Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans
Open accessAbstract
Objective The goal of this study was to evaluate the effects of creatine (Cr) supplementation on oxidative stress and inflammation markers after acute repeated-sprint exercise in humans.Methods Twenty-five players under age 20 y were randomly assigned to two groups: Cr supplemented and placebo. Double-blind controlled supplementation was performed using Cr (0.3 g/kg) or placebo tablets for 7 d. Before and after 7 d of supplementation, the athletes performed two consecutive Running-based Anaerobic Sprint Tests (RAST). RAST consisted of six 35-m sprint runs at maximum speed with 10 sec rest between them. Blood samples were collected just prior to start of test (pre), just after the completion (0 h), and 1 h after completion.Results Average, maximum, and minimum power values were greater in the Cr-supplemented group compared with placebo (P < 0.05). There were significant increases (P < 0.05) in plasma tumor necrosis factor alpha (TNF-α) and C-reactive protein (CRP) up to 1 h after acute sprint exercise in the placebo-supplemented group. Malondialdehyde, lactate dehydrogenase (LDH), catalase, and superoxide dismutase enzymes also were increased after exercise in both groups. Red blood cell glutathione was lower after exercise in both groups. Cr supplementation reversed the increase in TNF-α and CRP as well as LDH induced by acute exercise. Controversially, Cr supplementation did not inhibit the rise in oxidative stress markers. Also, antioxidant enzyme activity was not different between placebo and Cr-supplemented groups.Conclusion Cr supplementation inhibited the increase of inflammation markers TNF-α and CRP, but not oxidative stress markers, due to acute exercise.
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It may also explain reports that creatine supplementation attenuates inflammatory and/or muscle damage in response to intense exercise.
“It may also explain reports that creatine supplementation attenuates inflammatory and/or muscle damage in response to intense exercise [274,295,296,297].”
From Creatine in health and disease (2021) · cites this paper as [296]
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Cites
Aebi, H. (1984) · Methods in Enzymology · doi:10.1016/s0076-6879(84)05016-3 · Cited by 25,696 · see citing works
Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.
Paglia, D. E., & Valentine, W. N. (1967) · PubMed · Cited by 10,776 · see citing works
Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration.
Dill, D. B., & COSTILL, D. L. (1974) · Journal of Applied Physiology · doi:10.1152/jappl.1974.37.2.247 · Cited by 3,391 · see citing works
Rahman, I., Kode, A., & Biswas, S. K. (2006) · Nature Protocols · doi:10.1038/nprot.2006.378 · Cited by 2,262 · see citing works
Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation
Harris, R. C., Söderlund, K., & Hultman, E. (1992) · Clinical Science · doi:10.1042/cs0830367 · Cited by 1,141 · cited 2× · see citing works
“Although the current supplementation regime has been shown to “load” muscle cells to threshold levels [1,15], a major limitation of the present study was the lack of Cr concentration measurement in skeletal muscle.”
BackgroundNielsen, F., Mikkelsen, B. B., Nielsen, J. B., Andersen, H. R., & Grandjean, P. (1997) · Clinical Chemistry · doi:10.1093/clinchem/43.7.1209 · Cited by 1,017 · see citing works
Muscle creatine loading in men
Hultman, E., Soderlund, K., Timmons, J. A., Cederblad, G., & Greenhaff, P. L. (1996) · Journal of Applied Physiology · doi:10.1152/jappl.1996.81.1.232 · Cited by 822 · see citing works
Direct antioxidant properties of creatine
Lawler, J. M., Barnes, W. S., Wu, G., Song, W., & Demaree, S. (2002) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.6164 · Cited by 448 · see citing works
Zagatto, A. M., Beck, W. R., & Gobatto, C. A. (2009) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e3181b3df32 · Cited by 349 · see citing works
Creatine supplementation with specific view to exercise/sports performance: An update
Cooper, R., Naclerio, F., Allgrove, J., & Jimenez, A. (2012) · Journal of the International Society of Sports Nutrition · doi:10.1186/1550-2783-9-33 · Cited by 306 · see citing works
