Heat-activated sustaining nitric oxide release from zwitterionic diazeniumdiolate loaded in thermo-sensitive liposomes
Abstract
Zwitterionic diazeniumdiolate, a nitric oxide precursor, was dissolved in basic buffer solution (pH=9.0) and encapsulated in thermo-sensitive liposomes composed of phospholipids of different sensitive temperatures. The basic intra-liposomal environment dramatically delayed spontaneous NO release. When the liposomes were placed in physiological buffer solution and temperatures were increased to the sensitive temperatures of the phospholipids' membranes, extra-liposomal protons started to influx to collapse the pH gradient and subsequently induce a significant NO release. Moreover, the presence of a stronger pH gradient when the liposomes were applied to a more acidic environment caused a higher proton influx driving force to trigger the influx of protons and, subsequently, NO release. In this work, we demonstrate that thermo-sensitive liposomes can be used to create a stable pH gradient in a nanoenvironment entrapping zwitterionic diazeniumdiolate for sustained and heat/acid-activated NO release.
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Encapsulating N-diazeniumdiolates within pH- or thermo-sensitive liposomes achieves controlled release; pH or temperature fluctuations change proton influx through the biolayer membrane and induce significant NO release.
“Other researchers have conducted further studies to encapsulate NO donors within pH126 or thermo-sensitive247 liposomes to achieve controlled release. In short, when N-diazeniumdiolates are encapsulated within liposomes, pH or temperature fluctuations change the proton influx through the biolayer membrane and subsequently induce a significant NO release.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [247]
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- Authors
- Tai, L.-A., Wang, Y.-C., & Yang, C.-S.
- Affiliations
- National Health Research Institutes (TW)
- Corresponding author
- Yang, Chung-Shi
- Venue
- Nitric Oxide
- Publisher
- Elsevier BV
- Published
- 2010-08
- Volume
- 23
- Issue
- 1
- Pages
- 60-64
- ISSN
- 1089-8603 (ISSN-L)
- OpenAlex
- W1975381476
- Cited by
- 54
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 5 · 2016: 6 · 2015: 4 · 2013: 3 · 2012: 4
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nitric Oxide (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Health Research Institutes
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Keywords
- nitric oxide release, pH gradient, proton influx, sustained drug release, liposome encapsulation
- MeSH terms
- Azo Compounds, Hot Temperature, Hydrogen-Ion Concentration, Liposomes, Nitric Oxide, Drug Delivery Systems, Nitric Oxide Donors
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