigens, CD, Cadherins, Dideoxyadenosine, Homeodomain Proteins, Tight Junctions, Platelet Endothelial Cell Adhesion Molecule-1, Adherens Junctions, Tissue Engineering, Tumor Suppressor Proteins, Endothelial Cells, Zonula Occludens-1 Protein

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