Sestili, P., Martinelli, C., Bravi, G., Piccoli, G., Curci, R., Battistelli, M., Falcieri, E., Agostini, D., Gioacchini, A. M., & Stocchi, V. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.10.035 · Cited by 220 · see citing works
Wyss, M., & Schulze, A. (2002) · Neuroscience · doi:10.1016/s0306-4522(02)00088-x · Cited by 210 · see citing works
Sestili, P., Martinelli, C., Colombo, E., Barbieri, E., Potenza, L., Sartini, S., & Fimognari, C. (2011) · Amino Acids · doi:10.1007/s00726-011-0875-5 · Cited by 200 · see citing works
Increased release of ATP from endothelial cells during acute inflammation
Bodin, P., & Burnstock, G. (1998) · Inflammation Research · doi:10.1007/s000110050341 · Cited by 193 · see citing works
Santos, R. V. T., Bassit, R. A., Caperuto, E. C., & Costa Rosa, L. F. B. P. (2004) · Life Sciences · doi:10.1016/j.lfs.2003.11.036 · Cited by 162 · see citing works
Chapter 1 Effect Of Exercise On Oxidative Stress Biomarkers
Bloomer, R. J. (2008) · Advances in clinical chemistry · doi:10.1016/s0065-2423(08)00401-0 · Cited by 145 · see citing works
Bassit, R. A., Curi, R., & Costa Rosa, L. F. B. P. (2008) · Amino Acids · doi:10.1007/s00726-007-0582-4 · Cited by 120 · see citing works
Rahimi, R. (2011) · Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e3182162f2b · Cited by 100 · see citing works
Bassit, R. A., Pinheiro, C. H. D. J., Vitzel, K. F., Sproesser, A. J., Silveira, L. R., & Curi, R. (2009) · European Journal of Applied Physiology · doi:10.1007/s00421-009-1305-1 · Cited by 91 · see citing works
Lenz, H., Schmidt, M., Welge, V., Schlattner, U., Wallimann, T., Elsässer, H.-P., Wittern, K.-P., Wenck, H., Stäb, F., & Blatt, T. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2004.23522.x · Cited by 89 · see citing works
Differential effect of creatine on oxidatively-injured mitochondrial and nuclear DNA
Guidi, C., Potenza, L., Sestili, P., Martinelli, C., Guescini, M., Stocchi, L., Zeppa, S. D., Polidori, E., Annibalini, G., & Stocchi, V. (2007) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2007.09.018 · Cited by 84 · see citing works
Anti‐inflammatory activity of creatine supplementation in endothelial cells in vitro
Nomura, A., Zhang, M., Sakamoto, T., Ishii, Y., Morishima, Y., Mochizuki, M., Kimura, T., Uchida, Y., & Sekizawa, K. (2003) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0705316 · Cited by 73 · see citing works
Effects of creatine supplementation on homocysteine levels and lipid peroxidation in rats
Deminice, R., Portari, G. V., Vannucchi, H., & Jordão, A. A. (2008) · British Journal Of Nutrition · doi:10.1017/s0007114508162985 · Cited by 71 · see citing works
Self-citationCreatine supplementation reduces oxidative stress biomarkers after acute exercise in rats
Deminice, R., & Jordão, A. A. (2011) · Amino Acids · doi:10.1007/s00726-011-1121-x · Cited by 58 · see citing works
Self-citationPlasma guanidino compounds are altered by oral creatine supplementation in healthy humans
Derave, W., Marescau, B., Eynde, E. V., Eijnde, B. O., Deyn, P. P. D., & Hespel, P. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00206.2004 · Cited by 57 · see citing works
Guimarães‐Ferreira, L., Pinheiro, C. H. D. J., Gerlinger‐Romero, F., Vitzel, K. F., Nachbar, R. T., Curi, R., & Nunes, M. T. (2012) · European Journal of Applied Physiology · doi:10.1007/s00421-012-2378-9 · Cited by 55 · see citing works
Protective effect of creatine against RNA damage
Fimognari, C., Sestili, P., Lenzi, M., Cantelli‐Forti, G., & Hrelia, P. (2009) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2009.07.005 · Cited by 47 · see citing works
Role of Creatine Supplementation on Exercise‐Induced Cardiovascular Function and Oxidative Stress
Kingsley, M., Cunningham, D. J., Mason, L., Kilduff, L. P., & McEneny, J. (2009) · Oxidative Medicine and Cellular Longevity · doi:10.4161/oxim.2.4.9415 · Cited by 34 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- [20]20. Costa CM, Dos Santos RCC, Lima E. A simple automated procedure for thiol measurement in human and serum samples. Braz J Pathol Lab Med 2006;42:345-50.
