Cited by 40Antimicrobial Peptides and ActivitiesCoconut Research and ApplicationsMicrobial Natural Products and Biosynthesis

An antifungal peptide from the coconut

Wang, H.X., Ng, T.B.·2005·Peptides

Abstract

A chromatographic procedure consisting of ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast performance liquid chromatography on Supedex 75 was utilized to isolate a 10 kDa antifungal peptide from coconut flesh. The peptide was unadsorbed on DEAE-cellulose, but adsorbed on Affi-gel blue gel and CM-cellulose. It displayed antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. The IC50 values of its inhibitory activities on mycelial growth in M. arachidicola and HIV-1 reverse transcriptase activity were respectively 1.2 and 52.5 microM.

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Crossref · 10.1016/j.peptides.2005.05.009

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51 of 51 references verified0 citation errors · 0 not checkable

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Authors
Wang, H. X., & Ng, T. B.
Affiliations
  • State Key Laboratory of Agricultural Biotechnology (CN)
  • Chinese University of Hong Kong (HK)
Corresponding author
Ng, T.B.
Venue
Peptides
Publisher
Elsevier BV
Published
2005-12
Volume
26
Issue
12
Pages
2392-2396
ISSN
0196-9781 (ISSN-L)
OpenAlex
W2087990733
Cited by
40
References
51
Citations by year
2025: 2 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 2 · 2020: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 1 · 2013: 1 · 2012: 2
FWCI
0.84
Type
journal article
Language
en
Open access
closed
Locations
  • Peptides (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Physical Sciences › Chemistry › Inorganic Chemistry › Coconut Research and Applications
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
Keywords
antifungal peptides, peptide isolation, Fusarium oxysporum, Physalospora piricola, HIV-1 reverse transcriptase inhibition, mycelial growth inhibition
MeSH terms
Antifungal Agents, Cocos, Dose-Response Relationship, Drug, Fusarium, Microbial Sensitivity Tests, Nuts, Peptides, Plant Proteins, Reverse Transcriptase Inhibitors, HIV Reverse Transcriptase

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