Cited by 168Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

v-SNARE composition distinguishes synaptic vesicle pools

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Hua, Zhaolin, Leal-Ortiz, Sergio, Foss, Sarah M., Waites, Clarissa L., Garner, Craig C., Voglmaier, Susan M., Edwards, Robert H.·2011·Neuron

Abstract

Synaptic vesicles belong to two distinct pools, a recycling pool responsible for the evoked release of neurotransmitter and a resting pool unresponsive to stimulation. The uniform appearance of synaptic vesicles has suggested that differences in location or cytoskeletal association account for these differences in function. We now find that the v-SNARE tetanus toxin-insensitive vesicle-associated membrane protein (VAMP7) differs from other synaptic vesicle proteins in its distribution to the two pools, providing evidence that they differ in molecular composition. We also find that both resting and recycling pools undergo spontaneous release, and when activated by deletion of the longin domain, VAMP7 influences the properties of release. Further, the endocytosis that follows evoked and spontaneous release differs in mechanism, and specific sequences confer targeting to the different vesicle pools. The results suggest that different endocytic mechanisms generate synaptic vesicles with different proteins that can endow the vesicles with distinct properties.

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Crossref · 10.1016/j.neuron.2011.06.010

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  • This Ca2+ elevation specifically increases the fusion of vesicles containing vesicle-associated membrane protein 7 (VAMP7), an alternative SNARE protein that participates in spontaneous vesicle release.

    “This Ca2+ elevation specifically increases the fusion of vesicles containing vesicle-associated membrane protein 7 (VAMP7), an alternative ‘soluble N-ethylmaleimide sensitive factor attachment protein receptor’ (SNARE) protein that participates in spontaneous vesicle release [74].”

    From ApoE, ApoE Receptors, and the Synapse in Alzheimer's Disease (2017) · cites this paper as [74]

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Authors
Hua, Z., Leal-Ortiz, S., Foss, S. M., Waites, C. L., Garner, C. C., Voglmaier, S. M., & Edwards, R. H.
Affiliations
  • University of California, San Francisco (US)
  • Stanford University (US)
Corresponding author
Edwards, Robert H.
Venue
Neuron
Publisher
Elsevier BV
Published
2011-08
Volume
71
Issue
3
Pages
474-487
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2003748392
Cited by
168
References
76
Citations by year
2026: 6 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 8 · 2021: 7 · 2020: 6 · 2019: 9 · 2018: 12 · 2017: 16 · 2016: 11 · 2015: 15 · 2014: 19 · 2013: 22 · 2012: 20
FWCI
9.82
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • American Heart Association
  • National Institute of Mental Health (R01MH050712, R01MH083691, K08MH071626)
  • National Institute on Drug Abuse (P01DA010154)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
synaptic vesicle pools, VAMP7, VAMP, synaptic vesicle recycling, spontaneous neurotransmitter release, longin domain, endocytosis, vesicle composition, vesicle targeting
MeSH terms
Animals, Cells, Cultured, Exocytosis, Mice, Mutant Strains, Neurons, Synaptic Vesicles, Adaptor Protein Complex 3, Adaptor Protein Complex beta Subunits, Vesicular Glutamate Transport Protein 1, SNARE Proteins, R-SNARE Proteins, Mice

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