Stem cell-derived neurons reflect features of protein networks, neuropathology, and cognitive outcome of their aged human donors
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We have generated a controlled and manipulable resource that captures genetic risk for Alzheimer's disease: iPSC lines from 53 individuals coupled with RNA and proteomic profiling of both iPSC-derived neurons and brain tissue of the same individuals. Data collected for each person include genome sequencing, longitudinal cognitive scores, and quantitative neuropathology. The utility of this resource is exemplified here by analyses of neurons derived from these lines, revealing significant associations between specific Aβ and tau species and the levels of plaque and tangle deposition in the brain and, more importantly, with the trajectory of cognitive decline. Proteins and networks are identified that are associated with AD phenotypes in iPSC neurons, and relevant associations are validated in brain. The data presented establish this iPSC collection as a resource for investigating person-specific processes in the brain that can aid in identifying and validating molecular pathways underlying AD.
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We detected several SHIP1 binding partners previously identified in other myeloid cells, such as SH3KBP1, GRB2, SHC1, DOK2, ARAP1, CD2AP.
“We detected several SHIP1 binding partners previously identified in other myeloid cells, such as SH3KBP117, GRB218, SHC118, DOK218,19, ARAP120 and CD2AP21 (Figure 1D).”
From INPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia (2025) · cites this paper as [17]
Citation 21 is attached specifically to CD2AP. The cited paper’s Results discuss protein networks in stem-cell-derived neurons and Alzheimer’s disease, but the passages available from our copy do not address CD2AP as a SHIP1 binding partner in myeloid cells. Searches for CD2AP, SHIP1/INPP5D, and the other named partners found no supporting passage; page numbers were not provided. (LLM, conf=0.76)
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Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890 · see citing works
Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625 · see citing works
Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855 · see citing works
Structuring and extracting knowledge for the support of hypothesis generation in molecular biology
Roos, M., Marshall, M. S., Gibson, A. P., Schuemie, M., Meij, E., Katrenko, S., van Hage, W. V., Krommydas, K., & Adriaans, P. (2009) · BMC Bioinformatics · doi:10.1186/1471-2105-10-s10-s9 · Cited by 11,123
Mootha, V. K., Lindgren, C. M., Eriksson, K.-F., Subramanian, A., Sihag, S., Lehar, J., Puigserver, P., Carlsson, E., Ridderstråle, M., Laurila, E., et al. (2003) · Nature Genetics · doi:10.1038/ng1180 · Cited by 10,197 · see citing works
Near-optimal probabilistic RNA-seq quantification
Bray, N. L., Pimentel, H., Melsted, P., & Pachter, L. (2016) · Nature Biotechnology · doi:10.1038/nbt.3519 · Cited by 9,723 · see citing works
Adjusting batch effects in microarray expression data using empirical Bayes methods
Johnson, W. E., Li, C., & Rabinovic, A. (2007) · Biostatistics · doi:10.1093/biostatistics/kxj037 · Cited by 8,658 · see citing works
The amyloid hypothesis of Alzheimer's disease at 25 years
Selkoe, D. J., & Hardy, J. (2016) · EMBO Molecular Medicine · doi:10.15252/emmm.201606210 · Cited by 6,164 · see citing works
Self-citationTerry, R. D., Masliah, E., Salmon, D. P., Butters, N., DeTeresa, R., Hill, R., Hansen, L. A., & Katzman, R. (1991) · Annals of Neurology · doi:10.1002/ana.410300410 · Cited by 4,397 · see citing works
Meta-Analysis of 74,046 Individuals Identifies 11 New Susceptibility Loci for Alzheimer’s Disease
(EADI), E. A. D. I., Lambert, J.-C., (GERAD), G. A. E. R. I. A. D., Alzheimer's Disease Genetic Consortium (ADGC), (CHARGE), C. F. H. A. A. R. I. G. E., Ibrahim-Verbaas, C. A., Harold, D., Naj, A. C., Sims, R., Bellenguez, C., et al. (2013) · Nature Genetics · doi:10.1038/ng.2802 · Cited by 4,336 · see citing works
Self-citationAlzheimer Disease Genetics Consortium (ADGC), Kunkle, B. W., The European Alzheimer’s Disease Initiative (EADI), Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium (CHARGE), Genetic and Environmental Risk in AD/Defining Genetic, P. A. E. R. F. A. D. C. (., Grenier-Boley, B., Sims, R., Bis, J. C., Damotte, V., Naj, A. C., et al. (2019) · Nature Genetics · doi:10.1038/s41588-019-0358-2 · Cited by 2,946 · see citing works
Self-citationScheuner, D., Eckman, C., Jensen, M., Song, X., Citron, M., Suzuki, N., Bird, T. D., Hardy, J., Hutton, M., Kukull, W., et al. (1996) · Nature Medicine · doi:10.1038/nm0896-864 · Cited by 2,599 · see citing works
Self-citationJansen, I. E., Savage, J. E., Watanabe, K., Bryois, J., Williams, D. M., Steinberg, S., Sealock, J., Karlsson, I. K., Hägg, S., Athanasiu, L., et al. (2019) · Nature Genetics · doi:10.1038/s41588-018-0311-9 · Cited by 2,277 · see citing works
Integrated Systems Approach Identifies Genetic Nodes and Networks in Late-Onset Alzheimer’s Disease
Zhang, B., Gaiteri, C., Bodea, L.-G., Wang, Z., McElwee, J., Podtelezhnikov, A. A., Zhang, C., Xie, T., Tran, L., Dobrin, R., et al. (2013) · Cell · doi:10.1016/j.cell.2013.03.030 · Cited by 1,657 · see citing works
Self-citationRapid single-step induction of functional neurons from human pluripotent stem cells
Zhang, Y., Pak, C., Han, Y., Ahlenius, H., Zhang, Z., Chanda, S., Marro, S., Patzke, C., Acuna, C., Covy, J., et al. (2013) · Neuron · doi:10.1016/j.neuron.2013.05.029 · Cited by 1,644 · see citing works
Formation of Neurofibrillary Tangles in P301L Tau Transgenic Mice Induced by Aβ42 Fibrils
Götz, J., Chen, F., van Dorpe, J., & Nitsch, R. M. (2001) · Science · doi:10.1126/science.1062097 · Cited by 1,526 · see citing works
Power and predictive accuracy of polygenic risk scores
Dudbridge, F. (2013) · PLoS Genetics · doi:10.1371/journal.pgen.1003348 · Cited by 1,509 · see citing works
Differential analysis of RNA-seq incorporating quantification uncertaintyPreprint
Pimentel, H., Bray, N. L., Puente, S., Melsted, P., & Pachter, L. (2016) · Nature Methods · doi:10.1101/058164 · Cited by 1,401 · see citing works
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation
Kang, H. M., Subramaniam, M., Targ, S., Nguyen, M., Maliskova, L., McCarthy, E., Wan, E., Wong, S., Byrnes, L., Lanata, C. M., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4042 · Cited by 1,339 · see citing works
Religious Orders Study and Rush Memory and Aging Project
Bennett, D. A., Buchman, A. S., Boyle, P. A., Barnes, L. L., Wilson, R. S., & Schneider, J. A. (2018) · Journal of Alzheimer's Disease · doi:10.3233/jad-179939 · Cited by 1,260 · see citing works
Self-citationProbing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells
Israel, M. A., Yuan, S. H., Bardy, C., Reyna, S. M., Mu, Y., Herrera, C., Hefferan, M. P., Van Gorp, S., Nazor, K. L., Boscolo, F. S., et al. (2012) · Nature · doi:10.1038/nature10821 · Cited by 1,253 · see citing works
Induction of human neuronal cells by defined transcription factors
