Effect of ornithine decarboxylase and norspermidine in modulating cell division in the green alga Chlamydomonas reinhardtii
Open accessAbstract
The extensive genetic resources of Chlamydomonas has led to its widespread use as a model system for understanding fundamental processes in plant cells, including rates of cell division potentially modulated through polyamines. Putrescine was the major polyamine in both free (88%) and membrane-bound fractions (93%) while norspermidine was the next most abundant in these fractions accounting for 11% and 6%, respectively. Low levels of diaminopropane, spermidine and spermine were also observed although no cadaverine or norspermine were detected. Ornithine decarboxylase (ODC, EC 4.1.1.17) activity was almost five times higher than arginine decarboxylase (ADC, EC 4.1.1.19) and is the major route of putrescine synthesis. The fluoride analogue of ornithine (α-DFMO) inhibited membrane associated ODC activity whilst simultaneously stimulating cell division in a dose dependent manner. Following exposure to α-DFMO the putrescine content in the cells declined while the norspermidine content increased over two fold. Addition of norspermidine to cultures stimulated cell division mimicking the effects observed using DFMO and also reversed the inhibitory effects of cyclohexylamine on growth. The results reveal that ODC is the major route to polyamine formation in the Chlamydomonas CC-406 cell-wall mutant, in contrast to the preferential ADC route reported for Chlorella vulgaris, suggesting that significant species differences exist in biosynthetic pathways which modulate endogenous polyamine levels in green algae.
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Optimal growth of algae may require supplementation with a wide range of micronutrients such as those found in Hutner's trace elements solution.
“optimal growth may require supplementation with a wide range of micronutrients such as those found in Hutner's trace elements solution”
From Key Targets for Improving Algal Biofuel Production (2021) · cites this paper as [25]
The cultivation methods (accepted manuscript, p. 5) say that Chlamydomonas was grown in TAP medium containing Hutner’s trace elements. The paper does not establish that those elements are required for optimal growth, or assess a wide range of micronutrients. (LLM, conf=0.84)
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Key Targets for Improving Algal Biofuel Production
Griffiths, G., Hossain, A. K., Sharma, V., & Duraisamy, G. (2021) · Clean Technologies · doi:10.3390/cleantechnol3040043 · Cited by 49 · see citing works
Self-citationAwad, N., Vega-Estévez, S., & Griffiths, G. (2020) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2020.02.019 · Cited by 20 · see citing works
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- Authors
- Tassoni, A., Awad, N., & Griffiths, G.
- Affiliations
- University of Bologna (IT)
- Aston University (GB)
- Corresponding author
- Tassoni, Annalisa
- Griffiths, Gareth
- Venue
- Plant Physiology and Biochemistry
- Publisher
- Elsevier BV
- Published
- 2018-02
- Volume
- 123
- Pages
- 125-131
- ISSN
- 0981-9428 (ISSN-L)
- OpenAlex
- W2773090100
- Cited by
- 28
- References
- 41
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 1
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Plant Physiology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Aston Publications Explorer (Aston University) (acceptedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion, cc-by-nc-nd)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protist diversity and phylogeny
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- ornithine decarboxylase, norspermidine, polyamines, cell division, Chlamydomonas reinhardtii, putrescine, arginine decarboxylase, cyclohexylamine, polyamine biosynthesis, green algae
- MeSH terms
- Cell Division, Mutation, Ornithine Decarboxylase, Plant Proteins, Putrescine, Chlamydomonas reinhardtii
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