Calcium Dependence of Exo- and Endocytotic Coupling at a Glutamatergic Synapse
Open accessAbstract
The mechanism coupling exocytosis and endocytosis remains to be elucidated at central synapses. Here, we show that the mechanism linking these two processes is dependent on microdomain-[Ca2+](i) similar to that which triggers exocytosis, as well as the exocytotic protein synaptobrevin/VAMP. Furthermore, block of endocytosis has a limited, retrograde action on exocytosis, delaying recruitment of release-ready vesicles and enhancing short-term depression. This effect sets in so rapidly that it cannot be explained by the nonavailability of recycled vesicles. Rather, we postulate that perturbation of a step linking exocytosis and endocytosis temporarily prevents new vesicles from docking at specialized sites for exocytosis.
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Several previous studies suggest that endocytosis becomes slower when neurons are treated with the high-affinity calcium chelator EGTA, which abolishes asynchronous release.
“Consistent with this notion, several previous studies suggest that endocytosis becomes slower when neurons are treated with the high-affinity calcium chelator EGTA, which abolishes asynchronous release77–81.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [79]
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Balaji, J., & Ryan, T. A. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707574105 · Cited by 279 · see citing works
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Self-citationMembrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis
Ogunmowo, T. H., Jing, H., Raychaudhuri, S., Kusick, G. F., Imoto, Y., Li, S., Itoh, K., Ma, Y., Jafri, H., Dalva, M. B., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-38595-2 · Cited by 51 · see citing works
- Authors
- Hosoi, N., Holt, M., & Sakaba, T.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- Max Planck Society (DE)
- Corresponding author
- Sakaba, Takeshi
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2009-07
- Volume
- 63
- Issue
- 2
- Pages
- 216-229
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2063618791
- Cited by
- 256
- References
- 1
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 7 · 2023: 15 · 2022: 17 · 2021: 7 · 2020: 15 · 2019: 14 · 2018: 16 · 2017: 22 · 2016: 16 · 2015: 20 · 2014: 20 · 2013: 18 · 2012: 22
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1513 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Lirias (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- exocytosis, endocytosis, synaptic vesicle recycling, calcium microdomains, glutamatergic synapses, short-term depression, vesicle docking
- MeSH terms
- Animals, Brain Stem, Calcium, Endocytosis, Exocytosis, Synaptic Transmission, Neurons, Organ Culture Techniques, Synapses, Rats, Wistar, Presynaptic Terminals, Glutamic Acid, Excitatory Postsynaptic Potentials, Calcium Signaling, Dynamins, R-SNARE Proteins, Rats
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