The DOI matches its Crossref record; 97 of the 98 references it cites were found in a registry or an index.
Characterization of Kiss1 neurons using transgenic mouse models
Open accessAbstract
Humans and mice with loss-of-function mutations of the genes encoding kisspeptins (Kiss1) or kisspeptin receptor (Kiss1r) are infertile due to hypogonadotropic hypogonadism. Within the hypothalamus, Kiss1 mRNA is expressed in the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (Arc). In order to better study the different populations of kisspeptin cells we generated Kiss1-Cre transgenic mice. We obtained one line with Cre activity specifically within Kiss1 neurons (line J2-4), as assessed by generating mice with Cre-dependent expression of green fluorescent protein or β-galactosidase. Also, we demonstrated Kiss1 expression in the cerebral cortex and confirmed previous data showing Kiss1 mRNA in the medial nucleus of amygdala and anterodorsal preoptic nucleus. Kiss1 neurons were more concentrated towards the caudal levels of the Arc and higher leptin-responsivity was observed in the most caudal population of Arc Kiss1 neurons. No evidence for direct action of leptin in AVPV Kiss1 neurons was observed. Melanocortin fibers innervated subsets of Kiss1 neurons of the preoptic area and Arc, and both populations expressed melanocortin receptors type 4 (MC4R). Specifically in the preoptic area, 18-28% of Kiss1 neurons expressed MC4R. In the Arc, 90% of Kiss1 neurons were glutamatergic, 50% of which also were GABAergic. In the AVPV, 20% of Kiss1 neurons were glutamatergic whereas 75% were GABAergic. The differences observed between the Kiss1 neurons in the preoptic area and the Arc likely represent neuronal evidence for their differential roles in metabolism and reproduction.
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“Even though estradiol is considered the main regulator of gonadotropin release, GnRH and kisspeptin neurons express various glutamate and GABA receptors and respond to these neurotransmitters [100,101].”
From Fibromyalgia pathogenesis explained by a neuroendocrine multistable model (2024) · cites this paper as [101]
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Citations
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Metadata
- Authors
- Cravo, R. M., Margatho, L. O., Osborne-Lawrence, S., Donato, J., Atkin, S., Bookout, A. L., Rovinsky, S., Frazão, R., Lee, C. E., Gautron, L., Zigman, J. M., & Elias, C. F.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Universidade de São Paulo (BR)
- Corresponding author
- Elias, C.F.
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 173
- Pages
- 37-56
- ISSN
- 0306-4522 (ISSN-L)
- PMC
- PMC3026459
- OpenAlex
- W1992174470
- Cited by
- 353
- References
- 98
- Citations by year
- 2026: 7 · 2025: 16 · 2024: 21 · 2023: 21 · 2022: 24 · 2021: 35 · 2020: 18 · 2019: 34 · 2018: 18 · 2017: 22 · 2016: 19 · 2015: 22 · 2014: 29 · 2013: 29 · 2012: 18
- FWCI
- 25.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Drug Abuse (R01DA024680)
- National Institute of General Medical Sciences (T32GM007062, GM007062)
- National Institute of Diabetes and Digestive and Kidney Diseases (K08DK068069)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD061539)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Keywords
- kisspeptin neurons, KISS1, KISS1R, arcuate nucleus, anteroventral periventricular nucleus, preoptic area, medial amygdala, melanocortin-4 receptor, glutamatergic neurons, GABAergic neurons, hypothalamus, hypogonadotropic hypogonadism, reproduction, metabolism
- MeSH terms
- Animals, Brain, Cell Separation, Disease Models, Animal, Female, Flow Cytometry, Immunohistochemistry, Male, Mice, Inbred C57BL, Mice, Transgenic, Neurons, Proteins, In Situ Hybridization, Reverse Transcriptase Polymerase Chain Reaction, Mice, Kisspeptins
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