Sustained astrocytic clusterin expression improves remodeling after brain ischemia
Open accessAbstract
Clusterin is a glycoprotein highly expressed in response to tissue injury. Using clusterin-deficient (Clu-/-) mice, we investigated the role of clusterin after permanent middle cerebral artery occlusion (MCAO). In wild-type (WT) mice, clusterin mRNA displayed a sustained increase in the peri-infarct area from 14 to 30 days post-MCAO. Clusterin transcript was still present up to 90 days post-ischemia in astrocytes surrounding the core infarct. Western blot analysis also revealed an increase of clusterin in the ischemic hemisphere of WT mice, which culminates up to 30 days post-MCAO. Concomitantly, a worse structural restoration and higher number of GFAP-reactive astrocytes in the vicinity of the infarct scar were observed in Clu-/- as compared to WT mice. These findings go beyond previous data supporting a neuroprotective role of clusterin in early ischemic events in that they demonstrate that this glycoprotein plays a central role in the remodeling of ischemic damage.
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In wild-type mice, CLU enhanced recovery after brain ischemia, accompanied by elevated astrocytic CLU mRNA in the peri-infarct region for up to three months.
“CLU improved healing after brain ischemia in wild-type mice where there was an increase in CLU mRNA in astrocytes in the peri-infarct area up to three months post-ischemia”
From A potential endophenotype for Alzheimer's disease: cerebrospinal fluid clusterin (2016) · cites this paper as Imhof, et al., 2006
Mice lacking CLU had significantly less healing after ischemia.
“CLU−/− mice showed a significant reduction in healing post-ischemia (Imhof, et al., 2006).”
From A potential endophenotype for Alzheimer's disease: cerebrospinal fluid clusterin (2016) · cites this paper as Imhof, et al., 2006
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“CLU improved healing after brain ischemia inwild-typemice where there was an increase in CLU messenger RNA in astrocytes in the peri-infarct area up to 3 months postischemia, and CLU / mice showed a significant reduction in healing postischemia (Imhof et al., 2006).”
Background
- Authors
- Imhof, A., Charnay, Y., Vallet, P. G., Aronow, B., Kovari, E., French, L. E., Bouras, C., & Giannakopoulos, P.
- Affiliations
- University Hospital of Geneva (CH)
- Cincinnati Children's Hospital Medical Center (US)
- Venue
- Neurobiology of Disease
- Publisher
- Elsevier BV
- Published
- 2006-05
- Volume
- 22
- Issue
- 2
- Pages
- 274-283
- ISSN
- 0969-9961 (ISSN-L)
- OpenAlex
- W2043132438
- Cited by
- 73
- References
- 61
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 7 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 7 · 2014: 3 · 2013: 3 · 2012: 3
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- green
- doaj.org
- Licence
- Locations
- Neurobiology of Disease (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Health Sciences › Medicine › Oncology › Clusterin in disease pathology
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
- Keywords
- clusterin, astrocytes, cerebral ischemia, middle cerebral artery occlusion, peri-infarct zone, glial fibrillary acidic protein, infarct scar, neuroprotection, glycoproteins
- MeSH terms
- Animals, Astrocytes, Brain, Brain Ischemia, Disease Models, Animal, Glial Fibrillary Acidic Protein, Gliosis, Male, Mice, Inbred C57BL, Neuronal Plasticity, RNA, Messenger, Wound Healing, Up-Regulation, Mice, Knockout, Infarction, Middle Cerebral Artery, Brain Infarction, Clusterin, Mice
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