Enhancing effect of a sea cucumber Stichopus japonicus sulfated polysaccharide on neurosphere formation in vitro
Abstract
Neural stem/progenitor cells (NSPCs) exhibit therapeutic potential in neuronal diseases. Previously, we reported that a sulfated polysaccharide (HS) from the sea cucumber Stichopus japonicus increased the proliferation of NSPCs. Since the formation of neurospheres is related with NSPCs proliferation, we investigated the mechanism leading to neurosphere formation with and without HS. The results showed that HS significantly promoted neurosphere formation in a dose-dependent manner at concentrations between 2 and 8 μg/ml. Cell cycle analysis showed that HS increased the percentage of cells in S phase by 2.8-fold, as compared with the control. On the other hand, we observed a significantly rapid aggregation of NSPCs, resulting in formation of neurospheres as early as 2 h after HS treatment. However, the aggregation was not caused by chemotactic migration of NSPCs, as evidenced by the transwell chamber assay. Furthermore, the effect of HS on NSPCs was similar to the tumor necrosis factor-α (TNF-α) that activated nuclear factor NF-κB. Thus, we demonstrated that HS was able to promote cell proliferation and aggregation of NSPCs which could lead to the formation of neurospheres, and suggested that HS can serve as an adjuvant for promoting proliferation of NSPCs and formation of neurospheres.
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Polysaccharides from marine animals demonstrate diverse biological properties, including anticoagulant, antithrombotic, antioxidative, neuroprotective, and antiviral activity.
“These compounds demonstrate diverse biological properties, including anticoagulant and antitrombotic [131,132,133], antioxidative [134], neuroprotective [135,136], and antiviral activity as well [8,137].”
From Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects (2013) · cites this paper as [135]
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Anticancer and Cancer Preventive Properties of Marine Polysaccharides: Some Results and Prospects
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Self-citation
- Authors
- Zhang, Y., Song, S., Liang, H., Wang, Y., Wang, W., & Ji, A.
- Affiliations
- Shandong University of Technology (CN)
- Shandong University (CN)
- Corresponding author
- Ji, Aiguo
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2010-10
- Volume
- 110
- Issue
- 4
- Pages
- 479-486
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W2008926759
- Cited by
- 20
- References
- 31
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 2 · 2020: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Foundation for the National Institutes of Health
- National Natural Science Foundation of China
- National Institutes of Natural Sciences
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Echinoderm biology and ecology
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- Apostichopus japonicus, sulfated polysaccharides, sea cucumber polysaccharides, neurosphere formation, neural stem/progenitor cells, cell proliferation, cell cycle, cell aggregation, TNF-α, NF-κB, neuronal diseases, cell migration
- MeSH terms
- Animals, Neurons, Polysaccharides, Sea Cucumbers, Stem Cells, Sulfates, Cell Lineage
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