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Organotypic brain slice cultures: A review

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Humpel, C.·2015·Neuroscience
DOI 10.1016/j.neuroscience.2015.07.086PMC PMC4699268
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Abstract

In vitro cell cultures are an important tool for obtaining insights into cellular processes in an isolated system and a supplement to in vivo animal experiments. While primary dissociated cultures permit a single homogeneous cell population to be studied, there is a clear need to explore the function of brain cells in a three-dimensional system where the main architecture of the cells is preserved. Thus, organotypic brain slice cultures have proven to be very useful in investigating cellular and molecular processes of the brain in vitro. This review summarizes (1) the historical development of organotypic brain slices focusing on the membrane technology, (2) methodological aspects regarding culturing procedures, age of donors or media, (3) whether the cholinergic neurons serve as a model of neurodegeneration in Alzheimer's disease, (4) or the nigrostriatal dopaminergic neurons as a model of Parkinson's disease and (5) how the vascular network can be studied, especially with regard to a synthetic blood-brain barrier. This review will also highlight some limits of the model and give an outlook on future applications.

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Cited by

Sorted by citations

Authors
Humpel, C.
Affiliations
Innsbruck Medical University (AT)
Corresponding author
Humpel, C.
ORCID
Humpel, C.
Venue
Neuroscience
Publisher
Elsevier BV
Published
2015-10
Volume
305
Pages
86-98
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/j.neuroscience.2015.07.086
PMC
PMC4699268
OpenAlex
W1112818150
Cited by
480
References
4
Citations by year
2026: 38 · 2025: 54 · 2024: 46 · 2023: 56 · 2022: 50 · 2021: 50 · 2020: 52 · 2019: 37 · 2018: 40 · 2017: 41 · 2016: 14 · 2015: 2
FWCI
3.84
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuroscience (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund (P24734-B24)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
organotypic brain slice culture, membrane technology, donor age, culture media, cholinergic neurons, Alzheimer's disease, neurodegeneration model, nigrostriatal dopaminergic neurons, Parkinson's disease, blood-brain barrier, cerebral vasculature
MeSH terms
Animals, Brain, Organ Culture Techniques, Dopaminergic Neurons, Cholinergic Neurons, In Vitro Techniques

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