Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: Validity of clinical data depends on vacutainer system used
Abstract
Ascorbate and dehydroascorbic acid are frequently used as biomarkers of oxidative stress, but their lack of stability ex vivo and rapid postsampling interconversion continue to result in erroneous reference values. One problem is the large variety of vacutainer devices used for blood sampling purposes and the basic question of plasma vs serum as matrix. This study acquired blood samples by using 9 different and commonly used vacutainer systems followed by acidic stabilization and analysis by a well-validated method with the purpose of identifying acceptable means of collecting samples for proper ascorbate/dehydroascorbic acid analysis. In comparison, K(3)-EDTA vacutainers were superior in maintaining low ex vivo oxidation of vitamin C.
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Heparin and ethylenediaminetetraacetic acid (EDTA) on ice are the most suitable anticoagulants since L-ascorbic acid is unstable with gel or fluoride anticoagulants.
“Heparin and ethylenediaminetetraacetic acid (EDTA) on ice are the most suitable since L-ascorbic acid is unstable with gel or fluoride anticoagulants [215].”
From Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination (2021) · cites this paper as [215]
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Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination
Doseděl, M., Jirkovský, E., Macáková, K., Krčmová, L., Javorská, L., Pourová, J., Mercolini, L., Remião, F., Nováková, L., Mladěnka, P., et al. (2021) · Nutrients · doi:10.3390/nu13020615 · Cited by 553 · see citing works
Myths, Artifacts, and Fatal Flaws: Identifying Limitations and Opportunities in Vitamin C Research
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- Authors
- Lykkesfeldt, J.
- Affiliations
- University of Copenhagen (DK)
- Corresponding author
- Lykkesfeldt, Jens
- ORCID
- Lykkesfeldt, Jens
- Venue
- Nutrition Research
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 32
- Issue
- 1
- Pages
- 66-69
- ISSN
- 0271-5317 (ISSN-L)
- OpenAlex
- W2005651031
- Cited by
- 43
- References
- 12
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 6 · 2016: 4 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 3
- FWCI
- 5.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutrition Research (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- vitamin C, dehydroascorbic acid, oxidative stress biomarkers, blood sampling, vitamin C stability, reference values
- MeSH terms
- Ascorbic Acid, Blood Specimen Collection, Dehydroascorbic Acid, Heparin, Humans, Biomarkers, Oxidative Stress
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