Detection of postmortem human cerebellar cortex and white matter pathways using high angular resolution diffusion tractography: a feasibility study
Open accessAbstract
Imaging three-dimensional cerebellar connectivity using diffusion tractography is challenging because of the ubiquitous features of crossing axonal pathways within a folium as well as intersecting pathways from neighboring folia. We applied high-angular resolution diffusion imaging (HARDI) tractography to intact postmortem adult brainstem and cerebellum to examine the 3-dimensional white matter and local gray matter pathways. The middle cerebellar peduncles conveyed fibers from the rostral pons to the lateral and caudal aspects of the cerebellar hemisphere, and from the caudal pons to medial and rostral parts of the cerebellar hemisphere. In the cerebellar cortex, tractography detected tangential coherence superficially in the cerebellar cortex and revealed fibers coursing parallel to the long axis of the folia. These fibers were consistent with the location and direction of parallel fibers in the molecular layer. Crossing with these parallel fibers were tangential fibers running perpendicular to the long axis of the folia, consistent with axons of the cortical interneurons - stellate cells and basket cells. These tangential fibers within the cerebellar cortex were distinct from the fibers linking the cerebellar cortex with the deep cerebellar nuclei and the brainstem. Our results show the potential for HARDI tractography to resolve axonal pathways from different neuronal elements within the cerebellar cortex, and improve our understanding of adult cerebellar neural circuitry and connectivity in both white and gray matter.
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Ex vivo dMRI in cadaveric samples appears ideally suited to the study of normal anatomy because it can be obtained with maximal quality.
“In terms of validation, ex vivo dMRI in cadaveric samples appears ideally suited to the study of normal anatomy as it can be obtained with maximal quality.”
From Superior Longitudinal Fasciculus: A Review of the Anatomical Descriptions With Functional Correlates (2022) · cites this paper as [53]
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Superior Longitudinal Fasciculus: A Review of the Anatomical Descriptions With Functional Correlates
Janelle, F., Iorio-Morin, C., D'amour, S., & Fortin, D. (2022) · Frontiers in Neurology · doi:10.3389/fneur.2022.794618 · Cited by 189 · see citing works
Exploring early human brain development with structural and physiological neuroimaging
Vasung, L., Abaci Turk, E., Ferradal, S. L., Sutin, J., Stout, J. N., Ahtam, B., Lin, P.-Y., & Grant, P. E. (2019) · NeuroImage · doi:10.1016/j.neuroimage.2018.07.041 · Cited by 177 · see citing works
Self-citation
- Authors
- Takahashi, E., Song, J. W., Folkerth, R. D., Grant, P. E., & Schmahmann, J. D.
- Affiliations
- Boston Children's Hospital (US)
- Brigham and Women's Hospital (US)
- Harvard University (US)
- Corresponding author
- Takahashi, Emi
- Venue
- NeuroImage
- Publisher
- Elsevier BV
- Published
- 2013-03
- Volume
- 68
- Pages
- 105-111
- ISSN
- 1053-8119 (ISSN-L)
- PMC
- PMC4393953
- OpenAlex
- W1993569317
- Cited by
- 47
- References
- 2
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 1
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- NeuroImage (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Eunice Shriver Kennedy National Institute of Child Health and Development (NICHD) (R21HD069001)
- National Institute of Mental Health (R01MH06044)
- Biomedical Technology Program of the National Center for Research Resources (NCRR), National Institutes of Health
- NCRR Shared Instrumentation Grant Program (1S10RR023401, 1S10RR019307, 1S10RR023043)
- High-End Instrumentation Grant Program (S10RR016811)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Life Sciences › Neuroscience › Cognitive Neuroscience › Functional Brain Connectivity Studies
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- cerebellar cortex, cerebellar connectivity, middle cerebellar peduncle, parallel fibers, molecular layer, stellate cells, basket cells, cortical interneurons, deep cerebellar nuclei, brainstem, crossing fibers, postmortem brain, cerebellar folia
- MeSH terms
- Adult, Autopsy, Cerebellum, Feasibility Studies, Humans, Nerve Fibers, Myelinated, Neural Pathways, Imaging, Three-Dimensional, Diffusion Tensor Imaging
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