Pang, Z. P., Yang, N., Vierbuchen, T., Ostermeier, A., Fuentes, D., Yang, T. Q., Citri, A., Sebastiano, V., Marro, S. G., Südhof, T. C., et al. (2011) · Nature · doi:10.1038/nature10202 · Cited by 1,249 · see citing works
iPSC-derived human microglia-like cells to study neurological diseases
Abud, E. M., Ramirez, R. N., Martinez, E. S., Healy, L. M., Nguyen, C. H. H., Newman, S. A., Yeromin, A. V., Scarfone, V. M., Marsh, S. E., Fimbres, C., et al. (2017) · Neuron · doi:10.1016/j.neuron.2017.03.042 · Cited by 1,154 · see citing works
Johnson, E. C. B., Dammer, E. B., Duong, D. M., Ping, L., Zhou, M., Yin, L., Higginbotham, L. A., Guajardo, A., White, B., Troncoso, J. C., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0815-6 · Cited by 1,001 · see citing works
Self-citationJin, M., Shepardson, N., Yang, T., Chen, G., Walsh, D., & Selkoe, D. J. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1017033108 · Cited by 876 · see citing works
Self-citationOverview and Findings from the Rush Memory and Aging Project
Bennett, D. A., Schneider, J. A., Buchman, A. S., Barnes, L. L., Boyle, P. A., & Wilson, R. S. (2012) · Current Alzheimer Research · doi:10.2174/156720512801322663 · Cited by 837 · see citing works
Self-citationThe Genetics of Alzheimer Disease: Back to the Future
Bertram, L., Lill, C. M., & Tanzi, R. E. (2010) · Neuron · doi:10.1016/j.neuron.2010.10.013 · Cited by 834 · see citing works
Kondo, T., Asai, M., Tsukita, K., Kutoku, Y., Ohsawa, Y., Sunada, Y., Imamura, K., Egawa, N., Yahata, N., Okita, K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.01.009 · Cited by 741 · see citing works
Overview and Findings from the Religious Orders Study
Bennett, D. A., Schneider, J. A., Arvanitakis, Z., & Wilson, R. S. (2012) · Current Alzheimer Research · doi:10.2174/156720512801322573 · Cited by 647 · see citing works
Self-citationBennett, D. A., Schneider, J. A., Wilson, R. S., Bienias, J. L., & Arnold, S. E. (2004) · Archives of Neurology · doi:10.1001/archneur.61.3.378 · Cited by 526 · see citing works
Self-citationMostafavi, S., Gaiteri, C., Sullivan, S. E., White, C. C., Tasaki, S., Xu, J., Taga, M., Klein, H.-U., Patrick, E., Komashko, V., et al. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0154-9 · Cited by 509 · see citing works
Self-citationSeyfried, N. T., Dammer, E. B., Swarup, V., Nandakumar, D., Duong, D. M., Yin, L., Deng, Q., Nguyen, T., Hales, C. M., Wingo, T., et al. (2017) · Cell Systems · doi:10.1016/j.cels.2016.11.006 · Cited by 477 · see citing works
Self-citationAn xQTL map integrates the genetic architecture of the human brain's transcriptome and epigenome
Ng, B., White, C. C., Klein, H.-U., Sieberts, S. K., McCabe, C., Patrick, E., Xu, J., Yu, L., Gaiteri, C., Bennett, D. A., et al. (2017) · Nature Neuroscience · doi:10.1038/nn.4632 · Cited by 452 · see citing works
Self-citationMcQuade, A., Coburn, M., Tu, C. H., Hasselmann, J., Davtyan, H., & Blurton-Jones, M. (2018) · Molecular Neurodegeneration · doi:10.1186/s13024-018-0297-x · Cited by 449 · see citing works
Tau molecular diversity contributes to clinical heterogeneity in Alzheimer’s disease
Dujardin, S., Commins, C., Lathuiliere, A., Beerepoot, P., Fernandes, A. R., Kamath, T. V., De Los Santos, M. B., Klickstein, N., Corjuc, D. L., Corjuc, B. T., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0938-9 · Cited by 438 · see citing works
Self-citationWang, C., Najm, R., Xu, Q., Jeong, D.-E., Walker, D., Balestra, M. E., Yoon, S. Y., Yuan, H., Li, G., Miller, Z. A., et al. (2018) · Nature Medicine · doi:10.1038/s41591-018-0004-z · Cited by 428 · see citing works
Presenilin clinical mutations can affect γ‐secretase activity by different mechanisms
Bentahir, M., Nyabi, O., Verhamme, J., Tolia, A., Horré, K., Wiltfang, J., Esselmann, H., & Strooper, B. D. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03578.x · Cited by 412 · see citing works
Muratore, C. R., Rice, H. C., Srikanth, P., Callahan, D. G., Shin, T., Benjamin, L. N. P., Walsh, D. M., Selkoe, D. J., & Young-Pearse, T. L. (2014) · Human Molecular Genetics · doi:10.1093/hmg/ddu064 · Cited by 379 · see citing works
Self-citationCeramide stimulates a cytosolic protein phosphatase.
Dobrowsky, R., & Hannun, Y. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42727-5 · Cited by 376 · see citing works
Zhao, J., Fu, Y., Yamazaki, Y., Ren, Y., Davis, M. D., Liu, C.-C., Lu, W., Wang, X., Chen, K., Cherukuri, Y., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19264-0 · Cited by 374 · see citing works
Wang, C., Xiong, M., Gratuze, M., Bao, X., Shi, Y., Andhey, P. S., Manis, M., Schroeder, C., Yin, Z., Madore, C., et al. (2021) · Neuron · doi:10.1016/j.neuron.2021.03.024 · Cited by 334 · see citing works
Kwart, D., Gregg, A., Scheckel, C., Murphy, E. A., Paquet, D., Duffield, M. D., Fak, J. J., Olsen, O., Darnell, R. B., & Tessier‐Lavigne, M. (2019) · Neuron · doi:10.1016/j.neuron.2019.07.010 · Cited by 317 · see citing works
A Human Stem Cell Model of Early Alzheimer’s Disease Pathology in Down Syndrome
Shi, Y., Kirwan, P., Smith, J., MacLean, G., Orkin, S. H., & Livesey, F. J. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003771 · Cited by 310 · see citing works
Long Chain Ceramides Activate Protein Phosphatase-1 and Protein Phosphatase-2A
Chalfant, C. E., Kishikawa, K., Mumby, M. C., Kamibayashi, C., Bielawska, A. E., & Hannun, Y. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.29.20313 · Cited by 300 · see citing works
Role of ceramide-activated protein phosphatase in ceramide-mediated signal transduction.
Wolff, R. A., Dobrowsky, R., Bielawska, A., Obeid, L., & Hannun, Y. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32212-3 · Cited by 277 · see citing works
Paull, D., Sevilla, A., Zhou, H., Hahn, A. K., Kim, H., Napolitano, C., Tsankov, A., Shang, L., Krumholz, K., Jagadeesan, P., et al. (2015) · Nature Methods · doi:10.1038/nmeth.3507 · Cited by 263 · see citing works
Self-citationNehme, R., Zuccaro, E., Ghosh, S. D., Li, C., Sherwood, J. L., Pietiläinen, O. P. H., Barrett, L. E., Limone, F., Worringer, K. A., Kommineni, S., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.066 · Cited by 242 · see citing works
Endo-lysosomal dysregulations and late-onset Alzheimer’s disease: impact of genetic risk factors
Van Acker, Z. P., Bretou, M., & Annaert, W. (2019) · Molecular Neurodegeneration · doi:10.1186/s13024-019-0323-7 · Cited by 222 · see citing works
Rapid and efficient induction of functional astrocytes from human pluripotent stem cells
Canals, I., Ginisty, A., Quist, E., Timmerman, R., Fritze, J., Miskinyte, G., Monni, E., Hansen, M. G., Hidalgo, I., Bryder, D., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0103-2 · Cited by 212 · see citing works
Hsieh, Y.-C., Guo, C., Yalamanchili, H. K., Abreha, M., Al-Ouran, R., Li, Y., Dammer, E. B., Lah, J. J., Levey, A. I., Bennett, D. A., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.08.104 · Cited by 196 · see citing works
Self-citationMemory complaints are related to Alzheimer disease pathology in older persons
Barnes, L. L., Schneider, J. A., Boyle, P. A., Bienias, J. L., & Bennett, D. A. (2006) · Neurology · doi:10.1212/01.wnl.0000242734.16663.09 · Cited by 192 · see citing works
Self-citationAPP Metabolism Regulates Tau Proteostasis in Human Cerebral Cortex Neurons.
