Morphological transformation and proliferation of rat astrocytes as induced by sulfated polysaccharides from the sea cucumber Stichopus japonicus
Abstract
In this report, we demonstrate that the sulfated polysaccharide, Haishen (HS), which was isolated from the body wall of the sea cucumber Stichopus japonicus can induce morphological transformation and proliferation of astrocytes in vitro when combined with basic fibroblast growth factor 2 (FGF-2). Cell morphology showed no change when induced by HS or FGF-2 alone. However, combinational treatment of HS and FGF-2 promoted transformation of normal astrocyte into a stella morphology (stellation), along with an increase in the expression and rearrangement of glial fibrillary acidic protein (GFAP). Further analysis of HS- and FGF-2-treated cells indicated a reduced percentage of cells in the G0/G1 phase, whereas the cell proliferation index (S phase) was increased. The proportion of 5-bromo-2-deoxyuridine (BrdU)-positive cells increased in response to the combination of HS and FGF-2. With respect to cell cycle signaling, immunoblotting assay demonstrated an accumulation of Cyclin D1. These observations suggest that HS may play a role in astrocyte morphological transformation and proliferation, and this activation requires a synergism with FGF-2.
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Polysaccharides from marine animals demonstrate diverse biological properties, including anticoagulant, antithrombotic, antioxidative, neuroprotective, and antiviral activity.
“These compounds demonstrate diverse biological properties, including anticoagulant and antitrombotic [131,132,133], antioxidative [134], neuroprotective [135,136], and antiviral activity as well [8,137].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [136]
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- Authors
- Sheng, X., Zhang, N., Song, S., Li, M., Liang, H., Zhang, Y., Wang, Y., & Ji, A.
- Affiliations
- Shandong University (CN)
- Shandong University of Technology (CN)
- Corresponding author
- Ji, Aiguo
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 503
- Issue
- 1
- Pages
- 37-42
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W1969982861
- Cited by
- 11
- References
- 25
- Citations by year
- 2023: 2 · 2021: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 1
- FWCI
- 1.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30873198)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- sulfated polysaccharides, sea cucumbers, Apostichopus japonicus, astrocytes, stellate astrocytes, cell proliferation, glial fibrillary acidic protein, cell cycle, cyclin D1, synergistic effect, cell cycle signaling
- MeSH terms
- Animals, Animals, Newborn, Astrocytes, Cell Count, Cell Cycle, Cells, Cultured, Cerebral Cortex, Dose-Response Relationship, Drug, Gene Expression Regulation, Glial Fibrillary Acidic Protein, Polysaccharides, Sea Cucumbers, Time Factors, Fibroblast Growth Factor 2, Rats, Wistar, Cyclin D1, Cell Proliferation, Rats
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