PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome
Open accessAbstract
G protein-coupled receptors (GPCRs) are essential mediators of cellular signaling and are important targets of drug action. Of the approximately 350 nonolfactory human GPCRs, more than 100 are still considered to be 'orphans' because their endogenous ligands remain unknown. Here, we describe a unique open-source resource that allows interrogation of the druggable human GPCRome via a G protein-independent β-arrestin-recruitment assay. We validate this unique platform at more than 120 nonorphan human GPCR targets, demonstrate its utility for discovering new ligands for orphan human GPCRs and describe a method (parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation (PRESTO-Tango)) for the simultaneous and parallel interrogation of the entire human nonolfactory GPCRome.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
52 non-olfactory GPCRs are categorized as Tdark, and new screening platforms have begun the process of illuminating them.
“Although 52 non-olfactory GPCRs are categorized as Tdark, the availability of new screening platforms to discover chemical matter for these GPCRs has begun the process of illumination64,67,68.”
From Unexplored therapeutic opportunities in the human genome (2018) · cites this paper as [67]
The Results and Discussion describe PRESTO-TANGO screening of poorly annotated and orphan GPCRs and the discovery of new ligand–receptor interactions, supporting the claim that a screening platform has begun to illuminate understudied receptors. The paper does not give a count of 52 non-olfactory GPCRs or classify them as Tdark; its Abstract instead discusses more than 100 orphan GPCRs. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Massagué, J. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.753 · Cited by 7,571 · see citing works
Hopkins, A. L., & Groom, C. R. (2002) · Nature Reviews Drug Discovery · doi:10.1038/nrd892 · Cited by 3,305 · see citing works
Transduction of Receptor Signals by ß-Arrestins
Lefkowitz, R. J., & Shenoy, S. K. (2005) · Science · doi:10.1126/science.1109237 · Cited by 1,729 · see citing works
Predicting new molecular targets for known drugs
Keiser, M. J., Setola, V., Irwin, J. J., Laggner, C., Abbas, A. I., Hufeisen, S. J., Jensen, N. H., Kuijer, M. B., Matos, R. C., Tran, T. B., et al. (2009) · Nature · doi:10.1038/nature08506 · Cited by 1,667 · see citing works
Self-citationStructure of the human κ-opioid receptor in complex with JDTic
Wu, H., Wacker, D., Mileni, M., Katritch, V., Han, G. W., Vardy, E., Liu, W., Thompson, A. A., Huang, X., Carroll, F. I., et al. (2012) · Nature · doi:10.1038/nature10939 · Cited by 885 · see citing works
Self-citationJordan, M., Schallhorn, A., & Wurm, F. M. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.4.596 · Cited by 862 · see citing works
Sensory Neuron-Specific GPCR Mrgprs Are Itch Receptors Mediating Chloroquine-Induced Pruritus
Liu, Q., Tang, Z., Surdenikova, L., Kim, S., Patel, K., Kim, A., Ru, F., Guan, Y., Weng, H.-J., Geng, Y., et al. (2009) · Cell · doi:10.1016/j.cell.2009.11.034 · Cited by 820 · see citing works
The G protein-coupled receptor repertoires of human and mouse
Vassilatis, D. K., Hohmann, J. G., Zeng, H., Li, F., Ranchalis, J., Mortrud, M. T., Brown, A., Rodriguez, S. S., Weller, J. R., Wright, A. C., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0230374100 · Cited by 779 · see citing works
Structural Features for Functional Selectivity at Serotonin Receptors
Wacker, D., Wang, C., Katritch, V., Han, G. W., Huang, X., Vardy, E., McCorvy, J. D., Jiang, Y., Chu, M., Siu, F., et al. (2013) · Science · doi:10.1126/science.1232808 · Cited by 705 · see citing works
Self-citationThe genetic design of signaling cascades to record receptor activation
Barnea, G., Strapps, W. R., Herrada, G., Berman, Y. L., Ong, J., Kloss, B., Axel, R., & Lee, K. J. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0710487105 · Cited by 673 · see citing works