Moore, S., Evans, L. D. B., Andersson, T., Portelius, E., Smith, J., Dias, T. B., Saurat, N., McGlade, A., Kirwan, P., Blennow, K., et al. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.03.068 · Cited by 179 · see citing works
Sproul, A. A., Jacob, S., Prè, D., Kim, S. H., Nestor, M. W., Navarro-Sobrino, M., Santa‐María, I., Zimmer, M., Aubry, S., Steele, J., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0084547 · Cited by 179 · see citing works
Self-citationRapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells
Ho, S., Hartley, B. J., Julia, T. C. W., Beaumont, M. A., Stafford, K., Slesinger, P. A., & Brennand, K. (2015) · Methods · doi:10.1016/j.ymeth.2015.11.019 · Cited by 173 · see citing works
Felsky, D., Roostaei, T., Nho, K., Risacher, S. L., Bradshaw, E. M., Petyuk, V., Schneider, J. A., Saykin, A., Bennett, D. A., & De Jager, P. L. (2019) · Nature Communications · doi:10.1038/s41467-018-08279-3 · Cited by 169 · see citing works
Self-citationSynaptic proteins predict cognitive decline in Alzheimer's disease and Lewy body dementia
Bereczki, E., Francis, P. T., Howlett, D., Pereira, J. B., Höglund, K., Bogstedt, A., Cedazo‐Minguez, A., Baek, J., Hortobágyi, T., Attems, J., et al. (2016) · Alzheimer's & Dementia · doi:10.1016/j.jalz.2016.04.005 · Cited by 167 · see citing works
Hung, C. O. Y., & Livesey, F. J. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.095 · Cited by 159 · see citing works
Mild cognitive impairment in different functional domains and incident Alzheimer’s disease
Aggarwal, N., Wilson, R., Beck, T., Bienias, J., & Bennett, D. (2005) · Journal of Neurology Neurosurgery & Psychiatry · doi:10.1136/jnnp.2004.053561 · Cited by 151 · see citing works
Self-citationEducation modifies the association of amyloid but not tangles with cognitive function
Bennett, D. A., Schneider, J. A., Wilson, R. S., Bienias, J. L., & Arnold, S. E. (2005) · Neurology · doi:10.1212/01.wnl.0000176286.17192.69 · Cited by 121 · see citing works
Self-citationKnobloch, M., Farinelli, M., Konietzko, U., Nitsch, R. M., & Mansuy, I. M. (2007) · Journal of Neuroscience · doi:10.1523/jneurosci.0395-07.2007 · Cited by 117 · see citing works
Terminal Cognitive Decline: Accelerated Loss of Cognition in the Last Years of Life
Wilson, R. S., Beck, T. L., Bienias, J. L., & Bennett, D. A. (2007) · Psychosomatic Medicine · doi:10.1097/psy.0b013e31803130ae · Cited by 117 · see citing works
Self-citationMuratore, C. R., Zhou, C., Liao, M., Fernandez, M. A., Taylor, W. M., Lagomarsino, V. N., Pearse, R., Rice, H. C., Negri, J. M., He, A., et al. (2017) · Stem Cell Reports · doi:10.1016/j.jalz.2017.07.071 · Cited by 91
Self-citationIntegrating Gene and Protein Expression Reveals Perturbed Functional Networks in Alzheimer’s Disease
Canchi, S., Raao, B., Masliah, D., Rosenthal, S. B., Šášik, R., Fisch, K. M., Jager, P. L. D., Bennett, D. A., & Rissman, R. A. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.06.073 · Cited by 81 · see citing works
Self-citationNegri, J. M., Menon, V., & Young‐Pearse, T. L. (2020) · eNeuro · doi:10.1523/eneuro.0080-19.2019 · Cited by 52 · see citing works
Self-citationPremorbid proneness to distress and episodic memory impairment in Alzheimer’s disease
Wilson, R., Fleischman, D., Myers, R. A., Bennett, D. A., Bienias, J., Gilley, D., & Evans, D. A. (2004) · Journal of Neurology Neurosurgery & Psychiatry · doi:10.1136/jnnp.2003.015040 · Cited by 45
Self-citationPolygenic analysis of inflammatory disease variants and effects on microglia in the aging brain
Felsky, D., Patrick, E., Schneider, J. A., Mostafavi, S., Gaiteri, C., Patsopoulos, N. A., Bennett, D. A., & Jager, P. L. D. (2018) · Molecular Neurodegeneration · doi:10.1186/s13024-018-0272-6 · Cited by 44 · see citing works
Self-citationRapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood
Zhou, H.-Y., Martinez, H., Sun, B., Li, A.-Q., Zimmer, M., Katsanis, N., Davis, E., Kurtzberg, J., Lipnick, S., Noggle, S., et al. (2015) · Stem Cell Reviews and Reports · doi:10.1007/s12015-015-9586-8 · Cited by 41 · see citing works
Self-citationLiu, L., Lauro, B. M., Wolfe, M. S., & Selkoe, D. J. (2021) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2021.100393 · Cited by 40 · see citing works
Self-citationBarnes, L. L., Nelson, J. K., & Reuter‐Lorenz, P. A. (2001) · Progress in brain research · doi:10.1016/s0079-6123(01)34031-1 · Cited by 30 · see citing works
Tasaki, S., Gaiteri, C., Mostafavi, S., Yu, L., Wang, Y., Jager, P. L. D., & Bennett, D. A. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00294 · Cited by 26 · see citing works
Self-citationConvergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression
Srikanth, P., Lagomarsino, V. N., Pearse, R. V., Liao, M., Ghosh, S. D., Nehme, R., Seyfried, N. T., Eggan, K. C., & Young‐Pearse, T. L. (2018) · Translational Psychiatry · doi:10.1038/s41398-018-0281-9 · Cited by 25 · see citing works
Self-citation
Flagged first, then by citations
2 of the 73 references couldn’t be matched to a known paper.
Research works printed but not linked: 3
- GARCIA-ESPARCIA P, SIDERIS-LAMPRETSAS G, HERNANDEZ-ORTEGA K, GRAU-RIVERA O, SKLAVIADIS T, GELPI E & FERRER I 2017. Altered mechanisms of protein synthesis in frontal cortex in Alzheimer disease and a mouse model. Am J Neurodegener Dis, 6, 15– 25. 28695061 PMC5498849
- ODDO S, BILLINGS L, KESSLAK JP, CRIBBS DH & LAFERLA FM 2004. Abeta immunotherapy leads to clearance of early, but not late, hyperphosphorylated tau aggregates via the proteasome. Neuron, 43, 321– 32. 15294141
- PAULL D, SEVILLA A, ZHOU H, HAHN AK, KIM H, NAPOLITANO C, TSANKOV A, SHANG L, KRUMHOLZ K, JAGADEESAN P, WOODARD CM, SUN B, VILBOUX T, ZIMMER M, FORERO E, MOROZIEWICZ DN, MARTINEZ H, MALICDAN MC, WEISS KA, VENSAND LB, DUSENBERRY CR, POLUS H, SY KT, KAHLER DJ, GAHL WA, SOLOMON SL, CHANG S, MEISSNER A, EGGAN K & NOGGLE SA Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat Methods. PMC13012702 26237226
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Fujita, M., Gao, Z., Zeng, L., Aubin, C., White, C. C., Ng, B., Green, G. S., Rozenblatt–Rosen, O., Phillips, D., Amir-Zilberstein, L., et al. (2024) · Nature Genetics · doi:10.1038/s41588-024-01685-y · Cited by 189 · see citing works
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Clark, L. E., Clark, S. A., Lin, C., Liu, J., Coscia, A., Smith, K. N., Yang, P., Neel, D. V., Lee, H., Brusic, V., et al. (2021) · Nature · doi:10.1038/s41586-021-04326-0 · Cited by 130 · see citing works
Self-citationGasdermin-E mediates mitochondrial damage in axons and neurodegenerationPreprint
Neel, D. V., Basu, H., Gunner, G., Bergstresser, M., Giadone, R., Chung, H., Miao, R., Chou, V., Brody, E. M., Jiang, X., et al. (2022) · bioRxiv · doi:10.1101/2022.11.01.513927 · Cited by 123 · see citing works
Self-citationNeuronal activity-dependent mechanisms of small cell lung cancer pathogenesis
Savchuk, S., Gentry, K. M., Wang, W., Carleton, E., de Biagi, C. A. O., Luthria, K. D., Yalçın, B., Ni, L., Farnsworth, H. C., Davis, R. A., et al. (2025) · Nature · doi:10.1038/s41586-025-09492-z · Cited by 131 · see citing works
