The Mediterranean and CHO diets decrease VCAM-1 and E-selectin expression induced by modified low-density lipoprotein in HUVECs
Abstract
Background and aim The oxidative modifications of low-density lipoprotein (LDL) are crucial for the atherosclerosis process. The aim of this study was to determine if the minimally modified LDL, obtained after the ingestion of three different diets, produce differential effects on the vascular cell adhesion molecule-1 (VCAM-1) and E-selectin expression in human umbilical endothelial cells (HUVECs).Methods and results Twenty healthy young males were exposed to three dietary periods. Each period lasted four weeks. During the first period, all subjects consumed a saturated fat (SFA) enriched diet (38% fat, 20% SFA). The second and third dietary periods were administered following a randomized crossover design: a low fat high carbohydrates diet (CHO diet) and a Mediterranean diet. LDL particles, isolated during each dietary period, were oxidized by exposure to UV light and incubated for 48 h with HUVEC. Thereafter, 100 U/mL of TNF-alpha was added and incubation continued for 6 h. Cellular ELISA determined adhesion molecules expression. Lag time, propagation rate and total amounts of formed conjugated dienes were calculated in LDL incubated with 10mumol/L Cu(2+). When compared to the SFA diet, LDL isolated from the Mediterranean and CHO diets induced a lower expression of VCAM-1 and E-selectin in HUVECS (P<0.007). There were no differences between both lipid lowering diets. However, lag time of LDL from the Mediterranean diet was higher than with the CHO diet (P<0.042). This parameter was inversely correlated with E-selectin expression (r=-0.497; P<0.04).Conclusion Our results suggest that both the Mediterranean and CHO diets may decrease the pro-inflammatory environment induced by modified LDL in endothelial cells.
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In obese non-diabetic or diabetic individuals, some studies observed improved insulin sensitivity after prolonged neutralizing TNF-a antibody treatment.
“In obese non-diabetic or diabetic individuals, several studies have observed improvement of insulin sensitivity after prolonged treatment with neutralising TNF-a antibodies (150,151) , whereas other trials did not report such effects of TNF-a antibody injections, despite dampening of systemic inflammation (152) .”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [150]
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- Authors
- Bellido, C., López-Miranda, J., Pérez-Martínez, P., Paz, E., Marín, C., Gómez, P., Moreno, J. A., Moreno, R., & Pérez-Jiménez, F.
- Affiliations
- Hospital Universitario Reina Sofía (ES)
- ORCID
- Moreno, Juan A.
- Venue
- Nutrition Metabolism and Cardiovascular Diseases
- Publisher
- Elsevier BV
- Published
- 2006-12
- Volume
- 16
- Issue
- 8
- Pages
- 524-530
- ISSN
- 0939-4753 (ISSN-L)
- OpenAlex
- W2155158678
- Cited by
- 25
- References
- 38
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2020: 1 · 2018: 1 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutrition Metabolism and Cardiovascular Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Keywords
- Mediterranean diet, high-carbohydrate diet, LDL oxidation, modified LDL, VCAM-1, E-selectin, endothelial adhesion molecules, human umbilical vein endothelial cells, dietary saturated fat, atherosclerosis, oxidative stress, TNF-α
- MeSH terms
- Adult, Cells, Cultured, Dietary Carbohydrates, Enzyme-Linked Immunosorbent Assay, Fatty Acids, Humans, Cholesterol, HDL, Lipoproteins, LDL, Cholesterol, LDL, Male, Tumor Necrosis Factor-alpha, Cross-Over Studies, Vascular Cell Adhesion Molecule-1, E-Selectin, Diet, Mediterranean, Endothelial Cells
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