Angers, S., Salahpour, A., Joly, E., Hilairet, S., Chelsky, D., Dennis, M., & Bouvier, M. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.3684 · Cited by 639 · see citing works
The Repertoire of G-Protein–Coupled Receptors in Fully Sequenced Genomes
Fredriksson, R., & Schiöth, H. B. (2005) · Molecular Pharmacology · doi:10.1124/mol.104.009001 · Cited by 613 · see citing works
Molecular control of δ-opioid receptor signalling
Fenalti, G., Giguère, P. M., Katritch, V., Huang, X., Thompson, A. A., Cherezov, V., Roth, B. L., & Stevens, R. C. (2014) · Nature · doi:10.1038/nature12944 · Cited by 501 · see citing works
Self-citationDiversity and Modularity of G Protein-Coupled Receptor Structures
Katritch, V., Cherezov, V., & Stevens, R. (2011) · TIPS - Trends in Pharmacological Sciences · doi:10.1016/j.tips.2011.09.003 · Cited by 495 · see citing works
Bjarnadóttir, T. K., Gloriam, D. E. I., Hellstrand, S. H., Kristiansson, H., Fredriksson, R., & Schiöth, H. B. (2006) · Genomics · doi:10.1016/j.ygeno.2006.04.001 · Cited by 433 · see citing works
A β-Arrestin/Green Fluorescent Protein Biosensor for Detecting G Protein-coupled Receptor Activation
Barak, L. S., Ferguson, S. S. G., Zhang, J., & Caron, M. G. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.44.27497 · Cited by 428 · see citing works
Edwards, A. M., Isserlin, R., Bader, G. D., Frye, S. V., Willson, T. M., & Yu, F. H. (2011) · Nature · doi:10.1038/470163a · Cited by 411 · see citing works
Allen, J., Yost, J. M., Setola, V., Chen, X., Sassano, M. F., Chen, M., Peterson, S. M., Yadav, P. N., Huang, X., Feng, B., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1104807108 · Cited by 352 · see citing works
Self-citationAngers, S. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.060590697 · Cited by 341 · see citing works
Rask-Andersen, M., Masuram, S., & Schiöth, H. B. (2014) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-011613-135943 · Cited by 282 · see citing works
Guan, X., Kobilka, T. S., & Kobilka, B. K. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41623-7 · Cited by 265 · see citing works
The human genome and drug discovery after a decade. Roads (still) not takenPreprint
Isserlin, R., Bader, G. D., Edwards, A. M., Frye, S. V., Willson, T. M., & Yu, F. H. (2011) · arXiv (Cornell University) · doi:10.48550/arxiv.1102.0448 · Cited by 233 · see citing works
Chimeric G Proteins Allow a High-Throughput Signaling Assay of Gi-Coupled Receptors
Coward, P., Chan, S. D. H., Wada, H., Humphries, G. M. K., & Conklin, B. R. (1999) · Analytical Biochemistry · doi:10.1006/abio.1999.4061 · Cited by 216 · see citing works
Strategies to Discover Unexpected Targets for Drugs Active at G Protein–Coupled Receptors
Allen, J. A., & Roth, B. L. (2011) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-010510-100553 · Cited by 216 · see citing works
Self-citationMechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression
Auld, D. S., Thorne, N., Maguire, W. F., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813345106 · Cited by 208 · see citing works
Cao, W., Luttrell, L. M., Medvedev, A. V., Pierce, K. L., Daniel, K. W., Dixon, T. M., Lefkowitz, R. J., & Collins, S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.c000592200 · Cited by 208 · see citing works
Characterization of Chemical Libraries for Luciferase Inhibitory Activity
Auld, D. S., Southall, N. T., Jadhav, A., Johnson, R. L., Diller, D., Simeonov, A. M., Austin, C. P., & Inglese, J. (2008) · Journal of Medicinal Chemistry · doi:10.1021/jm701302v · Cited by 183 · see citing works
Southern, C., Cook, J. M., Neetoo‐Isseljee, Z., Taylor, D. L., Kettleborough, C. A., Merritt, A. T., Bassoni, D. L., Raab, W. J., Quinn, E. R., Wehrman, T. S., et al. (2013) · SLAS DISCOVERY · doi:10.1177/1087057113475480 · Cited by 181 · see citing works
Differential β-Arrestin Trafficking and Endosomal Sorting of Somatostatin Receptor Subtypes
Tulipano, G., Stumm, R. K., Pfeiffer, M., Kreienkamp, H., Höllt, V., & Schulz, S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313522200 · Cited by 171 · see citing works