- [21]21. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
Cited by
Sorted by citations
IOC Consensus Statement: Dietary Supplements and the High-performance Athlete
Maughan, R. J., Burke, L. M., Dvorak, J., Larson-Meyer, D. E., Peeling, P., Phillips, S. M., Rawson, E. S., Walsh, N. P., Garthe, I., Geyer, H., et al. (2018) · British Journal of Sports Medicine · doi:10.1136/bjsports-2018-099027 · Cited by 1,004 · see citing works
Kreider, R. B., Kalman, D. S., Antonio, J., Ziegenfuss, T. N., Wildman, R., Collins, R., Candow, D. G., Kleiner, S. M., Almada, A. L., & Lopez, H. L. (2017) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-017-0173-z · Cited by 854 · see citing works
IOC Consensus Statement: Dietary Supplements and the High-Performance Athlete
Maughan, R. J., Burke, L. M., Dvorak, J., Larson-Meyer, D. E., Peeling, P., Phillips, S. M., Rawson, E. S., Walsh, N. P., Garthe, I., Geyer, H., et al. (2018) · International Journal of Sport Nutrition and Exercise Metabolism · doi:10.1123/ijsnem.2018-0020 · Cited by 540 · see citing works
Creatine in health and disease
Kreider, R. B., & Stout, J. R. (2021) · Nutrients · doi:10.3390/nu13020447 · Cited by 227 · see citing works
Evidence-based supplements for the enhancement of athletic performance
Peeling, P., Binnie, M. J., Goods, P. S. R., Sim, M., & Burke, L. M. (2018) · International Journal of Sport Nutrition and Exercise Metabolism · doi:10.1123/ijsnem.2017-0343 · Cited by 225 · see citing works
Creatine for exercise and sports performance, with recovery considerations for healthy populations
Wax, B., Kerksick, C. M., Jagim, A. R., Mayo, J. J., Lyons, B. C., & Kreider, R. B. (2021) · Nutrients · doi:10.3390/nu13061915 · Cited by 182 · see citing works
Heaton, L. E., Davis, J. K., Rawson, E. S., Nuccio, R. P., Witard, O. C., Stein, K. W., Baar, K., Carter, J. M., & Baker, L. B. (2017) · Sports Medicine · doi:10.1007/s40279-017-0759-2 · Cited by 158 · see citing works
Candow, D. G., Forbes, S. C., Chilibeck, P. D., Cornish, S. M., Antonio, J., & Kreider, R. B. (2019) · Journal of Clinical Medicine · doi:10.3390/jcm8040488 · Cited by 149 · see citing works
Peeling, P., Castell, L. M., Derave, W., de Hon, O., & Burke, L. M. (2019) · International Journal of Sport Nutrition and Exercise Metabolism · doi:10.1123/ijsnem.2018-0271 · Cited by 116 · see citing works
Wallimann, T., Riek, U., & Möddel, M. (2017) · Medical Hypotheses · doi:10.1016/j.mehy.2016.12.002 · Cited by 87 · see citing works
Energy Systems Contribution in the Running-based Anaerobic Sprint Test
Milioni, F., Zagatto, A., Barbieri, R., Andrade, V., dos Santos, J., Gobatto, C., da Silva, A., Santiago, P., & Papoti, M. (2017) · International Journal of Sports Medicine · doi:10.1055/s-0042-117722 · Cited by 72 · see citing works
Cella, P. S., Marinello, P. C., Borges, F. H., Ribeiro, D. F., Chimin, P., Testa, M. T. J., Guirro, P. B., Duarte, J. A., Cecchini, R., Guarnier, F. A., et al. (2020) · European Journal of Nutrition · doi:10.1007/s00394-019-01933-6 · Cited by 51 · see citing works
Exploratory studies of the potential anti-cancer effects of creatine
Campos-Ferraz, P. L., Gualano, B., das Neves, W., Andrade, I. T., Hangai, I., Pereira, R. T. S., Bezerra, R. N., Deminice, R., Seelaender, M., & Lancha, A. H. (2016) · Amino Acids · doi:10.1007/s00726-016-2180-9 · Cited by 47 · see citing works
Self-citationRole of creatine supplementation in exercise-induced muscle damage: A mini review
Kim, J., Lee, J., Kim, S., Yoon, D., Kim, J., & Sung, D. J. (2015) · Journal of Exercise Rehabilitation · doi:10.12965/jer.150237 · Cited by 35 · see citing works
Cornish, S. M., & Peeler, J. D. (2018) · Nutrition Research · doi:10.1016/j.nutres.2017.12.010 · Cited by 19 · see citing works
- Authors
- Deminice, R., Rosa, F. T., Franco, G. S., Jordao, A. A., & de Freitas, E. C.
- Affiliations
- Universidade Estadual de Londrina (BR)
- Universidade de São Paulo (BR)
- Corresponding author
- Deminice, Rafael
- Venue
- Nutrition
- Publisher
- Elsevier BV
- Published
- 2013-09
- Volume
- 29
- Issue
- 9
- Pages
- 1127-1132
- ISSN
- 0899-9007 (ISSN-L)
- OpenAlex
- W2049848088
- Cited by
- 135
- References
- 28
- Citations by year
- 2026: 14 · 2025: 8 · 2024: 6 · 2023: 8 · 2022: 12 · 2021: 14 · 2020: 12 · 2019: 10 · 2018: 16 · 2017: 11 · 2016: 10 · 2015: 10 · 2014: 4
- FWCI
- 8.55
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Keywords
- creatine supplementation, oxidative stress, inflammation, repeated sprint exercise, TNF-α, C-reactive protein, malondialdehyde, lactate dehydrogenase, antioxidant enzymes, glutathione, youth athletes
- MeSH terms
- Adolescent, Antioxidants, C-Reactive Protein, Creatine, Double-Blind Method, Humans, Inflammation, L-Lactate Dehydrogenase, Male, Malondialdehyde, Running, Soccer, Superoxide Dismutase, Tumor Necrosis Factor-alpha, Biomarkers, Oxidative Stress, Dietary Supplements, Sports Nutritional Physiological Phenomena
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