Moving beyond amyloid and tau to capture the biological heterogeneity of Alzheimer’s disease
Young‐Pearse, T. L., Lee, H., Hsieh, Y., Chou, V., & Selkoe, D. J. (2023) · Trends in Neurosciences · doi:10.1016/j.tins.2023.03.005 · Cited by 99 · see citing works
Self-citationINPP5D regulates inflammasome activation in human microglia
Chou, V., Pearse, R. V., Aylward, A. J., Ashour, N., Taga, M., Terzioglu, G., Fujita, M., Fancher, S. B., Sigalov, A., Benoit, C. R., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-42819-w · Cited by 79 · see citing works
Self-citationRajabli, F., Beecham, G., Hendrie, H., Baiyewu, O., Ogunniyi, A., Gao, S.-J., Kushch, N. A., Lipkin-Vasquez, M., Hamilton-Nelson, K., Young, J. I., et al. (2022) · PLoS Genetics · doi:10.1371/journal.pgen.1009977 · Cited by 63 · see citing works
Ji, Y., Chen, X., Wang, Z., Meek, C. J., McLean, J. L., Yang, Y., Yuan, C., Rochet, J., Liu, F., & Xu, R. (2025) · Molecular Psychiatry · doi:10.1038/s41380-025-03041-w · Cited by 65 · see citing works
Saurat, N. G., Minotti, A. P., Rahman, M. T., Sikder, T., Zhang, C., Cornacchia, D., Jungverdorben, J., Ciceri, G., Betel, D., & Studer, L. (2024) · Cell stem cell · doi:10.1016/j.stem.2024.06.001 · Cited by 58 · see citing works
Webber, E. K., Fivaz, M., Stutzmann, G. E., & Griffioen, G. (2023) · Alzheimer's & Dementia · doi:10.1002/alz.13065 · Cited by 50 · see citing works
Lee, H., Aylward, A. J., Pearse, R. V., Lish, A. M., Hsieh, Y., Augur, Z. M., Benoit, C. R., Chou, V., Knupp, A., Pan, C., et al. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112994 · Cited by 53 · see citing works
Self-citationThe future of stem cell therapies of Alzheimer’s disease
Wang, Z., Wang, Z.-T., Sun, Y., Tan, L., & Yu, J. (2022) · Ageing Research Reviews · doi:10.1016/j.arr.2022.101655 · Cited by 62 · see citing works
Lish, A. M., Grogan, E. F. L., Benoit, C. R., Pearse, R. V., Heuer, S. E., Luquez, T., Orme, G. A., Galle, P. C., Milinkeviciute, G., Green, K. N., et al. (2025) · Neuron · doi:10.1016/j.neuron.2025.03.034 · Cited by 53 · see citing works
Self-citationNeurodegeneration cell per cell
Balusu, S., Praschberger, R., Lauwers, E., Strooper, B. D., & Verstreken, P. (2023) · Neuron · doi:10.1016/j.neuron.2023.01.016 · Cited by 51 · see citing works
“Studies using iPSC models derived from sporadic AD cases 257 or from familial AD cases 258 concluded that neurons derived from iPSC, still capture AD-relevant signatures.”
3D hydrogel models of the neurovascular unit to investigate blood–brain barrier dysfunction
Potjewyd, G., Kellett, K. A. B., & Hooper, N. M. (2021) · Neuronal Signaling · doi:10.1042/ns20210027 · Cited by 54 · see citing works
Labib, D., Wang, Z., Prakash, P. S., Zimmer, M., Smith, M. D., Frazel, P. W., Barbar, L., Sapar, M. L., Calabresi, P. A., Peng, J., et al. (2022) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2022.870085 · Cited by 42 · see citing works
“…Recasens et al., 2018 HLA-E–Major Histocompatibility Complex I, E (MHC Class I Antigen E) Coupel et al., 2007; Rölle et al., 2018 Morandi et al., 2013; Lau et al., 2020; Wu et al., 2020 PD-L1– Programmed Cell Death 1 Ligand 1 (CD274; B7H1) Freeman et al., 2000; Cha et al., 2019; Doroshow et al.,…”
BackgroundLiu, L., Lauro, B. M., He, A., Lee, H., Bhattarai, S., Wolfe, M. S., Bennett, D. A., Karch, C. M. M., Young‐Pearse, T. L., Dominantly Inherited Alzheimer Network (DIAN), et al. (2022) · Alzheimer s & Dementia · doi:10.1002/alz.12646 · Cited by 45 · see citing works
“41 We observed the expected dose-dependent increase in short Aβs (37, 38) and decrease in long Aβs (40, 42) (Figure 2D, E).”
BackgroundSelf-citationCRISPR-based functional genomics screening in human-pluripotent-stem-cell-derived cell types
Li, K., Ouyang, M., Zhan, J., & Tian, R. (2023) · Cell Genomics · doi:10.1016/j.xgen.2023.100300 · Cited by 42 · see citing works
“For example, iPSC-derived neurons from patients with familial or sporadic Alzheimer’s disease (AD) exhibit pathological hallmarks of AD such as enlarged early endosomes, increased amyloid-b, and phosphorylated tau.(6,7) iPSC-derived cardiacmyocytes from patients carrying pathogenic mutations of long-QT syndrome, a car…”
BackgroundProtecting Human and Animal Health: The Road from Animal Models to New Approach Methods
Kaplan, B. L. F., Hoberman, A. M., Slikker, W., Smith, M. A., Corsini, E., Knudsen, T. B., Marty, S., Sobrian, S. K., Fitzpatrick, S. C., Ratner, M. H., et al. (2024) · Pharmacological Reviews · doi:10.1124/pharmrev.123.000967 · Cited by 49 · see citing works
“Human-derived induced pluripotent stem cells are used to create brain organoid models of AD (Lagomarsino et al., 2021).”
Krichevsky, A. M., Nguyen, L. D., Wei, Z.-Y., Silva, M., Barberán-Soler, S., Rabinovsky, R., Muratore, C. R., Stricker, J. M. S., Hortman, C., Young-Pearse, T. L., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-2617979/v1 · Cited by 39
Self-citationNovel human iPSC models of neuroinflammation in neurodegenerative disease and regenerative medicine
Summers, R. A., Fagiani, F., Rowitch, D. H., Absinta, M., & Reich, D. S. (2024) · Trends in Immunology · doi:10.1016/j.it.2024.08.004 · Cited by 39 · see citing works
Patient iPSC models reveal glia-intrinsic phenotypes in multiple sclerosis
Clayton, B. L. L., Barbar, L., Sapar, M., Kalpana, K., Rao, C., Migliori, B., Rusielewicz, T., Paull, D., Brenner, K., Moroziewicz, D., et al. (2024) · Cell Stem Cell · doi:10.1016/j.stem.2024.08.002 · Cited by 38 · see citing works
Self-citationRizzo, S. J. S., Homanics, G. E., Schaeffer, D. J., Schaeffer, L. K. H., Park, J. E., Oluoch, J., Zhang, T., Haber, A., Seyfried, N. T., Paten, B., et al. (2023) · Alzheimer s & Dementia Translational Research & Clinical Interventions · doi:10.1002/trc2.12417 · Cited by 35 · see citing works
Self-citationBrain organoids are new tool for drug screening of neurological diseases
Zhou, J.-Q., Zeng, L., Li, C.-T., He, D.-H., Zhao, H.-D., Xu, Y., Jin, Z.-T., & Gao, C. (2023) · Neural Regeneration Research · doi:10.4103/1673-5374.367983 · Cited by 47 · see citing works
“Furthermore, technological combination between iPSC reprogramming and the generation of brain organoids has resulted in a new disease model for neurological or psychiatric diseases (Lancaster et al., 2013; Clevers, 2016; Di Lullo and Kriegstein, 2017; Qian et al., 2019; Cherskov and Sestan, 2021).”
BackgroundMethodStrategies for modeling aging and age-related diseases
Jothi, D., & Kulka, L. A. M. (2024) · npj Aging · doi:10.1038/s41514-024-00161-5 · Cited by 36 · see citing works
Lish, A. M., Ashour, N., Pearse, R. V., Galle, P. C., Orme, G. A., Heuer, S. E., Benoit, C. R., Alexander, K. D., Grogan, E. F. L., Terzioğlu, G., et al. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.115777 · Cited by 29 · see citing works
“35,82 iPSCs were plated at a density of 95k cells/cm 2 on plates coated with growth factor reduced Matrigel one day prior to virus transduction (Corning #354230).”