Hennen, S., Wang, H., Peters, L., Merten, N., Simon, K., Spinrath, A., Blättermann, S., Akkari, R., Schrage, R., Schröder, R. J., et al. (2013) · Science Signaling · doi:10.1126/scisignal.2004350 · Cited by 143 · see citing works
Identification of Novel Functionally Selective κ-Opioid Receptor Scaffolds
White, K. L., Scopton, A. P., Rives, M., Bikbulatov, R. V., Polepally, P. R., Brown, P. J., Kenakin, T., Javitch, J. A., Zjawiony, J. K., & Roth, B. L. (2013) · Molecular Pharmacology · doi:10.1124/mol.113.089649 · Cited by 142 · see citing works
Self-citationPharmacology and Signaling of MAS-Related G Protein–Coupled Receptors
Solinski, H. J., Gudermann, T. W., & Breit, A. (2014) · Pharmacological Reviews · doi:10.1124/pr.113.008425 · Cited by 131 · see citing works
Vita, N., Oury-Donat, F., Chalon, P., Guillemot, M., Kaghad, M., Bachy, A., Thurneyssen, O., García, S., Poinot‐Chazel, C., Casellas, P., et al. (1998) · European Journal of Pharmacology · doi:10.1016/s0014-2999(98)00678-5 · Cited by 128 · see citing works
A Specific Mechanism for Nonspecific Activation in Reporter-Gene Assays
Auld, D. S., Thorne, N., Nguyễn, Ð.-T., et al. (2008) · ACS Chemical Biology · doi:10.1021/cb8000793 · Cited by 116 · see citing works
Non-invasive optical biosensor for assaying endogenous G protein-coupled receptors in adherent cells
Ye, F., Li, G., & Ferrie, A. M. (2006) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/j.vascn.2006.11.001 · Cited by 92 · see citing works
Eglen, R. M., Bossé, R., & Reisine, T. (2007) · Assay and Drug Development Technologies · doi:10.1089/adt.2007.062 · Cited by 90 · see citing works
Jensen, D. D., Godfrey, C. B., Niklas, C., Canals, M., Kočan, M., Poole, D. P., Murphy, J. E., Alemi, F., Cottrell, G. S., Korbmacher, C., et al. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.455774 · Cited by 89 · see citing works
Richard, F., Barroso, S., Martínez, J., et al. (2001) · Molecular Pharmacology · doi:10.1124/mol.60.6.1392 · Cited by 83 · see citing works
Hill, S. J., Williams, C., & May, L. T. (2010) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2010.00779.x · Cited by 65 · see citing works
Differential β‐arrestin binding of AT1and AT2angiotensin receptors
Turu, G., Szidonya, L. K., Gáborik, Z., Buday, L., Spät, A., Clark, A. J. L., & Hunyady, L. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.11.044 · Cited by 61 · see citing works
Yan, Y.-X., Boldt‐Houle, D. M., Tillotson, B. P., Gee, M. A., D'Eon, B. J., Chang, X.-J., Olesen, C. E. M., & Palmer, M. A. J. (2002) · SLAS DISCOVERY · doi:10.1177/108705702237677 · Cited by 59 · see citing works
Cho, D.-I., Beom, S., Tol, H. H. M. V., Caron, M. G., & Kim, K. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.09.090 · Cited by 52 · see citing works
Stanasila, L., Abuin, L., Dey, J. H., et al. (2008) · Molecular Pharmacology · doi:10.1124/mol.107.043422 · Cited by 49 · see citing works
Kim, K., & Caron, M. G. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.11.055 · Cited by 43 · see citing works
Hanson, B. J., Wetter, J. A., Bercher, M. R., et al. (2009) · SLAS DISCOVERY · doi:10.1177/1087057109335260 · Cited by 34 · see citing works
Emkey, R., & Rankl, N. B. (2009) · Methods in molecular biology · doi:10.1007/978-1-60327-258-2_7 · Cited by 31 · see citing works
Biasing the Prostaglandin F2α Receptor Responses toward EGFR-Dependent Transactivation of MAPK
Goupil, E., Wisehart, V., Khoury, E., Zimmerman, B., Jaffal, S. M., Hébert, T. E., & Laporte, S. A. (2012) · Molecular Endocrinology · doi:10.1210/me.2011-1245 · Cited by 24 · see citing works
Studies of a ubiquitous receptor family
Roth, B. L., & Marshall, F. H. (2012) · Nature · doi:10.1038/492057a · Cited by 18 · see citing works
Self-citationβ-Arrestin-Based Bret2 Screening Assay for the “Non”-β-Arrestin Binding CB1 Receptor
Vrecl, M., Nørregaard, P. K., Almholt, D. L. C., Elster, L., Pogačnik, A., & Heding, A. (2009) · SLAS DISCOVERY · doi:10.1177/1087057109333101 · Cited by 17 · see citing works
Twaites, B. R., Wilton, L. V., Layton, D., et al. (2007) · Acta Diabetologica · doi:10.1007/s00592-007-0010-y · Cited by 14 · see citing works
