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Cited by 20Axon Guidance and Neuronal SignalingNeurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology Research

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Serotonin 2A receptor regulates microtubule assembly and induces dynamics of dendritic growth cones in rat cortical neurons in vitro

Ohtani, Akiko, Kozono, Naoki, Senzaki, Kouji, Shiga, Takashi·2014·Neuroscience Research
DOI 10.1016/j.neures.2014.03.006
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Abstract

Serotonin (5-HT) regulates the development of cerebral cortex, but 5-HT receptors mediating the effects are poorly understood. We investigated roles of 5-HT2A receptor in dendritic growth cones using dissociation culture of rat cerebral cortex. Neurons at embryonic day 16 were cultured for 4 days and treated with 5-HT2A/2C receptor agonist (DOI) for 4h. DOI increased the size of growth cone periphery which was actin-rich and microtubule-associated protein 2-negative at the dendritic tip. The length increase of the growth cone periphery may be mediated by 5-HT2A receptor, because the 5-HT2A receptor antagonist reversed the effects of DOI. Moreover, the time-lapse analysis demonstrated the increase of morphological dynamics in dendritic growth cones by DOI. Next, to elucidate the mechanisms underlying the actions of 5-HT2A receptor in dendritic growth cones, we examined the cytoskeletal proteins, tyrosinated α-tubulin (Tyr-T; dynamic tubulin) and acetylated α-tubulin (Ace-T; stable tubulin). DOI increased the fluorescence intensity of Tyr-T, while decreased that of Ace-T in the dendritic growth cone periphery. These effects were reversed by the 5-HT2A receptor antagonist, suggesting that 5-HT2A receptor promotes microtubule dynamics. In summary, it was suggested that 5-HT2A receptor induces morphological changes and dynamics of dendritic growth cones through regulation of microtubule assembly.

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Research works printed but not linked: 44