InfluentialSelf-citationNeuronal-Activity Dependent Mechanisms of Small Cell Lung Cancer ProgressionPreprint
Savchuk, S., Gentry, K., Wang, W.-G., Carleton, E., Yalçın, B., Liu, Y., Pavarino, E. C., LaBelle, J., Toland, A. M. S., Woo, P. J., et al. (2023) · bioRxiv · doi:10.1101/2023.01.19.524430 · Cited by 30 · see citing works
Conserved gene signatures shared among MAPT mutations reveal defects in calcium signaling
Minaya, M., Mahali, S. K., Iyer, A. K., Eteleeb, A. M., Martinez, R. J., Huang, G., Budde, J., Temple, S., Nana, A. L., Seeley, W. W., et al. (2023) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1051494 · Cited by 26 · see citing works
“Human cellular models of the brain derived from induced pluripotent stem cells (iPSC) have become an important tool for studying molecular and cellular markers that may initiate disease (Livesey, 2014; Iovino et al., 2015; Silva et al., 2016; Guo et al., 2017; Wray, 2017; Gonzalez et al., 2018; Jiang et al., 2018; Kar…”
Wu, C.-I., Vinton, E. A., Pearse, R. V., Heo, K., Aylward, A. J., Hsieh, Y., Bi, Y., Adeleye, S., Fancher, S. B., Duong, D. M., et al. (2022) · Molecular Psychiatry · doi:10.1038/s41380-022-01454-5 · Cited by 33 · see citing works
“Neurons derived using this protocol have been extensively characterized using bulk and single cell RNAseq, immunostaining, and proteomics and these studies revealed that this protocol generates a homogeneous population of excitatory glutamatergic neurons that are most similar to neurons in layers 2/3 of the cerebral c…”
BackgroundMethodResultInfluentialSelf-citationSheta, R., Teixeira, M., Idi, W., Pierre, M., Jacquet, A. D. R., Émond, V., Zorca, C. E., Vanderperre, B., Durcan, T. M., Fon, E. A., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-22158-4 · Cited by 35 · see citing works
Schultz, S. A., Liu, L., Schultz, A. P., Fitzpatrick, C. D., Levin, R., Bellier, J., Shirzadi, Z., Joseph‐Mathurin, N., Chen, C. D., Benzinger, T. L. S., et al. (2024) · The Lancet Neurology · doi:10.1016/s1474-4422(24)00236-9 · Cited by 34 · see citing works
Self-citationvan der Linden, R. J., Gerritsen, J. S., Liao, M., Widomska, J., Pearse, R. V., White, F. M., Franke, B., Young‐Pearse, T. L., & Poelmans, G. (2022) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2022.102316 · Cited by 40 · see citing works
Self-citationNeuronal constitutive endolysosomal perforations enable α-synuclein aggregation by internalized PFFs
Sanyal, A., Scanavachi, G., Somerville, E., Saminathan, A., Nair, A., Correia, R. F. B. D. C., Aylan, B., Sitarska, E., Oikonomou, A., Hatzakis, N., et al. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202401136 · Cited by 26 · see citing works
“BR33 iPSCs, as previously described (Paull et al., 2015; Lagomarsino et al., 2021), were kindly provided by Dr. Tracy Young-Pearse (Brigham and Women ’ s Hospital, Boston, MA, USA).”
Uncovering plaque-glia niches in human Alzheimer’s disease brains using spatial transcriptomics
Avey, D. R., Ng, B., Vialle, R. A., Kearns, N. A., Lopes, K. D. P., Iatrou, A., Tissera, S. L. D., Vyas, H., Saunders, D. M., Flood, D. J., et al. (2025) · Molecular Neurodegeneration Advances · doi:10.1186/s44477-025-00002-z · Cited by 26 · see citing works
“Two iPSC lines (AICS-0090–391 purchased from the Coriell Institute and BR28 [34] obtained from Dr. Tracy Young-Pearse) were used for the culture experiments. iPSCs were maintained on Matrigel-coated dishes in mTeSR Plus (StemCell Technologies). iPSCs were passaged every 4 days with ReLeSR (StemCell…”
Self-citationLomoio, S., Pandey, R. S., Rouleau, N., Menicacci, B., Kim, W., Cantley, W. L., Haydon, P. G., Bennett, D. A., Young-Pearse, T. L., Carter, G. W., et al. (2023) · Molecular Psychiatry · doi:10.1038/s41380-023-02147-3 · Cited by 25 · see citing works
Self-citationUncovering Plaque-Glia Niches in Human Alzheimer’s Disease Brains Using Spatial Transcriptomics
Avey, D. R., Ng, B., Vialle, R. A., Kearns, N. A., de Paiva Lopes, K., Iatrou, A., De Tissera, S., Vyas, H., Saunders, D. M., Flood, D. J., et al. (2024) · doi:10.1101/2024.09.05.611566 · Cited by 24
“P -values were adjusted for multiple comparisons using BH-FDR correction. iPSC cultures and differentiation Two iPSC lines (AICS-0090-391 purchased from the Coriell Institute and BR28 63 obtained from Dr. Tracy Young-Pearse) were used for the culture experiments. iPSCs were maintained on Matrigel-824 coated dishes in…”
InfluentialSelf-citationFrazel, P. W., Labib, D., Fisher, T., Brosh, R., Pirjanian, N., Marchildon, A. E., Boeke, J. D., Fossati, V., & Liddelow, S. A. (2023) · Nature Neuroscience · doi:10.1038/s41593-023-01424-2 · Cited by 22 · see citing works
“For our multiline experiment, we used nine iPSC lines from an iPSC collection recently generated at NYSCF to enable large-scale studies on Alzheimer’s disease 66 .”
MethodFan, K., Francis, L., Aslam, M. M., Bedison, M. A., Lawrence, E., Acharya, V., Snitz, B. E., Ganguli, M., DeKosky, S. T., Lopez, O. L., et al. (2023) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2022.11.007 · Cited by 21 · see citing works
Marei, H. E. S., Khan, M. U. A., & Hasan, A. (2023) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-023-00504-2 · Cited by 26 · see citing works
“This has demonstrated the value of using iPSCs to clarify the fundamental pathophysiology of AD in humans [53].”
BackgroundThe Breakthroughs and Caveats of Using Human Pluripotent Stem Cells in Modeling Alzheimer’s Disease
Bendtsen, K. M., & Hall, V. J. (2023) · Cells · doi:10.3390/cells12030420 · Cited by 27 · see citing works
“The study also showed that a reduction of protein phosphatase 1 (PP1) led to an increase in expression of major pTau in the neurons [59].”
InfluentialMustaly-Kalimi, S., Gallegos, W., Steinbrenner, D., Gupta, S. N., Houcek, A., Bennett, D. A., Marr, R. A., Peterson, D. A., Sekler, I., & Stutzmann, G. E. (2025) · Acta Neuropathologica Communications · doi:10.1186/s40478-025-02023-x · Cited by 22 · see citing works
“…used 4 ROSMAP iPSC lines derived from 2 persons with AD (R107: 86-year-old female and R126: > 89-year-old male), and 2 without dementia (R46: >89-year-old female and R38: >89-year-old male) [3, 57], and generated mature electrically excitable neurons that make synaptic contracts (Supplemental Fig.”
Self-citationSit, T. P. H., Feord, R. C., Dunn, A. W., Chabros, J., Oluigbo, D., Smith, H., Burn, L. A., Chang, E., Boschi, A., Yin, Y., et al. (2024) · Cell Reports Methods · doi:10.1016/j.crmeth.2024.100901 · Cited by 22 · see citing works
Person‐specific differences in ubiquitin‐proteasome mediated proteostasis in human neurons
Hsieh, Y.-C., Augur, Z. M., Arbery, M. R., Ashour, N., Barrett, K., Pearse, R., Tio, E., Duong, D., Felsky, D., Jager, P. D., et al. (2024) · Alzheimer's & Dementia · doi:10.1002/alz.13680 · Cited by 19 · see citing works
“28 These cohorts are composed of Catholic nuns, priests, and monks across the United States (ROS) and older persons recruited from communities around the Chicago metropolitan area (MAP).”