Analysis of the Coupling of G12/13 to G Protein-Coupled Receptors Using a Luciferase Reporter Assay
Liu, B., & Wu, D. (2003) · Humana Press eBooks · doi:10.1385/1-59259-430-1:145 · Cited by 11 · see citing works
Siehler, S., & Guerini, D. (2006) · Journal of Receptors and Signal Transduction · doi:10.1080/10799890600932246 · Cited by 9 · see citing works
Using Reporter Gene Technologies to Detect Changes in cAMP as a Result of GPCR Activation
Rodrigues, D. J., & McLoughlin, D. J. (2009) · Methods in molecular biology · doi:10.1007/978-1-60327-317-6_23 · Cited by 2 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Roth, B. L. (2016) · Neuron · doi:10.1016/j.neuron.2016.01.040 · Cited by 1,742 · see citing works
Self-citationYoon, H. S., Cho, C. H., Yun, M. S., Jang, S. J., You, H. J., Kim, J.-H., Han, D., Cha, K. H., Moon, S. H., Lee, K., et al. (2021) · Nature Microbiology · doi:10.1038/s41564-021-00880-5 · Cited by 602 · see citing works
A genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine
Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu, Z., Wu, H., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.02.037 · Cited by 545 · see citing works
Structure of a Hallucinogen-Activated Gq-Coupled 5-HT2A Serotonin Receptor
Kim, K., Che, T., Panova, O., DiBerto, J. F., Lyu, J., Krumm, B. E., Wacker, D., Robertson, M. J., Seven, A. B., Nichols, D. E., et al. (2020) · Cell · doi:10.1016/j.cell.2020.08.024 · Cited by 481 · see citing works
Self-citationSynthon-based ligand discovery in virtual libraries of over 11 billion compounds
Sadybekov, A. A., Sadybekov, A. V., Liu, Y., Iliopoulos-Tsoutsouvas, C., Huang, X.-P., Pickett, J., Houser, B., Patel, N., Tran, N. K., Tong, F., et al. (2022) · Nature · doi:10.1038/s41586-021-04220-9 · Cited by 417 · see citing works
“The Tango arrestin recruitment assays were performed as previously described 23 .”
MethodInfluentialSelf-citationUnexplored therapeutic opportunities in the human genome
Oprea, T. I., Bologa, C. G., Brunak, S., Campbell, A., Gan, G. N., Gaulton, A., Gomez, S. M., Guha, R., Hersey, A., Holmes, J., et al. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2018.14 · Cited by 378 · see citing works
Self-citationStructure-based discovery of nonhallucinogenic psychedelic analogs
Cao, D., Yu, J., Wang, H., Luo, Z., Liu, X., He, L., Qi, J., Fan, L., Tang, L., Chen, Z., et al. (2022) · Science · doi:10.1126/science.abl8615 · Cited by 312 · see citing works
In silico design of novel probes for the atypical opioid receptor MRGPRX2
Lansu, K., Karpiak, J., Liu, J., Huang, X.-P., McCorvy, J. D., Kroeze, W. K., Che, T., Nagase, H., Carroll, F. I., Jin, J., et al. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2334 · Cited by 293 · see citing works
Self-citationStructures of the σ2 receptor enable docking for bioactive ligand discovery
Alon, A., Lyu, J., Braz, J. M., Tummino, T. A., Craik, V., O’Meara, M. J., Webb, C. M., Radchenko, D. S., Moroz, Y. S., Huang, X.-P., et al. (2021) · Nature · doi:10.1038/s41586-021-04175-x · Cited by 211 · see citing works
Self-citationA serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility
Sheu, S.-H., Upadhyayula, S., Dupuy, V., Pang, S., Deng, F., Wan, J., Walpita, D., Pasolli, H. A., Houser, J., Sanchez-Martinez, S., et al. (2022) · Cell · doi:10.1016/j.cell.2022.07.026 · Cited by 162 · see citing works
Glimmers in illuminating the druggable genome
Rodgers, G., Austin, C., Anderson, J., Pawlyk, A., Colvis, C., Margolis, R., & Baker, J. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.252 · Cited by 126 · see citing works
Can, A., Zanos, P., Moaddel, R., Kang, H. J., Dossou, K. S. S., Wainer, I. W., Cheer, J. F., Frost, D. O., Huang, X.-P., & Gould, T. D. (2016) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.116.235838 · Cited by 115 · see citing works
Self-citationReprogramming immunosuppressive myeloid cells facilitates immunotherapy for colorectal cancer