  1. Azmitia, 2001, Modern views on an ancient chemical: serotonin effects on cell proliferation, maturation, and apoptosis, Brain Res. Bull., 56, 413, 10.1016/S0361-9230(01)00614-1 · DOI · Paper page
  2. Barnes, 1999, A review of central 5-HT receptors and their function, Neuropharmacology, 38, 1083, 10.1016/S0028-3908(99)00010-6 · DOI
  3. Bayer, 1991, Cell migration in the rat embryonic neocortex, J. Comp. Neurol., 307, 499, 10.1002/cne.903070312 · DOI
  4. Berry, 1964, Pattern of cell migration during cortical histogenesis, Nature, 203, 591, 10.1038/203591b0 · DOI
  5. Bockaert, 2006, Neuronal 5-HT metabotropic receptor: fine-tuning of their structure, signaling, and roles in synaptic modulation, Cell Tissue Res., 326, 553, 10.1007/s00441-006-0286-1 · DOI
  6. Chubakov, 1986, The effects of serotonin on the morpho-functional development of rat cerebral neocortex in tissue culture, Brain Res., 369, 285, 10.1016/0006-8993(86)90537-8 · DOI
  7. Conde, 2009, Microtubule assembly, organization and dynamics in axon and dendrites, Nat. Rev. Neurosci., 10, 319, 10.1038/nrn2631 · DOI · Paper page
  8. Creppe, 2009, Elongator controls the migration and differentiation of cortical neurons through acetylation of α-tubulin, Cell, 136, 551, 10.1016/j.cell.2008.11.043 · DOI
  9. Crespi, 2010, Further electrochemical and behavioural evidence of a direct relationship between central 5-HT and cytoskeleton in the control of mood, Open Neurol. J., 4, 5, 10.2174/1874205X01004010005 · DOI
  10. Dehmelt, 2004, Actin and microtubules in neurite initiation: are MAPs the missing link?, J. Neurobiol., 58, 18, 10.1002/neu.10284 · DOI
  11. De Lima, 1997, Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of rat cerebral cortex, J. Comp. Neurol., 382, 230, 10.1002/(SICI)1096-9861(19970602)382:2<230::AID-CNE7>3.0.CO;2-4 · DOI
  12. Dent, 2003, Cytoskeletal dynamics and transport in growth cone motility and axon guidance, Neuron, 40, 209, 10.1016/S0896-6273(03)00633-0 · DOI
  13. Dooley, 1997, Serotonin promotes the survival of cortical glutamatergic neurons in vitro, Exp. Neurol., 148, 205, 10.1006/exnr.1997.6633 · DOI
  14. Erck, 2005, A vital role of tubulin-tyrosine-ligase for neuronal organization, Proc. Natl. Acad. Sci. U.S.A., 102, 7853, 10.1073/pnas.0409626102 · DOI
  15. Ferreira, 2010, Serotonin receptor 1A modulates actin dynamics and restricts dendritic growth in hippocampal neurons, Eur. J. Neurosci., 32, 18, 10.1111/j.1460-9568.2010.07283.x · DOI
  16. Gandou, 2010, Neurotensin promotes the dendrite elongation and the dendritic spine maturation of the cerebral cortex in vitro, Neurosci. Res., 66, 246, 10.1016/j.neures.2009.11.007 · DOI
  17. Gaspar, 2003, The developmental role of serotonin: news from mouse molecular genetics, Nat. Rev. Neurosci., 4, 1002, 10.1038/nrn1256 · DOI · Paper page
  18. Geraldo, 2009, Cytoskeletal dynamics in growth-cone steering, J. Cell Sci., 122, 3595, 10.1242/jcs.042309 · DOI
  19. Gu, 2008, Microtubules in dendritic spine development, J. Neurosci., 28, 12120, 10.1523/JNEUROSCI.2509-08.2008 · DOI
  20. Hamada, 1998, Localization of 5-HT2A receptor in rat cerebral cortex and olfactory system revealed by immunohistochemistry using two antibodies raised in rabbit and chicken, Mol. Brain Res., 54, 199, 10.1016/S0169-328X(97)00322-7 · DOI
  21. Harigai, 2011, Differential roles of calcitonin family peptides in the dendrite formation and spinogenesis of the cerebral cortex in vitro, Neuropeptides, 45, 263, 10.1016/j.npep.2011.04.001 · DOI
  22. Hayashi, 2010, Roles of serotonin 5-HT3 receptor in the formation of dendrites and axons in the rat cerebral cortex: an in vitro study, Neurosci. Res., 66, 22, 10.1016/j.neures.2009.09.004 · DOI
  23. Hellendall, 1993, Prenatal expression of 5-HT1C and 5-HT2 receptors in the rat central nervous system, Exp. Neurol., 120, 186, 10.1006/exnr.1993.1054 · DOI
  24. Hu, 2008, Activity-dependent dynamic microtubule invasion of dendritic spines, J. Neurosci., 28, 13094, 10.1523/JNEUROSCI.3074-08.2008 · DOI
  25. Ignacio, 1995, Postnatal migration of neurons and formation of laminae in rat cerebral cortex, Anat. Embryol., 191, 89, 10.1007/BF00186782 · DOI
  26. Jaworski, 2009, Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity, Neuron, 61, 85, 10.1016/j.neuron.2008.11.013 · DOI · Paper page
  27. Kim, 2013, Mice lacking α-tubulin acetyltransferase 1 are viable but display α-tubulin acetylation deficiency and dentate gyrus distortion, J. Biol. Chem., 288, 20334, 10.1074/jbc.M113.464792 · DOI · Paper page
  28. Lauder, 1990, Ontogeny of the serotonergic system in the rat: serotonin as a developmental signal, Ann. N.Y. Acad. Sci., 600, 297, 10.1111/j.1749-6632.1990.tb16891.x · DOI
  29. Lavdas, 1997, Serotonin promotes the differentiation of glutamate neurons in organotypic slice cultures of the developing cerebral cortex, J. Neurosci., 17, 7872, 10.1523/JNEUROSCI.17-20-07872.1997 · DOI
  30. Li, 2012, Receptor-dependent regulation of dendrite formation of noradrenalin and dopamine in non-GABAergic cerebral cortical neurons, Dev. Neurobiol., 73, 370, 10.1002/dneu.22065 · DOI
  31. Li, 1996, Microtubule assembly and turnover in growing axons, J. Neurosci., 16, 531, 10.1523/JNEUROSCI.16-02-00531.1996 · DOI
  32. Li, 2004, Unique expression patterns of 5-HT2A and 5-HT2C receptors in the rat brain during postnatal development: western blot and immunohistochemical analyses, J. Comp. Neurol., 469, 128, 10.1002/cne.11004 · DOI
  33. Lidov, 1982, An immunohistochemical study of serotonin neuron development in the rat: ascending pathways and terminal fields, Brain Res. Bull., 8, 389, 10.1016/0361-9230(82)90077-6 · DOI
  34. Lidov, 1982, Immunohistochemical study of the development of serotonergic neurons in the rat CNS, Brain Res. Bull., 9, 559, 10.1016/0361-9230(82)90164-2 · DOI
  35. Lowery, 2009, The trip of the trip: understanding the growth cone machinery, Nat. Rev. Mol. Cell Biol., 10, 332, 10.1038/nrm2679 · DOI
  36. Marcos, 2009, Tubulin tyrosination is required for the proper organization and pathfinding of the growth cone, PLoS ONE, 4, e5405, 10.1371/journal.pone.0005405 · DOI
  37. Matsukawa, 2003, Synaptic loss following depletion of noradrenalin and/or serotonin in the rat visual cortex: a quantitative electron microscopic study, Neuroscience, 122, 627, 10.1016/j.neuroscience.2003.08.047 · DOI
  38. Miner, 2003, Ultrastructural localization of serotonin 2A receptors in the middle layers of the rat prelimbic prefrontal cortex, Neuroscience, 116, 107, 10.1016/S0306-4522(02)00580-8 · DOI · Paper page
  39. Penzes, 2009, Not just actin? A role for dynamic microtubules in dendritic spines, Neuron, 61, 3, 10.1016/j.neuron.2008.12.018 · DOI
  40. Poulain, 2010, The microtubule network and neuronal morphogenesis: dynamic and coordinated orchestration through multiple players, Mol. Cell Neurosci., 43, 15, 10.1016/j.mcn.2009.07.012 · DOI
  41. Roppongi, 2013, Selective reduction of drebrin and actin in dendritic spines of hippocampal neurons by activation of 5-HT2A receptors, Neurosci. Lett., 547, 76, 10.1016/j.neulet.2013.04.061 · DOI
  42. Wallace, 1983, Development of the serotonergic system in the rat embryo: an immunocytochemical study, Brain Res. Bull., 10, 459, 10.1016/0361-9230(83)90144-2 · DOI
  43. Yoshida, 2011, Subtype specific roles of serotonin receptors in the spine formation of cortical neurons in vitro, Neurosci. Res., 71, 311, 10.1016/j.neures.2011.07.1824 · DOI · Paper page
  44. Zhou, 2004, How actin filaments and microtubules steer growth cones to their targets, J. Neurobiol., 58, 84, 10.1002/neu.10278 · DOI