BackgroundMethodInfluentialSelf-citationHsieh, Y., Negri, J. M., He, A., Pearse, R. V., Liu, L., Duong, D. M., Chibnik, L. B., Bennett, D. A., Seyfried, N. T., & Young‐Pearse, T. L. (2022) · Molecular Neurodegeneration · doi:10.1186/s13024-022-00558-4 · Cited by 25 · see citing works
“However, over the years of executing this study, extensive data were acquired regarding the human iN system, including data from our lab defining MEA characteristics of iNs over differentiation time [50].”
BackgroundSelf-citationQu, W., Canoll, P., & Hargus, G. (2022) · Neuroscience · doi:10.1016/j.neuroscience.2022.05.006 · Cited by 22 · see citing works
Ng, B., Rowland, H. A., Wei, T., Arunasalam, K., Hayes, E. M., Koychev, I., Hedegaard, A., Ribe, E. M., Chan, D., Chessell, T., et al. (2022) · Brain Communications · doi:10.1093/braincomms/fcac267 · Cited by 18 · see citing works
“It has recently been shown that several measures of secreted Aβ peptides in iPSC-derived cortical neurons from Alzheimer’s disease patients reflect the extent of Aβ neuropathology of their donors.(8) We extend that work on postmortem, end-of-life, neuropathological findings to in-life, early in disease, clinical measu…”
Loss of endosomal exchanger NHE6 leads to pathological changes in tau in human neurons
Fernandez, M. A., Bah, F., Ma, L., Lee, Y., Schmidt, M., Welch, E., Morrow, E. M., & Young‐Pearse, T. L. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.08.001 · Cited by 19 · see citing works
“17 j 2111–2126 j September 13, 2022 (D’Souza et al., 2021; Imberdis et al., 2019; Lagomarsino et al., 2021; Lin et al., 2018; Muratore et al., 2017; Sanyal et al., 2020).”
BackgroundResultInfluentialSelf-citationPatient iPSC models reveal glia-intrinsic phenotypes in multiple sclerosis
Clayton, B. L. L., Barbar, L., Sapar, M. L., Rusielewicz, T., Kalpana, K., Migliori, B., Paull, D., Brenner, K., Moroziewicz, D., Sand, I. K., et al. (2023) · bioRxiv · doi:10.1101/2023.08.01.551553 · Cited by 17
Self-citationSolana‐Manrique, C., Sánchez‐Pérez, A. M., Paricio, N., & Descalzo, S. M. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26020620 · Cited by 19 · see citing works
Zurek, N. A., Ehsanian, R., Goins, A., Adams, I. M., Petersen, T., Goyal, S., Shilling, M., Westlund, K., & Alles, S. (2023) · Journal of Pain · doi:10.1016/j.jpain.2023.12.008 · Cited by 16
Zurek, N. A., Ehsanian, R., Goins, A., Adams, I. M., Petersen, T., Goyal, S., Shilling, M., Westlund, K., & Alles, S. (2023) · bioRxiv · doi:10.1101/2023.11.03.565343 · Cited by 15
Predictive network analysis identifies JMJD6 and other potential key drivers in Alzheimer’s disease
Merchant, J. P., Zhu, K., Henrion, M., Zaidi, S. S. A., Lau, B., Moein, S., Alamprese, M. L., Pearse, R. V., Bennett, D. A., Ertekin‐Taner, N., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-04791-5 · Cited by 22 · see citing works
Self-citationChen, C., Maner-Smith, K. M., Khadka, M., Ahn, J. Y., Gulbin, X. L., Ivanova, A., Dammer, E. B., Seyfried, N. T., Bennett, D. A., Hajjar, I. M., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-64328-8 · Cited by 18 · see citing works
Self-citationMishra, S., Jayadev, S., & Young, J. E. (2024) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2022.0389 · Cited by 13 · see citing works
“…the CNS and the periphery can be differentiated from hiPSCs, the cells are easily genetically manipulatable using CRISPR/Cas9 technology, characteristics of individual patient genomes are maintained in differentiated cells [61] and these cells are widely used in drug testing and screening [62,63].”
BackgroundArtificial intelligence unifies knowledge and actions in drug repositioning
Yin, Z., & Wong, S. T. C. (2021) · Emerging Topics in Life Sciences · doi:10.1042/etls20210223 · Cited by 14 · see citing works
Decoding microglial functions in Alzheimer’s disease: insights from human models
Rao, C., Semrau, S., & Fossati, V. (2025) · Trends in Immunology · doi:10.1016/j.it.2025.02.011 · Cited by 13 · see citing works
Brain Microvascular Pericyte Pathology Linking Alzheimer's Disease to Diabetes
El‐Ghazawi, K., Eyo, U. B., & Peirce, S. M. (2024) · Microcirculation · doi:10.1111/micc.12877 · Cited by 14 · see citing works
Gould, N. L., Scherer, G., Carvalho, S., Shurrush, K. A., Kayyal, H., Edry, E., Elkobi, A., David, O., Foqara, M., Thakar, D., et al. (2023) · Journal of Clinical Investigation · doi:10.1172/jci162120 · Cited by 13 · see citing works
“Currently, major efforts are being made to find new ways to slow metabolic aging (51), particularly in the post-mitotic neurons of the brain.”
BackgroundLee, H., Pearse, R. V., Lish, A. M., Pan, C., Augur, Z. M., Terzioğlu, G., Gaur, P., Liao, M., Fujita, M., Tio, E. S., et al. (2025) · Glia · doi:10.1002/glia.24677 · Cited by 22 · see citing works
Self-citationWang, Z., Jin, M., Hong, W., Liu, W., Reczek, D., Lagomarsino, V. N., Hu, Y., Weeden, T., Frosch, M. P., Young‐Pearse, T. L., et al. (2023) · Acta Neuropathologica Communications · doi:10.1186/s40478-023-01511-2 · Cited by 13 · see citing works
“The neurite number and expression of neural markers reached maximal levels by iN day 14 and by iN day 21 spontaneous firing of the iNs had plateaued [19, 23].”
BackgroundSelf-citationCohen, B. M., & Sonntag, K. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.105238 · Cited by 12 · see citing works
“15,16,18,36,39,41,43,45,47,50,59,61 Elevated levels were more often observed with greater duration of culture, suggesting that increases in abnormal peptides are a consequence of more basic preceding mechanisms.”
BackgroundResultAssociation of AK4 Protein From Stem Cell–Derived Neurons With Cognitive Reserve
Yu, L., Hsieh, Y., Pearse, R., Wang, Y.-L., Petyuk, V., Schneider, J., Buchman, A., Seyfried, N., Jager, P. D. D., Young-Pearse, T. L., et al. (2022) · Neurology · doi:10.1212/wnl.0000000000201120 · Cited by 11 · see citing works
“Generation of iPSC Lines and iNs Technical details on the generation of iPSC lines and iNs are previously described.(10) In brief, the iPSC lines were generated using cryopreserved PBMCs from autopsied ROSMAP participants.”
BackgroundMethodInfluentialSelf-citationUrine-derived cells from the aged donor for the 2D/3D modeling of neural cells via iPSCs
Supakul, S., Hatakeyama, Y., Leventoux, N., Itsuno, M., Numata, N., Hiramine, H., Morimoto, S., Iwata, A., Maeda, S., & Okano, H. (2023) · Aging Brain · doi:10.1016/j.nbas.2023.100101 · Cited by 11 · see citing works
“In fact, some previous studies demonstrated that the neuronal models derived from the iPSCs could recapitulate the disease pathogenesis in relation to the polygenic risks [34,35].”
