Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses
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Mechanical force is needed to mediate endocytosis. Whether actin, the most abundant force-generating molecule, is essential for endocytosis is highly controversial in mammalian cells, particularly synapses, likely due to the use of actin blockers, the efficiency and specificity of which are often unclear in the studied cell. Here we addressed this issue using a knockout approach combined with measurements of membrane capacitance and fission pore conductance, imaging of vesicular protein endocytosis, and electron microscopy. We found that two actin isoforms, β- and γ-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses. Polymerized actin provides mechanical force to form endocytic pits. Actin also facilitates replenishment of the readily releasable vesicle pool, likely via endocytic clearance of active zones. We conclude that polymerized actin provides mechanical force essential for all kinetically distinguishable forms of endocytosis at synapses.
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Genetic ablation of actin in mammalian neurons strongly perturbs endocytosis.
“Nevertheless, genetic ablation of actin in mammalian neurons strongly perturbs endocytosis;87 like in yeast, active actin polymerization may be important for endocytosis at synapses.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [87]
Like in yeast, active actin polymerization may be important for endocytosis at synapses.
“Nevertheless, genetic ablation of actin in mammalian neurons strongly perturbs endocytosis;87 like in yeast, active actin polymerization may be important for endocytosis at synapses.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [87]
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- Authors
- Wu, X.-S., Lee, S. H., Sheng, J., Zhang, Z., Zhao, W.-D., Wang, D., Jin, Y., Charnay, P., Ervasti, J. M., & Wu, L.-G.
- Affiliations
- National Institute of Neurological Disorders and Stroke (US)
- Centre National de la Recherche Scientifique (FR)
- University of Minnesota (US)
- Corresponding author
- Sheng, Jiansong
- Zhang, Zhen
- Wu, Ling-Gang
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2016-12
- Volume
- 92
- Issue
- 5
- Pages
- 1020-1035
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC5145734
- OpenAlex
- W2549187804
- Cited by
- 109
- References
- 50
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 12 · 2023: 15 · 2022: 15 · 2021: 12 · 2020: 11 · 2019: 8 · 2018: 10 · 2017: 12 · 2016: 1
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (Z99 NS999999)
- National Institute on Deafness and Other Communication Disorders (R01DC005200)
- National Institute of General Medical Sciences (R01GM065358)
- National Institute of Neurological Disorders and Stroke (Z01NS003009)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR049899)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- actin, synaptic endocytosis, beta-actin, gamma-actin, actin polymerization, mechanical forces, hippocampal synapses, membrane capacitance
- MeSH terms
- Actins, Animals, Endocytosis, Hippocampus, Immunohistochemistry, Kinetics, Microscopy, Electron, Neurons, Synapses, Blotting, Western, Presynaptic Terminals, Mice, Knockout, Transport Vesicles, Mice
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