Cited by

Sorted by citations

  • Psychedelics and Neural Plasticity: Therapeutic Implications

    Grieco, S. F., Castrén, E., Knudsen, G. M., Kwan, A. C., Olson, D. E., Zuo, Y., Holmes, T. C., & Xu, X. (2022) · The Journal of Neuroscience · doi:10.1523/jneurosci.1121-22.2022 · Cited by 82

    “DOI, one of the more readily accessible psychedelics to researchers, has been shown to increase dendritic growth (Persico et al., 2006; Ohtani et al., 2014; Ly, Greb et al., 2018; Ly et al., 2021), synaptic puncta, spine density (Yoshida et al., 2011; Ly et al., 2018), and spine size (Jones et al.,…”

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Metadata

Authors
Ohtani, A., Kozono, N., Senzaki, K., & Shiga, T.
Affiliations
University of Tsukuba (JP)
Corresponding author
Shiga, Takashi
ORCID
Venue
Neuroscience Research
Publisher
Elsevier BV
Published
2014-04
Volume
81-82
Pages
11-20
ISSN
0168-0102 (ISSN-L)
DOI
10.1016/j.neures.2014.03.006
OpenAlex
W2044429840
Cited by
20
References
4
Citations by year
2026: 3 · 2025: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2019: 5 · 2018: 1 · 2017: 1 · 2016: 1
FWCI
0.30
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Neuroscience Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Lupus Research Institute
  • Japan Chemical Industry Association
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
5-HT2A receptor, microtubule dynamics, microtubule assembly, acetylated alpha-tubulin, cortical neurons, dendrite morphogenesis, cortical development
MeSH terms
Animals, Cerebral Cortex, Cytoskeleton, Dendrites, Microtubules, Rats, Wistar, Growth Cones, Receptor, Serotonin, 5-HT2A, Rats, Serotonin 5-HT2 Receptor Agonists

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