The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Distinct Stages of Myelination Regulated by γ-Secretase and Astrocytes in a Rapidly Myelinating CNS Coculture System
Open accessAbstract
SUMMARY Mechanistic studies of CNS myelination have been hindered by the lack of a rapidly myelinating culture system. Here we describe a versatile CNS coculture method that allows time-lapse microscopy and molecular analysis of distinct stages of myelination. Employing a culture architecture of reaggregated neurons fosters extension of dense beds of axons from purified retinal ganglion cells. Seeding of oligodendrocyte precursor cells on these axons results in differentiation and ensheathment in as few as three days, with generation of compact myelin within six days. This technique enabled: (1) demonstration that oligodendrocytes initiate new myelin segments only during a brief window early in their differentiation, (2) identification of a contribution of astrocytes to the rate of myelin wrapping, and (3) molecular dissection of the role of oligodendrocyte γ-secretase activity in controlling the ensheathment of axons. These insights illustrate the value of this defined system for investigating multiple aspects of CNS myelination.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Cahoy, J. D., Emery, B., Kaushal, A., Foo, L. C., Zamanian, J. L., Christopherson, K. S., Xing, Y., Lubischer, J. L., Krieg, P. A., Krupenko, S. A., et al. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.4178-07.2008 · Cited by 3,129
Self-citationSaura, C. A., Choi, S.-Y., Beglopoulos, V., Malkani, S., Zhang, D., Rao, B. S. S., Chattarji, S., Kelleher, R. J., Kandel, E. R., Duff, K., et al. (2004) · Neuron · doi:10.1016/s0896-6273(04)00182-5 · Cited by 757
Functional Genomic Analysis of Oligodendrocyte Differentiation
Dugas, J. C., Tai, Y. C., Speed, T. P., Ngai, J., & Barres, B. A. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.2572-06.2006 · Cited by 324
Self-citationNotch1 control of oligodendrocyte differentiation in the spinal cord
Genoud, S., Lappe-Siefke, C., Goebbels, S., Radtke, F., Aguet, M., Scherer, S. S., Suter, U., Nave, K.-A., & Mantei, N. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200202002 · Cited by 211
Gow, A., Friedrich, V. L., & Lazzarini, R. A. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.119.3.605 · Cited by 147
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Barres, B. A. (2008) · Neuron · doi:10.1016/j.neuron.2008.10.013 · Cited by 1,352
Self-citationOligodendrocytes: biology and pathology
Bradl, M., & Lassmann, H. (2010) · Acta Neuropathologica · doi:10.1007/s00401-009-0601-5 · Cited by 933
Astrocytes and disease: a neurodevelopmental perspective
Molofsky, A. V., Krenick, R., Ullian, E., Tsai, H.-H., Deneen, B., Richardson, W. D., Barres, B. A., & Rowitch, D. H. (2012) · Genes & Development · doi:10.1101/gad.188326.112 · Cited by 682
Self-citationRegulation of oligodendrocyte differentiation and myelination
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Metadata
- Authors
- Watkins, T. A., Emery, B., Mulinyawe, S., & Barres, B. A.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2008-11
- Volume
- 60
- Issue
- 4
- Pages
- 555-569
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2057135010
- Cited by
- 319
- References
- 6
- FWCI
- 4.72
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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