BackgroundMamais, A., Sanyal, A., Fajfer, A., Zykoski, C. G., Guldin, M., Riley-DiPaolo, A., Subrahmanian, N., Gibbs, W. S., Lin, S., & LaVoie, M. (2023) · bioRxiv · doi:10.1101/2023.04.30.538317 · Cited by 10
Approaches for studying neuroimmune interactions in Alzheimer’s disease
Lin, C., Tian, Y., Tanzi, R. E., & Jorfi, M. (2024) · Trends in Immunology · doi:10.1016/j.it.2024.10.002 · Cited by 10 · see citing works
Oakley, D. H., Chung, M., Abrha, S., Hyman, B. T., & Frosch, M. P. (2024) · Journal of Neuropathology & Experimental Neurology · doi:10.1093/jnen/nlae053 · Cited by 9 · see citing works
Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain
Singh, V., Menard, M. A., Serrano, G., Beach, T., Zhao, H. T., Riley-DiPaolo, A., Subrahmanian, N., LaVoie, M. J., & Volpicelli-Daley, L. A. (2023) · Acta Neuropathologica Communications · doi:10.1186/s40478-023-01704-9 · Cited by 9 · see citing works
“These cells were induced to differentiate into neurons as reported [33, 47, 69] and immunofluorescence experiments were performed using the pRab10 antibody.”
MethodPomeshchik, Y., Velásquez, E., Gil, J., Klementieva, O. L., Gidlöf, R., Sydoff, M., Bagnoli, S., Nacmias, B., Sorbi, S., Westergren‐Thorsson, G., et al. (2023) · Acta Neuropathologica Communications · doi:10.1186/s40478-023-01649-z · Cited by 9 · see citing works
“Recently, Lago-marsino and colleagues generated induced cortical neurons from iPSC lines obtained from a large cohort of deeply phenotyped individuals, including sixteen neu-ropathologically diagnosed with AD and Alzheimer’s dementia [77].”
BackgroundCell models for Down syndrome-Alzheimer’s disease research
Wu, Y., West, N. R., Bhattacharyya, A., & Wiseman, F. K. (2022) · Neuronal Signaling · doi:10.1042/ns20210054 · Cited by 17 · see citing works
INPP5D/SHIP1 regulates inflammasome activation in human microglia
Chou, V., Fancher, S. B., Pearse, R. V., Lee, H., Lam, M., Seyfried, N. T., Bennett, D. A., De Jager, P. L., Menon, V., & Young-Pearse, T. L. (2023) · doi:10.1101/2023.02.25.530025 · Cited by 8 · see citing works
Self-citationMamais, A., Sanyal, A., Fajfer, A., Zykoski, C. G., Guldin, M., Riley-DiPaolo, A., Subrahmanian, N., Gibbs, W. S., Lin, S., & LaVoie, M. J. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.01.001 · Cited by 20 · see citing works
Revisiting a Telencephalic Extent of the Ascending Reticular Activating System
Miyawaki, E. K., Bhattacharyya, S., & Torre, M. (2023) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-023-01339-3 · Cited by 9 · see citing works
Fine-mapping in admixed populations using CARMA-X, with applications to Latin American studies
Yang, Z., Wang, C., Posadas‐García, Y. S., Añorve-Garibay, V., Vardarajan, B. N., Moreno‐Estrada, A., Sohail, M., Mayeux, R. P., & Ionita‐Laza, I. (2025) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2025.02.020 · Cited by 5 · see citing works
Self-citationAge-Related Neurodegenerative Diseases: A Stem Cell’s Perspective
Calvo, B., Schembri-Wismayer, P., & Alonso, M. B. D. (2025) · Cells · doi:10.3390/cells14050347 · Cited by 9 · see citing works
Santarriaga, S., Gerlovin, K., Layadi, Y., & Karmacharya, R. (2023) · Schizophrenia Research · doi:10.1016/j.schres.2023.02.029 · Cited by 7 · see citing works
Brain Endothelial Soluble ST2 Production and Cerebral Edema in a Rat Model of Ischemic Stroke
Bevers, M. B., An, J., Yoo, A., Fietsam, E., Booraem, C., Reddy, D., Li, K., Sastre, C., Acharjee, A., Sugimoto, K., et al. (2025) · Annals of Neurology · doi:10.1002/ana.78006 · Cited by 4 · see citing works
Self-citationYang, T., Xu, Y. R., Jin, S., Ramalingam, N., Bellier, J., Lish, A. M., Ostaszewski, B. L., Young‐Pearse, T. L., Liu, L., Yang, H., et al. (2025) · Alzheimer s Research & Therapy · doi:10.1186/s13195-025-01802-x · Cited by 7 · see citing works
“iPSC-derived neurons (iNs) were differentiated as reported [28] with minor modifications [29]. iPSCs were plated at a density of 95 k cells/cm 2 on plates coated with growth factor reduced Matrigel one day prior to virus transduction (Corning #354,230).”
Self-citationMaguire, E., Winston, J., Ellwood, S. H., O’Donoghue, R., Shaw, B., Castillo-Morales, A., Keat, S., Evans, A., Marshall, I. J., Luckcuck, L., et al. (2025) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102570 · Cited by 7 · see citing works
Changes in iPSC-astrocyte morphology reflect Alzheimer’s disease patient clinical markers
Rowland, H. A., Miller, G., Liu, Q., Li, S., Sharp, N. R., Ng, B., Wei, L., Arunasalam, K., Koychev, I. G., Hedegaard, A., et al. (2024) · Stem Cells · doi:10.1093/stmcls/sxae085 · Cited by 8 · see citing works
“3,4 These findings open the feasibility of modeling sAD, allowing the interrogation of mechanisms and identification of therapeutic targets in a personalized medicine approach.”
White, A. J., Clark, K. A., Alexander, K. D., Ramalingam, N., Young‐Pearse, T. L., Dettmer, U., Selkoe, D. J., & Ho, G. P. H. (2024) · npj Parkinson s Disease · doi:10.1038/s41531-024-00725-y · Cited by 6 · see citing works
“They allow for investigations in a human cell background, exhibit characteristics of excitatory neurons of the layer II/III neocortex 16 , and become electrically active within 21 days of differentiation 17 .”
Self-citationNguyen, L. D., Wei, Z., Silva, M. C., Barberán-Soler, S., Zhang, J., Rabinovsky, R., Muratore, C., Stricker, J. M. S., Hortman, C., Young‐Pearse, T. L., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-43293-0 · Cited by 50 · see citing works
“Induced pluripotent stem cell (iPSC) lines were retrieved and differentiated into neurons with NGN2 expression, as previously reported 27 .”
InfluentialSelf-citationAugur, Z. M., Fogo, G. M., Arbery, M. R., Hsieh, Y., Rao, N. R., Goyal, K., Dexter, E. X., Bennett, D. A., Savas, J. N., Stern, A. M., et al. (2025) · Acta Neuropathologica · doi:10.1007/s00401-025-02947-7 · Cited by 5 · see citing works
“Previously reported iPSC lines were used, which were generated from cryopreserved peripheral blood mononuclear cell (PBMC) samples from autopsied participants from the ROS and MAP cohorts using the Sendai reprogramming method [46].”
InfluentialSelf-citationBenoit, C. R., Sattler, L. B., Aylward, A. J., Pembridge, O. G., Kim, B., Muratore, C., Liao, M., He, A., Ashour, N., Fancher, S. B., et al. (2025) · Brain · doi:10.1093/brain/awaf221 · Cited by 3 · see citing works
“47,54 Astrocytes (ebAstros) were differentiated from NPCs as described.”
Self-citationLee, H., Pearse, R., Lish, A. M., Pan, C., Augur, Z. M., Terzioglu, G., Gaur, P., Liao, M., Fujita, M., Tio, E., et al. (2024) · bioRxiv · doi:10.1101/2024.07.31.605928 · Cited by 5
“We next differentiated iPSCs from 44 individuals, sourced from the Religious Order Studies and Rush Memory and Aging Project (ROSMAP (Lagomarsino et al., 2021)).”
MethodInfluentialSelf-citationZhai, T., Krass, E., Zhang, F., & Huang, Z. J. (2023) · Frontiers in Systems Biology · doi:10.3389/fsysb.2023.1188430 · Cited by 5 · see citing works
“Researchers can use induced pluripotent stem cells (iPSC) to grow human neurons in a dish now (Lagomarsino, 2021).”