Lu, W., Yu, W., He, J., Liu, W., Yang, J., Lin, X., Zhang, Y., Wang, X., Jiang, W., Luo, J., et al. (2021) · EMBO Molecular Medicine · doi:10.15252/emmm.202012798 · Cited by 112 · see citing works
Discovery of new GPCR ligands to illuminate new biology
Roth, B. L., Irwin, J. J., & Shoichet, B. K. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2490 · Cited by 94 · see citing works
Self-citationBaumann, M. H., Majumdar, S., Le Rouzic, V., Hunkele, A., Uprety, R., Huang, X. P., Xu, J., Roth, B. L., Pan, Y.-X., & Pasternak, G. W. (2018) · Neuropharmacology · doi:10.1016/j.neuropharm.2017.08.016 · Cited by 92 · see citing works
Roth, B. L., & Kroeze, W. K. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.r115.654764 · Cited by 88 · see citing works
Self-citationGenetically encoded sensors for measuring histamine release both in vitro and in vivo
Dong, H., Li, M., Yan, Y., Qian, T., Lin, Y., Ma, X., Vischer, H. F., Liu, C., Li, G., Wang, H., et al. (2023) · Neuron · doi:10.1016/j.neuron.2023.02.024 · Cited by 67 · see citing works
Mu-Opioid Receptor Selective Superagonists Produce Prolonged Respiratory Depression
Malcolm, N. J., Palkovic, B., Sprague, D. J., Calkins, M. M., Lanham, J. K., Halberstadt, A. L., Stucke, A. G., & McCorvy, J. D. (2023) · iScience · doi:10.1016/j.isci.2023.107121 · Cited by 53 · see citing works
Self-citationKetamine Metabolite (2<i>R</i>,6<i>R</i>)-Hydroxynorketamine Interacts with μ and κ Opioid Receptors
Joseph, T. T., Bu, W., Lin, W., Zoubak, L., Yeliseev, A., Liu, R., Eckenhoff, R. G., & Brannigan, G. (2021) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.0c00741 · Cited by 28 · see citing works
Liver-expressed antimicrobial peptide 2 elevation contributes to age-associated cognitive decline
Tian, J., Guo, L., Wang, T., Jia, K., Swerdlow, R. H., Zigman, J. M., & Du, H. (2023) · JCI Insight · doi:10.1172/jci.insight.166175 · Cited by 16 · see citing works
Applications and potential of in silico approaches for psychedelic chemistry
Karabulut, S., Kaur, H., & Gauld, J. W. (2023) · Molecules · doi:10.3390/molecules28165966 · Cited by 8 · see citing works
Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides
Hsiao, M.-H., Miao, Y., Liu, Z., Schütze, K., Limjunyawong, N., Chien, D. C.-C., Monteiro, W. D., Chu, L.-S., Morgenlander, W., Jayaraman, S., et al. (2025) · Molecular & Cellular Proteomics · doi:10.1016/j.mcpro.2024.100901 · Cited by 5 · see citing works
- Authors
- Kroeze, W. K., Sassano, M. F., Huang, X.-P., Lansu, K., McCorvy, J. D., Giguère, P. M., Sciaky, N., & Roth, B. L.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Roth, Bryan L
- Venue
- Nature Structural & Molecular Biology
- Published
- 2015-04-20
- Volume
- 22
- Issue
- 5
- Pages
- 362-369
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- PMC
- PMC4424118
- OpenAlex
- W2036251618
- Cited by
- 755
- References
- 53
- Citations by year
- 2026: 77 · 2025: 99 · 2024: 92 · 2023: 95 · 2022: 78 · 2021: 77 · 2020: 58 · 2019: 60 · 2018: 51 · 2017: 44 · 2016: 20 · 2015: 3
- FWCI
- 17.21
- Type
- journal article
- Language
- en
- Update notices
- correction (2024-03-14)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
- UNC Libraries
- Funders
- National Institutes of Health (NIH T32)
- National Institute of Mental Health (U19MH082441, U01MH104974, UO1MH104974)
- National Institute on Drug Abuse (R01DA027170, R01DA27170)
- National Institute of General Medical Sciences (NIH 5-T32-GM007040, T32GM007040)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Physical Sciences › Computer Science › Computational Theory and Mathematics › Computational Drug Discovery Methods
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- G protein-coupled receptors, orphan G protein-coupled receptor, high-throughput screening, β-arrestin recruitment, G protein-independent signaling, ligand discovery, transcriptional activation
- MeSH terms
- beta-Arrestins, Amino Acid Sequence, Biological Assay, Humans, Ligands, Transcription, Genetic, Transcriptional Activation, Arrestins, RNA Interference, RNA, Small Interfering, Receptors, G-Protein-Coupled
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
