Next-generation DNA sequencing techniques
Open accessAbstract
Next-generation high-throughput DNA sequencing techniques are opening fascinating opportunities in the life sciences. Novel fields and applications in biology and medicine are becoming a reality, beyond the genomic sequencing which was original development goal and application. Serving as examples are: personal genomics with detailed analysis of individual genome stretches; precise analysis of RNA transcripts for gene expression, surpassing and replacing in several respects analysis by various microarray platforms, for instance in reliable and precise quantification of transcripts and as a tool for identification and analysis of DNA regions interacting with regulatory proteins in functional regulation of gene expression. The next-generation sequencing technologies offer novel and rapid ways for genome-wide characterisation and profiling of mRNAs, small RNAs, transcription factor regions, structure of chromatin and DNA methylation patterns, microbiology and metagenomics. In this article, development of commercial sequencing devices is reviewed and some European contributions to the field are mentioned. Presently commercially available very high-throughput DNA sequencing platforms, as well as techniques under development, are described and their applications in bio-medical fields discussed.
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Particularly in the last 25 years, there has been an ever increasing number of ALE experiments with Escherichia coli and Saccharomyces cerevisiae being the most prominent organisms under investigation.
“however, particularly in the last 25 years, there has been an ever increasing number of such experiments with Escherichia coli and Saccharomyces cerevisiae being the most prominent organisms under investigation [26-28].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [28]
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- Authors
- Ansorge, W. J.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Ansorge, Wilhelm J.
- Venue
- New Biotechnology
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 25
- Issue
- 4
- Pages
- 195-203
- ISSN
- 1871-6784 (ISSN-L)
- OpenAlex
- W2058839892
- Cited by
- 993
- References
- 26
- Citations by year
- 2026: 3 · 2025: 27 · 2024: 22 · 2023: 28 · 2022: 27 · 2021: 55 · 2020: 64 · 2019: 56 · 2018: 59 · 2017: 51 · 2016: 61 · 2015: 80 · 2014: 74 · 2013: 92 · 2012: 78
- FWCI
- 39.25
- Type
- journal article
- Language
- en
- Open access
- green
- infoscience.epfl.ch
- Licence
- Locations
- New Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomics and Rare Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genetics, Bioinformatics, and Biomedical Research
- Keywords
- next-generation sequencing, sequencing platforms, personal genomics, RNA sequencing, gene expression profiling, transcript quantification, protein-DNA interactions, metagenomics, DNA methylation, chromatin structure, small RNA, transcription factors
- MeSH terms
- Chromosome Mapping, Sequence Alignment, Sequence Analysis, DNA
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