BackgroundLish, A. M., Galle, P. C., Orme, G. A., Ashour, N., Heuer, S. E., Curle, A. J., Muratore, C., & Young‐Pearse, T. L. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.104152 · Cited by 6 · see citing works
Self-citationMamais, A., Batchelor, R. D., Chairmandurai, A., Ladd, T. B., Shute, A. J., Subrahmanian, N., Maio, N., Vulpe, C. D., & LaVoie, M. J. (2025) · bioRxiv · doi:10.1101/2025.09.26.678370 · Cited by 4
“BR33 were derived from a Caucasian donor, who was deeply phenotyped as part of the ROS/MAP longitudinal aging studies (53,54) and determined to not be cognitively impaired at death at age >89 and free from genetic variants that confer risk of PD. ND35371 (R1441C heterozygous) and ND50051 (G2019S…”
NCRAD: Advancing Alzheimer's research through high‐quality biospecimens and data
Edler, M. C., Faber, K., Lacy, K., Jackson, J. M., & Foroud, T. (2025) · Alzheimer's & Dementia · doi:10.1002/alz.70682 · Cited by 4 · see citing works
Chun, M. Y., Jung, S., Choe, J., Lee, S., Kim, H., Son, H. J., Choi, Y., Cho, M., Kim, B., Jang, H., et al. (2025) · Alzheimer s & Dementia · doi:10.1002/alz.70660 · Cited by 2 · see citing works
“16 Although numerous studies have investigated the role of single genetic factor such as APOE in AD using organoid models, 18–20 disease Study (BICWALZS), 22 which constituted Dataset 2 for replication analysis.”
Su, W.-S., Wu, M.-T., Cheng, I. H.-J., & Yang, F. (2025) · Experimental Neurology · doi:10.1016/j.expneurol.2025.115420 · Cited by 5 · see citing works
Schwarze‐Taufiq, T. A., Pranoto, I. K. A., Hui, K., Kinoshita, C., Yu, O., Crane, P. K., Gray, S. L., & Young, J. E. (2025) · Alzheimer s & Dementia Translational Research & Clinical Interventions · doi:10.1002/trc2.70040 · Cited by 2 · see citing works
“33”
Schultz, S. A., Liu, L., Schultz, A. P., Fitzpatrick, C. D., Levin, R., Bellier, J., Shirzadi, Z., Joseph‐Mathurin, N., Chen, C. D., Benzinger, T. L. S., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.07.04.547688 · Cited by 5 · see citing works
Self-citationINPP5D/SHIP1 regulates endo-lysosomal function and cargo-selective phagocytosis in human microglia
Terzioglu, G., Karp, E. S., Heuer, S. E., Chadarevian, J. P., Ahsan, F. M., Haage, V. C., Davtyan, H., Fogo, G. M., Curle, A. J., Ashour, N., et al. (2025) · doi:10.1101/2025.10.27.684632 · Cited by 3 · see citing works
Self-citationSaguer, I. L., Gabriele, R. M. C., Bradshaw, T. Y., Leckey, C. A., Belder, C. R. S., Chávez‐Gutiérrez, L., de Silva, R., Fox, N. C., Wray, S., Oxtoby, N. P., et al. (2026) · Brain Communications · doi:10.1093/braincomms/fcag105 · Cited by 3 · see citing works
“A first step on this path would be to test the five A β peptides in iPSC models of LOAD, such as those developed in the literature, 27,28 and patient-derived blood plasma.”
Sanyal, A., Scanavachi, G., Somerville, E., Aylan, B., Costa-Filho, J. I., Brooks, F. A., Dickson, J. R., Hyman, B. T., & Kirchhausen, T. L. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.11.02.686178 · Cited by 2 · see citing works
Liu, J., Zhao, W., Zhang, Z., Ai, X., Cao, B., Zhang, Z., & Ma, D. (2025) · Stem Cell Reviews and Reports · doi:10.1007/s12015-025-10931-7 · Cited by 1 · see citing works
“Ensuring that these induced neurons exhibit senescence and epigenetic characteristics akin to mature substantia nigra neurons in patients remains a critical area for investigation [117].”
Ortlund, E. A., Chen, C.-Y., Maner-Smith, K., Khadka, M., Ahn, J., Gulbin, X., Ivanova, A., Dammer, E., Seyfried, N., Bennett, D. A., et al. (2024) · Research Square · doi:10.21203/rs.3.rs-3973736/v1 · Cited by 3
Self-citationSit, T. P. H., Feord, R., Dunn, A. W. E., Chabros, J., Oluigbo, D., Smith, H., Burn, L., Chang, E., Boschi, A., Yuan, Y., et al. (2024) · bioRxiv · doi:10.1101/2024.02.05.578738 · Cited by 3 · see citing works
“2D human iPSC-derived neuronal cultures show a developmental increase in firing rates and bursting activity that may reveal disease-related phenotypes at the microscale 16,17 .”
Telpoukhovskaia, M. A., Murdy, T. J., Marola, O. J., Charland, K., MacLean, M., Luquez, T., Lish, A. M., Neuner, S. M., Dunn, A. R., Onos, K. D., et al. (2024) · Alzheimer s & Dementia Translational Research & Clinical Interventions · doi:10.1002/trc2.12458 · Cited by 2 · see citing works
“…stem cell (iPSC)-derived neurons from 53 religious orders study and memory aging project (ROSMAP) participants for whom deep cognitive phenotyping and brain-tissue data areavailableweredevelopedandcharacterized 45 includingindividuals cognitively resilient and susceptible to AD pathology.”
BackgroundMethodInfluentialSelf-citation
- Authors
- Lagomarsino, V. N., Pearse, R. V., Liu, L., Hsieh, Y.-C., Fernandez, M. A., Vinton, E. A., Paull, D., Felsky, D., Tasaki, S., Gaiteri, C., Vardarajan, B., Lee, H., Muratore, C. R., Benoit, C. R., Chou, V., Fancher, S. B., He, A., Merchant, J. P., Duong, D. M., Martinez, H., Zhou, M., Bah, F., Vicent, M. A., Stricker, J. M. S., Xu, J., Dammer, E. B., Levey, A. I., Chibnik, L. B., Menon, V., Seyfried, N. T., De Jager, P. L., Noggle, S., Selkoe, D. J., Bennett, D. A., & Young-Pearse, T. L.
- Affiliations
- Brigham and Women's Hospital (US)
- New York Stem Cell Foundation (US)
- Centre for Addiction and Mental Health (CA)
- Rush University Medical Center (US)
- Columbia University Irving Medical Center (US)
- Emory University (US)
- Harvard University (US)
- ORCID
- Lagomarsino, Valentina N.
- Pearse, Richard V.
- Liu, Lei
- Hsieh, Yi-Chen
- Fernandez, Marty A.
- Vinton, Elizabeth A.
- Paull, Daniel
- Felsky, Daniel
- Tasaki, Shinya
- Gaiteri, Chris
- Vardarajan, Badri
- Lee, Hyo
- Benoit, Courtney R.
- Chou, Vicky
- Merchant, Julie P.
- Duong, Duc M.
- Stricker, Jonathan M.S.
- Xu, Jishu
- Dammer, Eric B.
- Levey, Allan I.
- Chibnik, Lori B.
- Menon, Vilas
- Seyfried, Nicholas T.
- Noggle, Scott
- Selkoe, Dennis J.
- Bennett, David A.
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2021-11
- Volume
- 109
- Issue
- 21
- Pages
- 3402-3420.e9
- ISSN
- 0896-6273 (ISSN-L)
- PMC
- PMC8571042
- OpenAlex
- W3198364341
- Cited by
- 151
- References
- 73
- Citations by year
- 2026: 15 · 2025: 37 · 2024: 36 · 2023: 37 · 2022: 20 · 2021: 6
- FWCI
- 16.97
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 367 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R21MH118576, R01AG055909, RF1AG057440, R01AG061798, U01AG046170, R21AG056011, U01AG061356, R01NS117446, R01AG061894)
- National Institute on Aging (R01AG30146, R01AG36836, U01AG32984, U01AG46152, R01AG15819, R01AG017917, P30AG10161)
- New York Stem Cell Foundation
- Lawrence Ellison Foundation
- Illinois Department of Public Health
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- Alzheimer's disease, iPSC-derived neurons, genetic risk, amyloid-beta, tau protein, amyloid plaques, neurofibrillary tangles, cognitive decline, neuropathology, proteomic profiling, RNA profiling, protein networks, brain tissue
- MeSH terms
- Aged, Alzheimer Disease, Cognition, Humans, Neurons, Amyloid beta-Peptides, tau Proteins, Proteomics, Induced Pluripotent Stem Cells
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