Genetically encoded sensors for measuring histamine release both in vitro and in vivo
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Abstract
Histamine (HA) is a key biogenic monoamine involved in a wide range of physiological and pathological processes in both the central and peripheral nervous systems. Because the ability to directly measure extracellular HA in real time will provide important insights into the functional role of HA in complex circuits under a variety of conditions, we developed a series of genetically encoded G-protein-coupled receptor-activation-based (GRAB) HA (GRAB HA) sensors with good photostability, sub-second kinetics, nanomolar affinity, and high specificity. Using these GRAB HA sensors, we measured electrical-stimulation-evoked HA release in acute brain slices with high spatiotemporal resolution. Moreover, we recorded HA release in the preoptic area of the hypothalamus and prefrontal cortex during the sleep-wake cycle in freely moving mice, finding distinct patterns of HA dynamics between these specific brain regions. Thus, GRAB HA sensors are robust tools for measuring extracellular HA transmission in both physiological and pathological processes.
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Directly verifying the transient suppression of spontaneous histamine release in vivo remains technically challenging.
“directly verifying the transient suppression of spontaneous histamine release in vivo remains technically challenging.56”
From Infraslow histaminergic dynamics govern priming states to gate moment-to-moment memory accessibility (2026) · cites this paper as [56]
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Infraslow histaminergic dynamics govern priming states to gate moment-to-moment memory accessibility
Morishita, Y., Takamura, Y., Nishimura, K., Yokoi, Y., Ishihama, Y., Idutsu, R., Ono, M., Matsumoto, R., Hitora-Imamura, N., Minami, M., et al. (2026) · Neuron · doi:10.1016/j.neuron.2026.05.019
- Authors
- Dong, H., Li, M., Yan, Y., Qian, T., Lin, Y., Ma, X., Vischer, H. F., Liu, C., Li, G., Wang, H., Leurs, R., & Li, Y.
- Affiliations
- Peking University (CN)
- Vrije Universiteit Amsterdam (NL)
- Corresponding author
- Li, Yulong
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2023-05
- Volume
- 111
- Issue
- 10
- Pages
- 1564-1576.e6
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W4324349269
- Cited by
- 67
- References
- 50
- Citations by year
- 2026: 15 · 2025: 22 · 2024: 21 · 2023: 9
- FWCI
- 8.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 382 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Neuron (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- VU Research Portal (publishedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- National Natural Science Foundation of China (31925017)
- Fudan University
- Beijing Municipal Science and Technology Commission (Z220009)
- National Key Research and Development Program of China
- National Key Research and Development Program of China Stem Cell and Translational Research (2019YFE011781)
- National Institutes of Health (1U01NS113358, 1U01NS120824)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- histamine, genetically encoded biosensors, G protein-coupled receptor activation, histamine release, acute brain slices, preoptic area, hypothalamus, prefrontal cortex, sleep-wake cycle, freely moving mice, photostability
- MeSH terms
- Animals, Brain, Histamine, Histamine Release, Hypothalamus, Receptors, G-Protein-Coupled, Mice
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