Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
Abstract
Objective Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n=85), and war trauma-exposed individuals with current PTSD (n=113), with life-time PTSD (n=61) and without PTSD (trauma controls; n=88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD.Methods Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting.Results Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (Bmax) in PBMCs. Hormone-binding potential (Bmax/KD ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between Bmax and KD that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting Bmax data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD.Conclusions Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between Bmax and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.
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Elevated glucocorticoid receptor (GR) expression levels in lymphocytes occur in tandem with enhanced glucocorticoid sensitivity in PTSD.
“Heightened levels of peripheral and central corticotropin-releasing hormone (CRH; Baker et al, 2005;de Kloet et al, 2008) and elevated glucocorticoid receptor (GR) expression levels in lymphocytes (Matic et al, 2013) occur in tandem with enhanced glucocorticoid sensitivity in PTSD.”
From Inflammation in Fear- and Anxiety-Based Disorders: PTSD, GAD, and Beyond (2017) · cites this paper as Matic et al, 2013
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- Authors
- Matić, G., Milutinović, D. V., Nestorov, J., Elaković, I., Jovanović, S. M., Perišić, T., Dunđerski, J., Damjanović, S., Knežević, G., Špirić, Ž., Vermetten, E., & Savić, D.
- Affiliations
- University of Belgrade (RS)
- Military Medical Academy (RS)
- University Medical Center Utrecht (NL)
- Corresponding author
- Matić, Gordana
- ORCID
- Venue
- Progress in Neuro-Psychopharmacology and Biological Psychiatry
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 43
- Pages
- 238-245
- ISSN
- 0278-5846 (ISSN-L)
- OpenAlex
- W2154640175
- Cited by
- 43
- References
- 62
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 6 · 2017: 4 · 2016: 8 · 2015: 4 · 2014: 3 · 2013: 4
- FWCI
- 7.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Progress in Neuro-Psychopharmacology and Biological Psychiatry (publishedVersion)
- PubMed (publishedVersion)
- Faculty of Philosophy (University of Belgrade) (publishedVersion)
- Digital Repository of Archived Publications (University of Belgrade, Institute for Biological Research Sinisa Stankovic) (submittedVersion)
- VinaR (Institute of Nuclear Sciences "Vinča") (submittedVersion)
- Funders
- European Commission (INCO-CT-2004-509213)
- Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja (179018, 41009)
- Topics
- Social Sciences › Psychology › Clinical Psychology › Posttraumatic Stress Disorder Research
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- post-traumatic stress disorder, glucocorticoid receptor, war trauma, peripheral blood mononuclear cells, receptor expression, hormone binding affinity, receptor protein levels
- MeSH terms
- Adult, Analysis of Variance, Dexamethasone, Electrophoresis, Polyacrylamide Gel, Glucocorticoids, Hormones, Humans, Kinetics, Lymphocytes, Male, Middle Aged, Muramidase, Radioligand Assay, Receptors, Glucocorticoid, Stress Disorders, Post-Traumatic, Warfare, Wounds and Injuries, Blotting, Western, Diagnostic and Statistical Manual of Mental Disorders, Serbia, Real-Time Polymerase Chain Reaction
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