Myelin basic protein gene contains separate enhancers for oligodendrocyte and Schwann cell expression
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The DNA sequence between position +36 and -1907 of the murine myelin basic protein gene contains the enhancer and promoter elements necessary for abundant and cell specific expression in transgenic mice. Surprisingly, the pattern of expression promoted by this DNA fragment is a subset of that exhibited by the endogenous myelin basic protein (MBP) gene. Fusion genes prepared with this promoter/enhancer and a Lac Z reporter gene are expressed only in oligodendrocytes and not in Schwann cells, whereas the endogenous MBP gene is expressed in both cell types. The level of transgene expression measured by nuclear run-on experiments is very substantial and rivals that of the endogenous MBP gene. Furthermore, this 1.9-kb DNA fragment directs transcription on the same (or very similar) developmental schedule as the endogenous gene. These results indicate that the MBP promoter/enhancer sequences are at least tripartite: a core promoter, the oligodendrocyte enhancer elements, and a third component that either expands the specificity of the oligodendrocyte enhancer to include Schwann cells or acts independently to specifically stimulate transcription in Schwann cells.
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Both Mbp promoters were selected as they had been shown to drive transgenes in the oligodendrocyte lineage in transgenic mouse lines generated by pronucleus injections.
“Like the 2,3-cyclic nucleotide 3-phosphodiesterase promoter both Mbp promoters were selected as they had been shown to drive transgenes in the oligodendrocyte lineage in transgenic mouse lines generated by pronucleus injections (Gow et al., 1992; Orian et al., 1994).”
From Recombinant Human Myelin-Associated Glycoprotein Promoter Drives Selective AAV-Mediated Transgene Expression in Oligodendrocytes (2016) · cites this paper as Gow et al., 1992
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“Like the 2,3-cyclic nucleotide 3-phosphodiesterase promoter both Mbp promoters were selected as they had been shown to drive transgenes in the oligodendrocyte lineage in transgenic mouse lines generated by pronucleus injections (Gow et al., 1992; Orian et al., 1994).”
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- Authors
- Gow, A., Friedrich, V. L., & Lazzarini, R. A.
- Affiliations
- Icahn School of Medicine at Mount Sinai (US)
- ORCID
- Gow, A
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1992-11-01
- Volume
- 119
- Issue
- 3
- Pages
- 605-616
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2289674
- OpenAlex
- W2108627571
- Cited by
- 147
- References
- 53
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 2 · 2014: 4 · 2012: 4
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns029056-04)
- National Institute of Neurological Disorders and Stroke (NS29056-02)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Keywords
- myelin basic protein gene, enhancers, cell-specific transcription, promoter, transgenic mice, core promoter
- MeSH terms
- Animals, Base Sequence, beta-Galactosidase, Brain, Cell Nucleus, DNA, Myelin Basic Protein, Enhancer Elements, Genetic, Mice, Transgenic, Molecular Sequence Data, Oligodendroglia, Organ Specificity, Promoter Regions, Genetic, Recombinant Fusion Proteins, Schwann Cells, Spinal Cord, Restriction Mapping, Gene Expression, Mice
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